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Sakura Tissue-Tek TEC 6, Operating Manua

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Sakura Tissue-Tek TEC 6 Operating Manua Download Page 61

3. Explanation of Operations

61

7.  Place the tissue into the base mold and slightly press to the bottom by 

forceps.

  
8.  Cool the bottom of the base mold with the cold spot, and push the tissue 

with a tamper to fix its position. (Cool the bottom of the base mold until a 
thin layer of solidified paraffin is formed.)

9.  Place the cassette on the base mold with the tissue side up.

 
10. Press the finger plate and add paraffin to around 80% of the depth of the 

cassette.

 

コールドスポット

タンパー

Tamper

Cold spot

カセットの深さの、

8 分目程度までパラフィンを入れる

Add paraffin to around 80% of the 
depth of the cassette.

Summary of Contents for Tissue-Tek TEC 6

Page 1: ...Tissue Tek TEC 6 Embedding Module Operating Manual Part Number M01 021E 02 Revision Letter A Released 2019 11 07 ...

Page 2: ...ppear here Sakura Tissue Tek Please visit our website www sakura finetek com Manufactured for Sakura Finetek USA Inc Torrance CA 90501 U S A Sakura Finetek Europe B V 2408 AV Alphen aan den Rijn NL Sakura Finetek Japan Co Ltd Tokyo 103 0023 Japan Manufactured by Sakura Seiki Co Ltd Nagano 387 0015 Japan 2018 Sakura Finetek Japan Co Ltd All Rights Reserved Printed in Japan ...

Page 3: ...ent 33 2 1 2 Installation Environment 33 2 1 3 Unpacking Method of the Embedding Module 34 2 1 4 Installation Method 38 2 2 Setting Up the Instrument 41 2 2 1 Temperature Setting for Each Part of the Instrument 41 2 2 2 Setting the Date and Time 44 2 2 3 Setting the Schedule of the Auto Operation with the Auto Cryo Mode 45 2 2 4 Setting the Display Language 47 2 2 5 Setting the Auto Panel Display ...

Page 4: ...g 4 1 Error Log 75 4 1 1 Viewing the Error Log 75 5 Troubleshooting 5 1 Troubleshooting 77 5 1 1 Instrument Condition and Solution 77 5 1 2 Message Window 80 5 1 3 What to Do Following a Power Outage 85 5 1 4 What to Do When the Low Battery Alarm Icon is Indicated 85 5 2 Cleaning 86 5 2 1 Inspection and Cleaning 86 5 2 2 Cleaning Each Part of the Embedding Module 86 6 Replacement Parts 6 1 Replace...

Page 5: ...Table of contents 5 ...

Page 6: ...1 Introduction ...

Page 7: ...ical studies and tests conducted in the fields of pathology anatomy clinical pathology etc This instrument can be used standalone and also designed for use with the Cryo Module connected by a control cable This document is the Operating Manual for the Embedding Module but the procedures to handle the Tissue Tek TEC 6 Cryo Module are also provided Carefully read this Operating Manual and use the in...

Page 8: ...njury and property damage These instructions provide important safety information that must be needed at all times In this manual instructions pertaining to different levels of potential hazards are classified and indicated respectively as danger warning caution and note The definition of each hazard is defined below DANGER pertains to a potential hazard where failure to observe the specified inst...

Page 9: ...mbol specifies an action that must be taken Always follow the instruction A label bearing this symbol specifies a prohibited item Always follow the instruction A label bearing this symbol specifies a precaution Wrong handling against the instruction may potentially expose the operator to danger or damage the instrument so be sure to follow the instruction A label bearing this symbol is provided ne...

Page 10: ...and paraffin container in the instrument become hot Exercise caution to prevent burns Do not disassemble or modify the instrument Do not disassemble or modify the instrument except for the areas specified in this manual The instrument may malfunction or cause accident thus creating a dangerous situation Do not wet the instrument Do not wet the instrument except for the areas specified in this manu...

Page 11: ...ted dust and or paraffin periodically Failure to do so may cause fire depending on the condition Keep the instrument with the optional magnifying lens away from direct sunlight exposure The lens subjected to direct sunlight may cause fire and or accident because of refraction of light ...

