QwikCheck™
Sperm Quality Analyzer Guide Version 1.00 I-Button WHO 5th 06_AUG_2012
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APPENDIX 7: Dilution Media
Product Insert
INTRODUCTION AND INTENDED USE
The QwikCheck
TM
Dilution Kit is to be used to dilute semen samples prior to automated/manual testing
and for semen sample preparation. The dilution media is Earle’s balanced salt solution which contains
ingredients to support sperm motility and viability and is recommended by WHO 2010, 5
th
edition, pg.
163 for semen sample preparation. The product is intended for in vitro use only.
KIT CONTENTS
50 ml of sterile Earle’s Balanced Salt solution
Product Insert
STABILITY AND STORAGE CONDITIONS
The product has one-year shelf life. Note the expiration date on the box and bottle.
Store bottle in the refrigerator upon receipt. Bring to room temperature (22-26°C) prior to use.
Avoid prolonged exposure to light.
Do not use if the solution contains precipitate or is cloudy.
INSTRUCTIONS FOR USE:
AUTOMATED SQA-V:
1.
Measure the volume of the neat semen sample.
2.
If the volume is less than 0.5 ml, dilute 1:1
3.
Open the dilution kit bottle and pipette an amount of Earle’s solution that is equal to the semen
sample volume measured in step 1.
4.
Add the Earle’s solution to the neat semen sample and thoroughly mix the sample by rotating the
container in a circular manner. This will evenly distribute the spermatozoa throughout the sample
without introducing bubbles.
5.
Fill the SQA-V testing capillary immediately after the sample is mixed following the SQA-V on-
screen instructions
MANUAL:
Follow laboratory sperm preparation protocols for diluting semen samples for testing.
PRECAUTIONS AND WARNINGS
Exercise appropriate precautions to minimize direct contact with skin or eyes and prevent inhalation.
REFERENCES:
WHO 2010 Laboratory Manual for the Examination of Human Semen, 5
th
Edition, Cambridge University
Press, 2010, pg. 163.
