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Incubator

User Manual

Revision B

March 2007

Summary of Contents for 170-200P

Page 1: ...CO2 Incubator User Manual Revision B March 2007 ...

Page 2: ...otech Laboratory Equipment Ltd All Rights Reserved Reproduction adaptation or translation without prior written permission from RS Biotech is prohibited Disclaimer Notice RS Biotech Laboratory Equipment Ltd reserves the right to change information in this document without notice Updates to information in this document reflect our commitment to continuing product development and improvement ...

Page 3: ...tions NOTE Notes contain essential information that deserves special attention CAUTION Caution messages appear before procedures which if caution is not observed could result in damage to the equipment WARNING Warning messages alert you to specific procedures or practices which if not followed correctly could result in serious personal injury Bold Text in boldface type emphasises key words or phra...

Page 4: ...re Alarms 19 3 5 2 Setting the CO2 High Low Alarms 20 3 5 3 Door Open Alarm 20 3 5 4 Alarm Duration 20 3 5 5 Alarm Arming Delay 21 3 6 CHAMBER ALARM SYSTEM 21 3 6 1 Temperature Sensor System Alarms 23 3 6 2 Over Temperature Cut out Alarm 23 3 7 CO2 AUTO ZERO SYSTEM 24 4 ROUTINE MAINTENANCE 26 4 1 GENERAL NOTES 26 4 2 DAILY CHECKS 26 4 3 WEEKLY CHECKS 26 4 4 MONTHLY CHECKS 26 5 SERVICE 27 5 1 FUSE ...

Page 5: ...5 RS Biotech User Guide 8 4 OTHER OPTIONS 39 8 5 COPPER INNER CHAMBER 40 ACCESSORIES 41 8 6 AVAILABLE ACCESSORIES 41 9 CE CERTIFICATION 42 10 INDEX 43 ...

Page 6: ...olished stainless steel The non tip shelves shelf racks and humidity tray are easily removed without tools for thorough cleaning and are capable of being sterilised Air circulation is achieved without the use of a fan eliminating ductwork a potential source of contamination simplifying cleaning eliminating vibration and reducing small sample evaporation within the chamber The incubator incorporate...

Page 7: ...er s Guide for instruction and reference To simplify lifting the incubator leave it on the pallet You must have at least one person at either side to safely lift the unit Supported by the base only remove the incubator by lifting it from the delivery pallet NOTE Remove the transportation bracket from the front underside of the incubator at the opposite side to the hinge side Locate and remove the ...

Page 8: ...umidity Tray 4 Adjustable Feet with locking nuts Packed in Humidity Tray 4 Anti slip Pads for adjustable feet Packed in Humidity Tray 1 User Manual Supplied 2 3 Utilities In order to use the incubator you will need Table 2 Utilities Utility Requirement 110 120V 50 60 Hz grounded electrical supply with min capacity of 8 amps 10A if High Temperature Decontamination Option is fitted OR Electricity 22...

Page 9: ...ately 75 kg actual use weight will be heavier and will depend on the options installed and the material stored in the incubator The incubator is designed so that one incubator can be safely stacked on top of another identical unit using the stacking kit order code 170 147P It is not possible to put any other type of incubator or heavy apparatus on top as the top cover and stacking kit is designed ...

Page 10: ... racks and shelves and level the incubator 1 Oriented as shown in Figure 1 on the following page place the shelf racks inside the chamber Each rack has three rubber feet two stand on the floor of the chamber and one stands against the back wall Make sure the cushioned tubing spacers are snug against the side walls these spacers allow clearance for the shelves Figure 1 Inserting Shelf Racks ...

Page 11: ...all the tie rod at the back of the shelves to hold both sides together as shown in Figures 2 3 You may prefer to do this prior to inserting the racks in the chamber Figure 2 Fitting Tie Bar to Shelf Racks Figure 3 Shelf Rack Assembly ...

Page 12: ...roperly inserted onto each of the shelf rack guides Figure 4 Inserting Shelves as seen from the back 4 Level the incubator by adjusting the feet Place a small level on the second shelf of the incubator Adjust the levelling feet until the incubator is level and stable Lock the levelling feet in place by tightening the locking nuts on each foot ...