Page 12: ...ct the Sakura instrument distributor or representative Do not remove the warning caution labels Do not remove the warning caution labels attached to the instrument Without these labels the operators cannot be reminded of the necessary warning cautionary information in the day to day handling of the instrument which may result in unexpected problems Perform periodic inspection of the instrument eve...

Page 13: ... put down the instrument in an unstable place or on a floor or surface with insufficient strength Stay away from the instrument during earthquake The instrument may fall over and cause injury Disconnection from power source Turn the power switch off before unplugging the power cord ...

Page 14: ...e paraffin melting chamber paraffin and paraffin container in the instrument become hot Wear rubber gloves or other protective gears to prevent burns Also make sure the instrument is used only by those with appropriate knowledge and education Caution for high temperature When using the instrument do not directly touch the molten paraffin in the paraffin chamber warming chamber etc You may get burn...

Page 15: ...ecautions when cleaning Caution for chemical agent When cleaning do not use chemical agents other than those specified Unauthorized chemical agents may damage the instrument or cause health issues Also wear protective gears and ventilate the work area when cleaning Items to check when cleaning paraffin drain trays Discard the paraffin in the paraffin drain trays every time after using the instrume...

Page 16: ...rned when the paraffin is solid failure may result Do not close the paraffin flow rate adjustment dial after work If the paraffin cools down and solidifies when the flow rate adjustment dial is fully closed the paraffin may expand the next time it is melted and leak out from the flow rate adjustment dial Do not put anything on top of the instrument Failure or unexpected problems may occur Do not p...

Page 17: ... extending it using a different cord etc If the power cord is extended and connected the supply voltage may drop and the compressor may stop working This is especially true with the Cryo Module Do not use things with a sharp point such as a sharp pen tip to operate the control panel display Doing so will cause instrument failure Intake port Cold spot ...

Page 18: ...fin block is removed clean the inside of the base mold If the inside of the base mold is not clean the paraffin block may not be removed easily in the next session Clean the instrument or equipment properly when it gets dirty during the embedding Continuing the embedding work with dirty instrument or equipment may cause contamination Paraffin attached on the hot plate may cause a base mold to move...

Page 19: ...mbedding A cold spot used for positioning and orienting the tissue A dispenser that dispenses paraffin for embedding A cooling plate that cools solidifies the embedded paraffin block and Electronic controls control panel display and other parts for temperature controlling monitoring or otherwise manipulating the aforementioned components Embedding Module CAUTION The instrument is designed for use ...

Page 20: ...e humidity 30 to 85 non condensing Altitude 2 000m or lower Storage environment Ambient temperature 10 C to 60 C Relative humidity 30 to 85 non condensing Overvoltage category II 2 Transient overvoltage up to the level 1500V Electromagnetic environment Normal electromagnetic environment Pollution degree 2 per IEC61010 Noise 60 dB or less A weighted level per JIS Z8737 2 2000 Vibration Drop Vibrati...

Page 21: ... 1 1 1 2 Scraper 1550 1 1 1 1 1 3 Tamper large 1551 1 each 1 each 1 each 1 each 1 each Tamper small 1552 4 Power cord A4010541 1 A4010537 1 A4010534 1 1 1 5 Wrist rest R 5121 1 each 1 each 1 each 1 each 1 each Wrist rest L 5122 6 Wrist rest cushion R 5123 1 each 1 each 1 each 1 each 1 each Wrist rest cushion L 5124 Operating manual 1 1 1 1 1 1 3 4 TEC 6 EM J0 2 L L R R 5 6 TEC 6 EM A1 TEC 6 EM E2 ...

Page 22: ...ame Product code Quantity TEC 6 EM J0 TEC 6 EM A1 TEC 6 EM E2 TEC 6 EM JC2 TEC 6 EM J2 1 Foot pedal 5785 1 1 1 1 1 2 Tray small 5782 1 1 1 1 1 3 Base mold dividers 5783 1 1 1 1 1 4 Magnifying lens assembly with wrench and dust cover 5127 1 1 1 1 1 1 3 4 2 ...

Page 23: ...itch A switch for the main power supply The lamp is turned on while power is supplied 4 Paraffin flow rate adjustment dial A dial for adjusting the amount of paraffin to be dispensed 5 Dispenser A dispenser that dispenses paraffin 6 Finger plate A switch for dispensing paraffin used for embedding Pressing the switch starts dispensing and releasing it stops dispensing Dispensing will stop after the...

