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THE THEORY OF FOCUSING
Select the Live Capture tab:
HoloMonitor has both a mechanical and a software focus. They need to cooperate in order for
the image to be in focus. With the mechanical focus, the user moves the cells to the approxi-
mately correct distance from the objective. The software focus then finds and calculates the
cell images. The
Software focus panel
indicates how well the computer is able to focus an
image, based on the light information that reaches the sensor. When the focus bar is in the
middle of the green field, the focus is optimal.
The software focuses the images only when the cell culture vessel is at approximately the
correct distance from the objective. The aim is to have the cell surface of the cell culture
vessel approximately 1 mm above the object tip.