Page 13: ...5 Slide the humidity tray onto the lowest shelf rack supports which are shaped with stops to keep the tray in place see Figures 5 6 Figure 5 Installing the Humidity Tray Figure 6 Humidity Tray Installed Equipment Tray left side ...

Page 14: ... filter by attaching the tubing from the two stage regulator or in line regulator to the matching CO2 inlet on right side of the equipment tray see Figure 7 Figure 7 Making Connections 2 Use the tubing clips provided to eliminate CO2 leaks ON OFF Switch CO2 Inlet Fuse Holder CO2 Sampling Port Label Power Cord Receptacle REAR PANEL of incubator RIGHT SIDE of Equipment Tray ...

Page 15: ... connections verify that your power supply voltage matches the voltage of your unit and that the on off switch is in the OFF position Install the power cord 1 Insert the power cord into its receptacle on the right side facing the unit of the equipment tray see Figure 7 on the previous page 2 After verifying that your supply voltage matches the voltage specified for your incubator and that the ON O...

Page 16: ...wn through numbered values in the display Press this key simultaneously with the Enter key to access the Alarm system Up In Programming mode use this key to scroll up through numbered values in the display Enter Press this key to save a new set point Press this key simultaneously with the Down key to access the Alarm system NOTE If you accidentally press both the Up and Down keys simultaneously yo...

Page 17: ...unded power supply 5 Turn on the incubator the display will illuminate Humidifying begins when the temperature reaches the programmed level NOTE If the power receptacle has a switch make certain the power receptacle is switched on When the incubator turns on the decimal points on the display for Temperature and CO2 flash on and off to signify that the Alarm System is not armed 6 Turn on the gas su...

Page 18: ...e shown that in addition to inhibiting bacterial growth in the tray this can reduce contamination on the chamber walls 3 For IVF and other sensitive work we do not recommend the use of any biocide in the humidity tray To reduce the possibility of contamination every 10 14 days the humidity tray water should be changed and the humidity tray should be cleaned with a solution of 70 isopropyl alcohol ...

Page 19: ...ing key to work in the CO2 display then press the Enter key NOTE Allow the incubator to stabilise at the set points selected before continuing 3 5 Programming the Alarm System 3 5 1 Setting High Low Temperature Alarms 1 Press the keys simultaneously to enter Alarm Program Mode The display will show C AL 2 Press the key to display the High Temperature Alarm HI37 5 The factory setting is the setpoin...

Page 20: ...en you open the door an alarm will sound after a preset time delay If you wish to adjust the time delay 1 In Alarm Program Mode see Step 1 of Section 3 5 1 above press the key until the display shows doo rAL reading across both displays 2 Press the key and the keys to adjust the time as you scroll through the available choices you will see 15 30 45 60 75 90 seconds then OFF 3 Press to save the des...

Page 21: ...t point values 0 1 are achieved after which the Alarm System is armed See Figure 10 Chamber Alarm System Flow Chart on the following page If temperature and or CO2 levels deviate more than the programmed amount the display flashes the audible alarm sounds and a message appears on the screen You can acknowledge and cancel the alarm by pressing any key When the door is opened the Alarm System is dis...

Page 22: ... DOOR DISARM ALARMS CLOSE INNER DOOR 15 MINUTES ALARM DELAY TIMEOUT STARTED IS TEMPERATURE OR CO2 LEVEL MORE THAN 0 5 OUTSIDE PROGRAMMED POINTS CANCEL ALARM BY PRESSING ANY KEY ALARMS CANCELLED VISUAL AND AUDIBLE ALARMS CANCELLED MESSAGE LEFT ON SCREEN CANCEL MESSAGE BY PRESSING ANY KEY NO YES YES SWITCH ON ARM ALARMS VISIBLE AND AUDIBLE ALARM MESSAGE ON SCREEN HAVE CONDITIONS RECOVERED TO WITHIN ...