Page 24: ...port This port is used to take in air to raise the cooling efficiency of the cold spot 11 Cold spot A cooling plate used to quickly cool paraffin at the bottom of a base mold during embedding to help correctly position the tissue sample 12 Hot plate A work surface on which embedding is performed 13 Hand rest A resin part that prevents accidental contact with the hot plate during embedding 1 6 2 In...

Page 25: ...omes on when the voltage in the battery of the display becomes low When this icon comes on the battery must be replaced Refer to 5 1 4 What to Do When the Low Battery Alarm Icon is Indicated 4 Date day of the week and time area The current date day of the week and time are displayed 5 Cryo button An operation switch for the Cryo Module Touching this button when the icon is gray changes the icon co...

Page 26: ...the highest level is reached the next touch of the button adjusts the brightness to the lowest level Refer to 3 2 4 Turning On Off the Work Light for details Touch Touch Touch Touch Touch Touch Level1 Level2 Level3 Level4 Level5 Level1 9 Main Menu button Touch this button to move to the Main Menu screen On the Main Menu screen you can switch between auto operation and manual operation perform quic...

Page 27: ...yed separately for the left and right but the temperature setting is the same 15 Cold spot A cooling spot The cold spot temperature cannot be set 15 C or below 16 Start time display area Auto operation preparing operating The day of the week and time at which the current operation was started are displayed Auto operation paused The day of the week and time at which the next operation will be start...

Page 28: ...ore the end time arrives in auto operation Refer to 3 2 5 Continuing Embedding after the End Time of Auto Operation for details This area is not displayed during manual operation 20 Lock Display button Hold down this button to lock the operation of the display Holding down it for at least 3 seconds locks the display To unlock the display hold down the button again for at least 3 seconds Hold this ...

Page 29: ... and warming chambers by 5 C Touching the button again during quick heating cancels quick heating Refer to 3 2 2 Melting Paraffin Quickly for details This button is not displayed while the instrument is paused 4 Cleaning mode button Use this button to lower the temperatures of the forceps holder and hot plate This button is not displayed while the instrument is paused Refer to 2 2 5 Setting the Au...

Page 30: ...used to set the order of year month and day and the 24 hour display or 12 hour display mode Refer to 3 2 1 Various Settings for details 4 Auto Panel Display Off Settings button This button can be used to set the time until the panel display turns off The panel display can also be set to never turn off Refer to 2 2 5 Setting the Auto Panel Display Off Timer for details 5 Error Log button Use this b...

Page 31: ...1 Introduction 31 ...

Page 32: ...2 Installation ...

Page 33: ...r or dropped its internal components may malfunction or get damaged Always exercise due care when handling the instrument 2 1 2 Installation Environment Install the instrument in a location meeting the following conditions Free from fire or ignition sources nearby Not exposed to direct sunlight A horizontal level and stable surface area Subject to minimum vibration Protected from water splashes Aw...

Page 34: ...orces applied during transport Unpacking of the instrument should be performed by staff trained in the instrument The instrument itself weighs approx 25 kg At least two people should work together when lifting or moving the instrument When lifting the instrument bend your knees fully or otherwise assume a natural posture Do not put down the instrument in an unstable place or on a floor with insuff...

Page 35: ...lifting the instrument because the center of gravity is located on the rear side of the instrument Do not hold the instrument with your hands on the front side hand rest Exercise caution that lifting the instrument with your fingers hooked underneath may result in pinched fingers when the instrument is set down Side pad L Side pad R Hand rest CAUTION ...

Page 36: ... tapes attached on the lid of the warming chamber 2 Take the left and right warming chamber lids out of the plastic bags 3 The tray large is found inside the right warming chamber Remove it from Warming Chamber and take it out of the plastic bag TEC 6 EM J0 There is another warming chamber tray large in the left warming chamber Paraffin drain tray Take the paraffin drain tray out of the plastic ba...

