XCell
TM
ATF System with C410:V4B Controller
User Guide
52
a.
The ∆P between
PRV1-P2 should be greater than
P-Pressure Offset
and
∆
P
between PRV2-P2 should be less than
E-Pressure Offset
. If not, manually lower
PV in small
increments.
b.
Flow is too rapid- A flow that is too rapid during the Exhaust Cycle can be readily
detected on the P2 trend by a rapid decline in pressure following a stable
pressure profile. (The Pump exhausts too rapidly followed by a rapid pressure
drop). Decrease PV2 or PRV2 to decrease flow as a corrective measure.
•
Select the P2 trend in the MAIN screen and observe P2 profile, The P-Pressure Offset
and the E-Pressure Offset should be apparent.
Any adjustments in P2 trend on the Main screen are performed from the P2 TREND screens
6.5
Examples
XCell™ ATF
System process control settings will depend on the process requirements. Each
user or process may have its own unique requirements. Hopefully, the example provides a
guideline to assist the users in selecting and optimizing operating conditions.
When working with an
XCell™ ATF
System connected to an unpressurized vessel, refer to
Figure 1 for an overview of positioning the Filtration Assembly, P-Box and E-Box relative to
the bioreactor.
Example 1
Using an unpressurized bioreactor with an
XCell™ ATF
6 System:
When using a bioreactor that cannot be pressurized such as a glass vessel or a disposable
vessel, i.e., SUB, the connection between the Filtration Assembly and the SUB will most
likely not be an SIP type connection shown in Figure 1. Placement of the Filtration Assembly
and controller relative to the bioreactor will, however, not change significantly.
Using a vessel that cannot be pressurized, both pressure and vacuum services are needed.
See Utility Requirements, Section 2.
Repligen Corporation offers custom, disposable connections to most commercial SUBs. The
connection procedure between the Filtration Assembly and vessel are provided separately.
Start
1.
Connect the Filtration Assembly to bioreactor per separate instructions.
2.
Place the P-Box in proximity of the Filtration Assembly.
3.
Connect Signal cable between E-Box and P-Box.
4.
Connect E-Box to an appropriate electrical power source
5.
Connect the Air Line, Exhaust Line and Pump Line to their respective ports on the P-
BOX. Do
not connect the Pump Line from the C410v4B controller to the
Diaphragm Pump at this point.
6.
Power ON the E-Box. Wait for system to boot up and display the
XCell™ ATF
System
INITIAL screen.
