PerkinElmer OPERETTA, Technical Manual

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Fluorescence emission and transmitted light from the sample is collected through
bandpass filters on a emission filter wheel and detected by a high sensitivity Peltier
cooled CCD camera. Special optics enable viewing of an enlarged field of view /
number of cells in an image view without compromising optical resolution.
The Operetta unit contains all necessary hardware and software for four color
measurements (UV, green, orange and far red) using a 20x long WD objective – see
below for the accessories included. Briefly they comprise the instrument with four
excitation filters, four dichroics, four emission filters, 20x objectives, camera, Xenon
lamp, laser based auto focus and one set of Harmony Software included in the
instrument.
Please see the following table for an overview of standard and optional components of
the optical setup such as lenses, filters, dichroic mirrors etc.
Component Standard Configuration Optional Configurations
Objective lens 20x long WD 2x long WD
10x long WD
10x high NA
20x high NA
40x long WD
40x high NA
60x long WD
60x high NA
100x long WD
Excitation filter 380/40 (UV)
475/30 (Green)
535/30 (Yellow)
630/20 (Far Red) 420/20
500/20
570/20
615/30
Dichroic mirror 405
435
495
515
555
585
635
645
Emission bandpass filter 445/70
525/50
595/70
705/110
5 Technical Data
Operetta Technical Manual 57