Page 37: ...ment distributor or representative Name of part Quantity Remarks TEC 6 EM J0 TEC 6 EM A1 TEC 6 EM E2 TEC 6 EM JC2 TEC 6 EM J2 Power cord 1 1 1 1 1 Housed in the instrument Warming chamber lid 2 2 2 2 2 Tray large 2 1 1 1 1 Scraper 1 1 1 1 1 Tamper large 1 1 1 1 1 Tamper small 1 1 1 1 1 Paraffin drain tray 2 2 2 2 2 Wrist rest R 1 each 1 each 1 each 1 each 1 each Wrist rest L Wrist rest cushion R 1...

Page 38: ...he power plug and confirm no molten paraffin inside the instrument or wait for it to cool and solidify Installing the Instrument 1 Select a work bench that has enough space to allow the Embedding Module and Cryo Module to be placed on and enough strength to withstand the mass of the instrument containing paraffin approx 55 kg The left right and rear side of the instrument can be in contact with wa...

Page 39: ...ar of the instrument so do not place the instrument close to the walls yet Connecting the Cables 1 Confirm that the respective power switch of the Embedding Module and Cryo Module has been turned OFF 2 Connect the control cable while paying attention to the insertion direction 3 Insert the power cord of each module into an applicable power inlet Work Flow Work Flow ...

Page 40: ... connect remove the power plug with a wet hand Failure to observe the instruction may result in electric shock When connecting removing the power plug do so by holding the plug Connecting removing the power plug by holding parts other than the plug may cause fire or electric shock Connect the power plug to a dedicated power outlet with grounding terminals If the power plug is not grounded through ...

Page 41: ...ature setting location Setting range Temperature display range Factory setting of the instrument Remarks 1 Paraffin chamber 50 to 75 C 99 to 99 C 62 C 2 Left warming chamber 50 to 75 C 99 to 99 C 62 C 3 Right warming chamber 50 to 75 C 99 to 99ºC 62 C 4 Hot plate 50 to 75 C 99 to 99 C 62 C A temperature is displayed separately for the left and right but the set temperatures are the same 5 Cooling ...

Page 42: ...he paraffin heating part is indicated by the display color of the temperature display area Temperature control is inactive paused Gray Temperature control is active preparing operating Orange NOTE Each temperature indication is blinking during preparation An error is present Red ...

Page 43: ...fter which the display returns to the Operation screen Touch the button and the display will return to the Operation screen without the setting being saved NOTE The current temperature displayed on the Operation screen will blink until it falls within 3 1 C of the set temperature CAUTION Set the temperatures of the heating parts of the instrument so that the tissues paraffin cassettes and base mol...

Page 44: ...urrent date time At the top the Y field indicates year M field indicates month and D field indicates day At the bottom hours and minutes are displayed If the 12 hour display mode is set AM or PM is displayed Touching the button saves the setting after which the display returns to the Maintenance Menu screen Touch the button and the display will return to the Maintenance Menu screen without the set...

Page 45: ...ration screen 2 Touch the Auto button 2 on the Main Menu screen 3 The Auto Operation Display screen is displayed The start and end times of operation on Sunday through Saturday are displayed Touch on the screen to switch between the display of the first half of the week and the second half of the week 4 Auto operation can be set separately for each day of the week A on the left side of the day of ...

Page 46: ... To set another day of the week repeat the steps from 3 7 To turn on and off the Cryo Module automatically in conjunction with the Embedding Module in the auto operation mode touch the Auto Cryo button to check the Auto Cryo check box 8 When all days of the week and corresponding times have been set touch the button on the Auto Operation Display screen to start auto operation Touching the button r...

Page 47: ...on the Main Menu screen 3 Touch the Language Settings button 3 on the Maintenance Menu screen 4 Touch and select the language to be displayed on the Language Settings screen When a language is selected changes to Touching the button saves the setting after which the display returns to the Maintenance Menu screen Touch the button and the display will return to the Maintenance Menu screen without th...

Page 48: ...n the Main Menu screen 3 Touch the Auto Panel Display Off button 3 on the Maintenance Menu screen 4 If you do not want the panel display to turn off automatically select Not In Use If you want the panel display to turn off automatically you can preset the panel display switch off timer The timer can be set to one of the following 1 minute 10 minutes 30 minutes 1 hour 2 hours When a timer setting i...

Page 49: ...ure of the paraffin warming part has virtually reached the set value When the paraffin heating part starts operating paraffin dispensing is enabled and the work light can be turned on Except in the cleaning mode NOTE Each temperature indication is blinking during preparation even when it has reached the set temperature Details of the Manual Operation Mode Embedding Module The user touches the Heat...

