Qiagen DML 3000, User Manual, Page: 15 / 81

Functional Description
DML 3000 User Manual 3-1
3 Functional Description
The DML 3000 is highly sensitive with a broad dynamic range. It features a low
background photon-counting PMT and measures light in the visible spectrum (350–
650 nm).
Microplates are loaded at the front of the DML 3000. The individual microplate wells
are sequentially moved beneath the opening of the detector and then individually
measured. A step motor moves the microplate in the Y-direction to present the
microplate wells to the detector. A separate stepper motor moves in the X-direction
over the microplate.
An internal microprocessor is used to control the basic functions and communications
with the HC2 System PC via a standard RS-232 serial port.
The performance specifications of the DML 3000 are in the following table.
Performance measure Specification
Spectral sensitivity range 350–650 nm
Dynamic range 10 to 5x10
6
RLU
Cross-talk Less than 4.0 x 10
–5
RLU
The most important physical parameters for measuring a microplate are:
Reflectivity of the light spectrum measured at the detector
Absorbency of the light between adjacent microplate wells
Homogeneity and reproducibility of the parameters of the detector
Physical dimensions and properties, such as the flatness of the surface
Accuracy and reproducibility of all physical dimensions