PerkinElmer OPERETTA, Technical Manual

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Caution!
Sharp-edged sample carrier holder!
l
Be careful when performing retooling or maintenance tasks on
the scanning table and the objective lens changer.
l
Do not touch the sample carrier holders.
l
Switch off the Operetta before working on the scanning table,
so that the table is in the service position.
3. Objective lens changer
The objective lens changer automatically changes between up to 4 installed
lenses via software control. The selected lens is moved to measuring position.
Available objectives: 2x, 10x, 20x, 40x, 60x, 100x (long WD and high NA)
4. Transmission light source for brightfield excitation
o
Detect cells independent of dye, label-free techniques
o
740nm Infrared to minimize cell damaging
o
Extension arm with integrated transmission light source (has to be moved
into measuring position above the objective before starting brightfield
measurements)
o
Maintenance-free LED
o
For brightfield measurments in combination with climatization see chapter
2.3.2 "Live Cell Chamber", page 28.
2.2.6 CCD Camera
The Operetta uses one high resolution 14 bit CCD camera for cell imaging.
The camera is cooled down (20 °C offset) by a Peltier element.
2 Hardware Description
26 Operetta Technical Manual