Page 50: ...sed Instrument state TEC 6 EM Active Preparing Operating Paused 4 hours Example of special operation in auto operation If a time earlier than start time 1 is set to end time 1 operation will continue after the 24 00 hour 0 hour and end at end time 1 on the following day Depending on the combination of the start time and the end time on the following day operation may continue after end time 1 Prep...

Page 51: ...the figure below operation will end at End time 1 Paused 24 hours Auto Extension Operation in Auto Operation Touching the Extend button during auto operation turns the extension mode ON and the Extend button active Note that the operation mode differs depending when the button is touched The Extend button is touched while the instrument is active operating The end time of the day will be invalid a...

Page 52: ...Touch the Extend button again to extend the operation continuously till the end time for the next day and later End Operating Operating Paused Resumed operation Paused Preparing 0 AM 0 AM Day 1 Day 2 Display of the Extend button Prep 4 hrs Instrument state Start Time 1 End time 1 End Paused End time 2 Start Time 2 Extend button Stop button Inactive Although the Extend button is pressed the display...

Page 53: ...2 Installation 53 When the End button is touched during auto operation to end the auto operation When next start time has come The extension mode will be OFF but the operation continues ...

Page 54: ...3 Explanation of Operations ...

Page 55: ... the preset start end times Preparations before Use When the instrument is used for the first time or after the instrument has been cleaned paraffin must be added into the instrument and melted If the necessary quantity of molten paraffin is already available proceed to the embedding process Approx 2 5 kg in Paraffin chamber 1 Turn on the respective power switch on the Embedding Module and Cryo Mo...

Page 56: ... paraffin into the paraffin chamber CAUTION Either solidified or molten paraffin may be added into the paraffin chamber or warming chamber but never add paraffin with temperature exceeding 80 C because it will lead to instrument failure Adding solidified paraffin is recommended in order to prevent burns and dripping of paraffin 5 Slide the lid of the warming chamber to the rear of the instrument 1...

Page 57: ... optional small tray is used the two small trays need to be placed in one warming chamber Placing only the one small tray may cause thermal conduction to slow 7 It is posiible to use paraffin in the warming chamber tary to soak cassettes containing tissues Add paraffin to the graduation line marked on the warming chamber tray The tray containing paraffin is called the Tissue Tray 目盛り線 まで入れる 検体トレイ ...

Page 58: ...late and heat the tampers タンパー 使用する際 固まった パラフィ ンが付着しない ようにホッ トプレートで 加温します ホッ トプレート 11 The embedding process can be started once the paraffin has melted in the paraffin chamber and warming chamber and the paraffin chamber temperature indicators change from a blinking light to a steady light Tampers Heat the tampers on the hot plate so that solid paraffin will not attach to the tampers when they are...

Page 59: ...uch as after switching the operation mode to manual once only a small amount of paraffin near the wall of the paraffin chamber may be molten In this case only a small amount of tissues can be embedded 2 Put cassettes containing tissues onto the tissue tray CAUTION In the figure above the tissue basket that comes with Sakura Finetek s Vacuum Infiltration Processor VIP is directly put into the tissu...

Page 60: ... finger to dispense a small amount of paraffin onto the base mold NOTE To make the area around the finger plate brighter touch the Light button on the Operation screen to turn on the work light If the optional foot pedal is connected paraffin can be dispensed by stepping on the foot pedal instead of pressing the finger plate ホッ トプレート Hot plate フィ ンガープレート 底から 1 2mm の パラフィ ン Finger plate 1 to 2 mm o...

Page 61: ...sue with a tamper to fix its position Cool the bottom of the base mold until a thin layer of solidified paraffin is formed 9 Place the cassette on the base mold with the tissue side up 10 Press the finger plate and add paraffin to around 80 of the depth of the cassette コールドスポッ ト タンパー Tamper Cold spot カセッ トの深さの 8 分目程度までパラフィ ンを入れる Add paraffin to around 80 of the depth of the cassette ...

