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R-Biopharm Rhone Ltd
5
HPLC Conditions
HPLC Conditions
Derivatisation
KOBRA® CELL at 100 µA setting
Guard Cartridge
Inertsil ODS-3
5 µm, 4 mm x 10 mm or (Hichrom) equivalent
Analytical Column
Inertsil ODS-3V
5 µm, 4.6 mm x 150 mm (Hichrom) or equivalent
Mobile Phase
Water : Methanol (60 : 40 v/v)
Add 119 mg of potassium bromide and 350 µl 4 M Nitric Acid to 1 litre of mobile
phase
HPLC Pump
To deliver mobile phase
Flow Rate
1.0 ml/minute
Fluorescence
Detector
Excitation: 362 nm
Emission: 425 nm (B1 and B2)
455 nm (G1 and G2)
Column Heater
Maintain guard and analytical columns at 40 °C
Integrator / Data
Control System
From preferred supplier
Injector
Autosampler / Reodyne valve
Injection Volume
100 µl
Elution Order
G2, G1, B2, B1.
Summary of Contents for KOBRA CELL
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