Page 23: ...tion the over temperature cut out and alarm system operates in two sequential modes Mode 1 The over temperature cut out and alarm activates and cuts power to the heating elements and the CO2 control valve The condition is shown on the display by the message Ct riP When the chamber temperature has fallen to the programmed set point the system changes to Mode 2 Mode 2 The control system then tries t...

Page 24: ...mp switches off and chamber atmosphere moves back into the measuring chamber of the sensor This takes 3 minutes after which the normal CO2 control takes over On completion of the auto zero a prompt will appear CO2 Aut O ZEr O In rAn gE NB Press the key to finish the cycle and return to the main display If the CO2 Auto Zero cannot reference the signal to atmosphere at the end of the Auto Zero the f...

Page 25: ...curs the auto zero will automatically take place after 1 minute This is to confirm that the CO2 sensor is correctly referenced If after a 15 minute delay the CO2 is still in an alarm condition and re alarms it will again auto zero In the event of high CO2 alarms open the inner door for a few seconds to ensure that the CO2 level drops below set point Also check that the CO2 pressure is set correctl...

Page 26: ... large cylinder should last approximately 12 months If there is a significant drop at the cylinder pressure of 725psi or 50 bar it means that the cylinder is almost empty and should be replaced Ensuring that there are no leaks at any of the connections will ensure a greater lifetime to the CO2 supply and will help avoid accidentally running out of CO2 4 3 Weekly Checks Refill the humidity tray to ...

Page 27: ...ded 5mm maximum screwdriver 1 Using the flat bladed screwdriver rotate the fuse holder see Figure 7 for location anticlockwise until the fuse holder springs out 2 Remove the spent fuse 3 Install a new fuse of the same type 4 Orient the fuse holder back in the side panel and using the screwdriver as before secure it in place ...

Page 28: ... Protect all electrical connections from contact with the alcohol solution To best protect yourself your incubator and your work area follow these instructions 1 Program 0 0 CO2 and switch off the incubator Unplug the unit from the power supply 2 Dampen a clean cloth with the alcohol solution and wipe down all external surfaces taking care to keep the alcohol solution from coming into contact with...

Page 29: ...Wipe the internal components of the chamber twice with the alcohol water solution Wipe off excess liquid and leave it to dry completely 8 Re assemble the shelf racks the humidity tray and the shelves before switching the incubator on 9 Be sure to remove the protective cover from the sensor and put it back in the holder for safekeeping 10 Refill the humidity tray as explained in Section 3 3 11 Leav...

Page 30: ...s Connections 6mm tubing Required Gas Pressure 0 35bar 5psi Relative Humidity Removable stainless steel humidity tray Reservoir Capacity 2 5 litres Humidity Control 95 at 37 C Shelves Polished stainless steel non perforated standard Dimensions WxD 520 x 426mm Number of Shelves 4 standard up to 8 shelves with multiple position option Alarm Systems Two level alarm system giving programmable audio vi...

Page 31: ...ration of the incubator is carried out at 37ºC 5 0 CO2 and 95 RH in an ambient temperature of 20 25ºC with no heat generating apparatus inside the chamber Software calibration adjustments may be required to optimise performance if the incubator is being used well outside these operating conditions Performance specifications may also be affected For advice on calibration adjustments and relevant pe...

Page 32: ...m 170 214P Yes Internal IP66 Mains Socket 170 224P No Below Bench Castor Set two castors 170 148P Yes Key Lock on Outer Door 170 233P No Copper Chamber 170 016P No 8 1 BMS Relay Contact Alarm The BMS Building Management System Relay Contact Alarm allows a signal from a central alarm system to be switched ON or OFF to indicate an alarm condition at the incubator The following alarms would activate ...

Page 33: ... the key until rEL Ay is shown in the display 3 Press the key to select the option 4 The default setting is nO OFF press the key to change the display to yEs ON or press the key to return the display to nO OFF 5 Press the key to accept the selection 6 To return to the temperature and CO2 display screen press the key twice 8 2 High Temperature Decontamination High temperature decontamination is des...

