QIAamplifier 96 User Manual 03/2020
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This manual provides information on how to use the QIAamplifier 96 Thermal Cycler most
effectively.
4.1
High speed thermoblock
All blocks of the QIAamplifier 96 thermal cycler are made of aluminum and offer high ramp rates
for fast protocol run times. A rubber seal on the heated lid forms an encapsulated space around
the sample block as soon as the lid is closed. The closed space serves to improve the temperature
uniformity of the sample block and avoids the formation of condensed water at the final PCR cooling
step. Furthermore, all blocks are perfectly sealed to prevent condensed water from penetrating into
the Peltier elements underneath the sample block and other parts of the electronics. The sealing
protects the Peltier elements and prolongs the lifetime of the device.
4.2
High Performance Smart Lid
The QIAamplifier 96 heated lid has been optimized to shorten protocol run times. Moreover, the
heated lid fulfills two other important functions: It prevents the formation of condensation at the
reaction tube portion located above the block surface level, and it ensures reliable contact between
the reaction tubes and the thermoblock by applying constant pressure. Thanks to the integrated
clutch mechanism, the pressure applied to the reaction tubes is always the same, regardless of the
plasticware height. The combination of the heated lid shape and the reliable contact pressure
ensures even temperature distribution between samples, thus significantly improving temperature
uniformity.
With one press on the front button, the heated lid gently swings open and locks in its end position.
This mechanism ensures that the lid cannot fall and the heated lid is out of the radius of action while
the user inserts PCR tubes or plates. This prevents any risk of injury from crushing or hot surfaces.
4.3
Display
The QIAamplifier 96 thermal cycler features a 7” state-of-the-art color touchscreen user interface.
The touchscreen is built-in at a flat angle to ensure reflection-free viewing and ergonomic
programming. For programming PCR protocols, the software incorporates QIAGEN spreadsheet
and alternatively offers graphical programming. One touch of a button leads from the spreadsheet
to the alternative graphical programming mode. This makes the creation of new programs or editing
existing programs fast and easy.