Page 62: ... the paraffin has solidified remove the paraffin block from the base mold 13 Clean the tamper and the interior surface of the base mold using a lint free paper If the interior surface of the base mold is dirty the paraffin block may not be removed easily in the next process If there are still tissues to be embedded repeat the steps from 3 完成したパラフィ ンブロック Completed paraffin block 包埋皿の内表面 赤色の部分 Inter...

Page 63: ...mperature exceeding 80 C into the paraffin chamber or warming chamber because it will lead to instrument failure Also add solidified paraffin in order to prevent burns and dripping of paraffin 3 Close the lids of the paraffin chamber and right left warming chambers Clean each component after use For details on the cleaning refer to 5 2 Cleaning 4 If manual operation was performed turn off the powe...

Page 64: ...e instrument operates according to different settings are explained Setting the Order of Year Month Date and Time Mode 12 24 Set the order in which year month and date are displayed on the screen and whether to display time in the 12 hour mode or 24 hour mode 1 Touch the Main Menu button 1 on the Operation screen 2 Touch the Maintenance button 2 on the Main Menu screen 3 Touch the Date Time Settin...

Page 65: ...on the selected order dates are displayed as follows yyyy mm dd Year Month Day mm dd yyyy Month Day Year dd mm yyyy Day Month Year To display time in the 24 hour mode select 24H To display time in the 12 hour mode accompanied by AM PM select 12H 5 Touching the button saves the setting after which the display returns to the Maintenance Menu screen Touch the button and the display will return to the...

Page 66: ...is not displayed u n l e s s t h e i n s t r u m e n t i s operating 3 Return to the Operation screen 5 C illuminates at the top of the screen and the temperature rises When quick heating ends after 4 hours the previous set temperature is restored for temperature control To stop or cancel the quick heating in progress touch the Quick Heating button again CAUTION Dispensing is disabled during quick...

Page 67: ... using the paraffin flow rate adjustment dial Continue turning the dial and it will come off The dial need not be removed in normal conditions but if it was removed be careful not to lose the spring inside the dial CAUTION Do not close the paraffin flow rate adjustment dial of the Embedding Module fully after the process If the paraffin cools down and solidifies when the flow rate adjustment dial ...

Page 68: ... can be dispensed Adjusting the Brightness Touch the Light button on the Operation screen to turn on the work light and the Brightness Setting button 1 will appear below the Light button The brightness increases by one level every time the Brightness Setting button is touched There are five brightness levels and once the brightness is increased to the highest level the next touch of the button bri...

Page 69: ...dding process by following the steps below 1 Touch the Extend button 1 on the Operation screen 2 The displayed end time temporarily changes to the next end time 2 3 Continue the embedding process 4 When the process is over and you want to pause the instrument touch the End button 3 NOTE When the Extend button 4 is touched again without touching the End button 3 the instrument will go back to the u...

Page 70: ... not yet the end time In this case follow the steps below to end the operation 1 Touch the End button 1 on the Operation screen 2 To end the operation touch the button To cancel the operation touch the button NOTE Once the instrument stops it will remain in the paused mode until the next start time NOTE The sample screen on the right shows the instrument in the paused mode 1 ...

Page 71: ...r side of the wrist rest at the groove between the hand rest and the hot plate 1 and push the wrist rest into a recess attachment point on the front side 2 CAUTION To remove the wrist rest carefully push it up from the recess on the front side using the scraper etc to avoid injuring your fingertip 3 3 2 Wrist Rest Cushion A c u s h i o n c a n b e attached on the hand rest or wrist rest The cushio...

Page 72: ...is pressed Insert the connector of the foot pedal to the connector on the rear of the Embedding Module 1 and turn the screw 2 to secure the connectors It should be noted that the foot pedal cord can be guided between the Embedding Module and the Cryo Module Foot pedal connector 1 Insert 2 Turn 1 2 Foot pedal ...

Page 73: ...it Keep the screw removed from the dispenser separately so that you can easily identify it as the screw for installing the magnifying lens 2 Using the provided wrench turn the lens arm while holding down the dispenser cover to install the magnifying lens WARNINGS Do not remove the dispenser cover If this instruction is not followed you may suffer a burn and or the instrument may fail When the opti...

Page 74: ...4 Error Log ...

