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PippinHT Operations Manual
iii
460005 Rev G
TABLE OF CONTENTS
1 INTRODUCTION
…………………………………………………………..……………………..……….1-1
1.1 System Overview ....................................................................................................................... 1-1
1.2 How the system works .............................................................................................................. 1-2
1.3 Collection Strategies ................................................................................................................. 1-3
1.4 Collection Types ........................................................................................................................ 1-4
2
KEYS TO SUCCESSFUL COLLECTIONS ...................................................................... 2-1
2.1 Common Misconceptions ........................................................................................................ 2-1
2.2 Improving Sample Yield ........................................................................................................... 2-3
2.3 Improving Sample Yield for HMW DNA (High-Pass
Protocols) ............................................ 2-3
3
SAFETY AND PRECAUTIONS ....................................................................................... 3-1
4
UNPACKING AND INSTALLATION ................................................................................ 4-1
4.1 Unpacking the PippinHT ........................................................................................................... 4-1
4.2 Setting up the PippinHT ............................................................................................................ 4-1
4.3 Unpacking Gel Cassette Kits .................................................................................................... 4-3
4.3.1 Storage Conditions for Cassettes and Reagents................................................................ 4-4
5
WORKFLOW SUMMARY ................................................................................................ 5-5
6
SAMPLE PREPARATION ............................................................................................... 6-1
6.1 Input Sample Characteristics .................................................................................................. 6-1
6.2 Preparing DNA Samples for the PippinHT ............................................................................. 6-1
6.2.1 Sample preparation for use with an internal standard ....................................................... 6-1
6.2.2 Sample preparation for use with an external marker ........................................................ 6-2
6.3 Recommended sample Load Guidelines ................................................................................. 6-2
7
PROGRAMMING A PROTOCOL .................................................................................... 7-1
7.1 Overview .................................................................................................................................... 7-1
7.1.1 Protocol Parameters Detail ................................................................................................ 7-2
7.2 Writing a Size Selection Protocol ......................................................................................... 7-4
7.3 Using an External Marker ....................................................................................................... 7-9
