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PippinHT Operations Manual
7-2
460005 Rev G
7.1.1 Protocol Parameters Detail
The protocol parameters section is shown below. Size selection must are entered for
lane pairs and different values may be entered for each lane pair. The odd-numbered
lanes are the lanes which are optically detected.
The function of the columns or fields are provided below. From left-to-right:
Programming Mode
s
The user selects which of the three size selection modes that will be used. When this is
selected, the editable fields will appear in the size selection parameters:
•
Tight
- collects the narrowest fragment distribution that can be collected by the
system.
The user enters a target base-pair value (the median for the distribution) in the size
selection parameters.
•
Range
– collects a fragment range. The user enters a start and end base-pair
value in the size selection parameters.
Note:
Range mode is used for programming High-Pass protocols.
•
Time
–
collects fragments base on a starting elution time (time after the
beginning of a run) and ending elution time.
N
ote:
Programming modes may be independently applied to individual sample lanes
Programming
Modes
Size selection
parameters
(in base pairs)
Time-mode
parameters
Cassette 2 Tab