Page 75: ...h the Error Log button 3 on the Maintenance Menu screen 4 Errors occurred in the past are displayed each with the date and time of occurrence and the corresponding error code Touch the buttons to navigate through the display pages Up to 40 errors are displayed When errors exceed 40 the errors are deleted from the oldest Touch the button to return to the Maintenance Menu screen NOTE For details on ...

Page 76: ...5 Troubleshooting ...

Page 77: ...tton Temperature control resumes Paraffin in the paraffin chamber is not molten Ambient temperature is too low Use the instrument at an ambient temperature within 10ºC to 35ºC or raise the set temperature of the paraffin chamber The paraffin chamber lid is open Close the paraffin chamber lid The set temperature of the paraffin chamber is too low Raise the set temperature of the paraffin chamber Hi...

Page 78: ...ing chamber The large warming chamber tray was replaced with the small one Place the two small trays in one warming chamber when the optional small tray is used Placing only the one small tray may cause thermal conduction to slow The instrument is exposed directly to the air blowing from an air conditioner Adjust an air flow direction of the air conditioner High melting point paraffin is used in t...

Page 79: ...splay at a time Air bubbles are often trapped in dispensed paraffin The air is present in the paraffin flow line Turn the flow adjustment dial counterclockwise 3 times and half Keep paraffin running for 2 3 seconds to expel the air After the air has been removed put the dial back to its original setting A small lump of paraffin is dispensed A droplet of paraffin is sticking to the tip of the dispe...

Page 80: ...ssage window is still displayed the new message is put on hold After closing the current message window the message window on hold will be displayed The message window shows an error code and a message Touching the button stops the buzzer notification Touching the button closes the message window Caution message Warning message Structure of Error Code E 0 0 0 0 0 Type of error Additional code NOTE...

Page 81: ...affin chamber paraffin line dispenser or dispensing solenoid valve paraffin cannot be dispensed If the problem location is the left right warming chamber or left right hot plate normal operation continues Wait until the temperature of the applicable location displayed on the Operation screen stops blinking and changes to a steady light Wait for a while and if the temperature does not stop blinking...

Page 82: ...e of the Cryo Module If the problem persists contact the Sakura instrument distributor or representative E021 The cooling fan of the Cryo Module does not stop E022 T h e c o o l i n g p l a t e temperature of the Cryo Module does not come down The CM switches to the Paused state The EM continues to operate normally Check the condition of p owe r c o n n e c t i o n a n d power switch state of the ...

Page 83: ... n o t b e r e c e i v e d f r o m t h e temperature sensor Operation aborts Turn off the power to the instrument and contact t h e S a k u r a i n s t r u m e n t distributor or representative E101 E102 A n a b n o r m a l s i g n a l was received from the temperature sensor Operation aborts Turn off the power to the instrument When paraffin in the problem location starts solidifying below 60ºC a...

Page 84: ...ower was turned on Approx 15 seconds later Stops automatically Start notification Short beep The TEC 6 EM is operating Stops automatically End notification Short beep Auto operation has ended Stops automatically Dispensing notification 3 short beeps An attempt was made to perform dispensing operation when dispensing was not possible Stops automatically Paraffin solidified notification 15 second co...

Page 85: ...k if the paraffin inside the paraffin chamber and tissue tray is molten Paraffin is molten You can perform work Paraffin is not molten Wait for paraffin to be molten NOTE To start work quickly touch the Quick Heating button For details refer to 3 2 2 Melting Paraffin Quickly 5 1 4 What to Do When the Low Battery Alarm Icon is Indicated The low battery alarm icon comes on when the voltage of the ba...

Page 86: ...n the finger plate On condition Clean the paraffin drain tray After every use Clean the paraffin chamber On condition Clean the forceps holders On condition Clean the wrist rest cushions On condition Clean the hot plates and cold spot On condition Clean the display screen On condition To clean each part follow the instructions described in this section Use the scraper to scrape off or use a lint f...

Page 87: ...C 3 Wipe down the tray using a lint free paper Cleaning the Paraffin Drain Tray Discard the paraffin in the paraffin drain trays every time after use Before switching off the Embedding Module clean the paraffin drain tray while the paraffin is warm and soft CAUTION Continuing to use the paraffin drain trays without cleaning may cause them to overflow and dirty the surrounding areas 1 Remove the pa...

