QwikCheck™
Sperm Quality Analyzer Guide Version 1.00 I-Button WHO 5th 06_AUG_2012
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APPENDIX 12: COUNTING CHAMBERS (Concentration Standard Settings)
A number of commercially available counting chambers are used in laboratories for manually counting
sperm cells. These chambers vary by depth and one type requires a diluted sample. It has been clinically
established that counts vary by approximately 30% depending on the chamber used.
The QwikCheck GOLD HUMAN permits the user to select the type of chamber the laboratory has
implemented as a standard for manual semen analysis. Once the concentration standard (CONC.
STANDARD) has been selected the QwikCheck GOLD will automatically run semen samples based on that
standard.
SQA-V Set-Up:
From the
MAIN MENU
select:
SERVICE > SET-UP > SYSTEM DEFAULTS
to view the screen
below:
Select a
CONC. (concentration) STD (standard) 1 or 2
based on the options shown in the
table below.
Commercially available counting chambers are divided into two unique groups:
1.
Standard #1:
10-20 micron depth and do not require sample dilution.
2.
Standard #2:
100 micron depth (haemocytometers) that require sample dilution.
The table below classifies some commercially available chambers:
CHAMBER STANDARD #1
CHAMBER STANDARD #2
Makler
Beurker-Tuek
Micro-Cell
Buerker
Fixed Cover slip disposable chambers
Fuchs-Rosenthal
Fuchs-Rosenthal (modified)
Improved Neubauer
Neubauer
Malassez
Thoma
Thoma Modified
SYSTEM DEFAULT SETTINGS
DATE FORMAT: MM/DD/YY / DD/MM/YY
DATE/TIME SETTING: 01/04/05 08:15:45
AUTO PRINTING: YES/NO
# LABELS TO PRINT: 1 / 2
CONC. STD: 1 / 2