Page 34: ...to chemical decontamination Certain areas of the glass door and inner door seal surface temperatures will be somewhat lower at 115 C 5 C This is the minimum temperature necessary to eliminate Bacillus Subtilis which is the standard organism specified by the US Pharmacopeia to validate sterilisation of chamber interiors It is important that these areas be chemically decontaminated prior to running ...

Page 35: ... indicate which decontamination stage the incubator is in HE At heating to 120 C dEC On maintaining 120 C or Co oL cool down The time displayed counts up during the heat and cool stages but it counts down from 4 hours during the decontamination stage After the cool down phase is completed the following messages will be displayed depending on the outcome dEC ON PAS Sed or dEC ON FAI LEd If the mess...

Page 36: ...nation cycle the process will continue as normal A failure message will however appear if the temperature falls as a result of the door opening during either the heating phase or the decontamination phase If the incubator power is cycled OFF then ON the incubator will power up as normal This condition will be indicated by an absence of a completed decontamination status message either dEC ON PAS S...

Page 37: ...contamination model CAUTION Working with mains power inside a humid environment where the incubator is humidified can be hazardous The following precautions should be observed The instrument or equipment and its external connections to be used inside the chamber should be specified as suitable for use in a humid environment and at 37 C see also Using Powered Equipment within the Chamber Always ens...

Page 38: ...ow heat output for use in incubators The maximum heat that can be dissipated within the chamber is variable dependant on the difference between the ambient and programmed temperatures If the heat dissipated is too great the chamber will tend to overheat If this happens the control parameters can be adjusted to compensate NOTE With an ambient temperature of 23ºC maximum and a programmed temperature...

Page 39: ... and the connecting cable should be removed from the chamber and the Sealing Cap replaced on the socket prior to starting a High Temperature Decontamination cycle Figure 13 IP66 Socket 8 4 Other Options An RS 232 port see Figure 10 for location is available for factory installation or to be retrofitted on site by an authorised service person A Centronics printer port see Figure 10 for location is ...

Page 40: ...er Chamber NOTE Copper naturally changes colour this unit had a bright finish when at manufacture The dull finish is a result of oxidation of the copper surface and it is the properties of this oxidation that creates the important antimicrobial surface ...

Page 41: ...re with RS232 170 117P Galaxylog V1 0 170 131 Galaxy IR CO2 Analyser 010 100 Galaxy IR CO2 O2 Analyser 010 1000 8 Position Shelf Rack pair 170 141P Shelf Perforated 170 142P Shelf Non perforated 170 143P Copper Shelf Standard 170 144P Copper Shelf Perforated 170 145P Stacking kit stand with castors 170 146P Stacking kit no stand 170 147P Below Bench Castors 170 148P Stacking Kit Stand with Feet 17...

Page 42: ...42 Galaxy S CO2 Incubator User Guide 9 9 C CE E C CE ER RT TI IF FI IC CA AT TI IO ON N ...

Page 43: ...tion 42 Chamber Alarm System Functions of 21 Chamber Alarms Flow Chart 22 CO2 High Alarm 20 CO2 Low Alarm 20 CO2 Setpoint Range 19 Condensation 26 Control Panel 16 Copper Inner Chamber 40 Copyright Notice 2 D Daily Checks 26 Direct Heating System 6 Disclaimer Notice 2 Disinfecting 28 Displays 16 Door Open Alarm 20 Down Key 16 E Enter Key 16 F Factory Set Setpoints 18 Function Keys 16 Fuse Replacem...

Page 44: ...t Alarm 23 P Power Cord 8 Printer Port 39 Programming Key 16 PVC Tubing 8 R Rear Castors 39 Recalibration 31 RS 232 Port 39 S Sensor Covers 8 17 Setpoints Factory Set 18 Setting CO2 18 Setting Temperature 18 Setup 10 Setup Concerns Issues to Avoid 9 Shelf Racks 8 Shelves 8 Specifications 30 T Table of Contents 4 Temperature High Alarm 19 Low Alarm 19 Temperature Sensor System Alarms 23 Temperature...

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