Page 88: ...e by the scraper forceps etc CAUTION When paraffin dirties the periphery of the tray it sticks to the inside of the instrument or the bench and may become hard to pull out When cleaning the inside of the instrument there is a risk of scattering the paraffin or getting burned by heated rear cover underneath the hot plate Wear rubber gloves and protective eye gear during cleaning 4 Insert the paraff...

Page 89: ...leaning Mode button 2 on the Main Menu screen and the display will switch to the cleaning mode screen and the cleaning mode will start During the cleaning mode the heaters for the forceps holders and hot plate stop and the temperatures drop On this screen the current temperature of the forceps holders is displayed 3 When the forceps holder temperature reaches 50ºC the Heat button 3 appears Then wh...

Page 90: ...e Heated message and the alarm is user selectable Tap the temperature area 4 on the screen to display the setting window and change to a desired value 6 Remove the paraffin from each forceps well using forceps 7 Wipe down the interior of each forceps well using a lint free paper or cotton swab to remove foreign matters 8 After the cleaning is finished touch the button 5 to end the Cleaning mode 4 ...

Page 91: ...y Cleaning the Hot Plates and Cold Spot 1 Remove paraffin attached to the hot plate and cold spot using the scraper wrapped with a lint free paper 2 For paraffin or foreign matters in the grooves of the hot plate remove it using cotton swabs CAUTION The hot plates are very hot Wear rubber gloves or other protective gears to prevent burns Finger plate Scraper wrapped with a lint free paper Cold spo...

Page 92: ...event burns 1 Turn on the Embedding Module power switch 2 If the instrument is paused touch the Heat button 1 to activate the instrument If the instrument is in the auto operation mode touch the Extend button 2 to activate the instrument 3 Check if the paraffin inside the paraffin chamber is molten If not wait for the paraffin to be molten or touch the Quick Heating button Refer to 3 2 2 Melting P...

Page 93: ...ne between the paraffin chamber and the dispenser the internal parts may degrade or deform which will cause an instrument failure 6 Remove the strainer and wash it with warm water approx 70 ºC 7 Completely wipe off any water droplets and return the strainer into the paraffin chamber Cleaning the wrist rest cushions Remove paraffin attached to the cushions and rinse with warm water at 70 C or below...

Page 94: ... to lock the display 2 Gently remove paraffin attached to the screen surface using the scraper wrapped with a lint free paper 3 Hold down the Lock Display button again to unlock the display NOTE To order the screen protection sheet contact the Sakura instrument distributor or representative 1 ...

Page 95: ...5 Troubleshooting 95 ...

Page 96: ...6 Replacement Parts ...

Page 97: ...5123 1 1 1 1 1 Wrist rest cushion L 5124 1 1 1 1 1 Screen protection sheet 5125 1 1 1 1 1 Power cord A4010541 1 A4010537 1 A4010534 1 1 1 6 2 Service Life and Maintenance The service life of the instrument is 8 years after shipment from manufacturer Conditions Follow the precautions for use and periodic maintenance procedures described in this operating manual Replace the following major component...

Page 98: ...7 Glossary of Terms ...

Page 99: ... to help position the tissue Control panel display Used for running stopping the instrument and setting various parameters and conditions It also serves as a monitor that displays the operating status of the instrument Cooling plate A cooling device on the Cryo Module side It is a cooling plate that cools solidifies the embedded paraffin block Dispenser A dispenser that dispenses paraffin used for...

Page 100: ...embedding and equipped with a heater The paraffin chamber also has a lid to keep dust out etc and temperature drop There is a mesh filter at the bottom of the chamber to filter out dust from the paraffin to be dispensed The capacity is 4 L of paraffin in molten state Graduations are provided on the interior of the chamber for reading the remaining level Paraffin drain tray A tray for collecting th...

Page 101: ...work flow Warming chamber tray A tray set in a warming chamber It is used as either a base mold tray or tissue tray depending on the purpose A large tray can accommodate one 150 cassette basket or two 75 cassette baskets of the Vacuum Infiltration Processor VIP series The optional small tray can accommodate one 75 cassette basket Work light A LED light that illuminates the work surface Wrist rest ...

Page 102: ...102 ...

Page 103: ...M01 021E 02 ...

Page 104: ...Please visit our websites www sakura finetek com www sakuraus com www sakura eu ...

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