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PerkinElmer OPERETTA, Technical Manual

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O

PERETTA

®

Technical Manual

Summary of Contents for OPERETTA

Page 1: ...OPERETTA Technical Manual...

Page 2: ...or translated into any language without the prior written permission of PerkinElmer Inc Copyright 2011 PerkinElmer Inc Printed in the UK Trademarks Registered names trademarks etc used in this documen...

Page 3: ...nterference 14 1 6 Warranty Information 14 1 7 Conformity 16 1 8 Service Schedule 17 1 9 Setup Requirements 17 2 Hardware Description 19 2 1 Introduction 20 2 1 1 Instrument Features 20 2 2 Operetta D...

Page 4: ...equirements for Harmony Installation on an Office PC 51 4 4 Computer Systems 52 4 4 1 Network 52 4 4 2 Hardware 52 4 4 3 Operating System Software Software Applications 52 4 4 4 Data 53 4 4 5 Back Up...

Page 5: ...1 System Safety 1 System Safety Operetta Technical Manual 5...

Page 6: ...r for usage in biological medical and pre clinical research biosafety levels 1 and 2 see also section 1 3 3 Process Liquids or Substances page 9 Due to its technical design the Operetta is particularl...

Page 7: ...inor injury Notice Failure to observe may result in invalid measurement results or damage of the instrument Specific symbols are used which show you the type of hazard Biohazard Risk of crushing Failu...

Page 8: ...ance procedures described in this manual are excepted and may be carried out by the user l The manufacturer can accept no liability for this product if the provisions of this documentation are not com...

Page 9: ...environment BSL 2 is suitable for work involving agents of moderate potential hazard to personnel and the environment All procedures performed on the Operetta should be done carefully to minimize the...

Page 10: ...that require access to the instrument while the interlocks are defeated may only be carried out by qualified service personnel Service personnel must observe the instructions of the separate Service M...

Page 11: ...w into the instrument This corresponds approximately to the amount of carbon dioxide which one exhales during one hour and can be considered as relatively safe However the flow limiter does not protec...

Page 12: ...the provided power cable l Check the power cable for damages before switching on the Operetta Do not use the instrument with damaged power cable l In case of a damaged power cable contact PerkinElmer...

Page 13: ...ing the outer surface of the Operetta use a slightly moistened damp cloth For cleaning the workstation inside the operator must specify suitable precautions especially with regard to biological contam...

Page 14: ...cause harmful interference to radio or television reception which can be determined by turning the equipment off and on the user is encouraged to try to correct the interference by one or more of the...

Page 15: ...erference with the equipment including but not limited to the removal or exchange of parts and the use of accessories of other origin Any such action or negligence will invalidate any claim under warr...

Page 16: ...1 7 Conformity 1 System Safety 16 Operetta Technical Manual...

Page 17: ...vibrations Blurred images can be the consequence l Put the Operetta on a very stable bench or table l Do not put other devices on the same table which cause vibrations e g a centrifuge l Avoid any sho...

Page 18: ......

Page 19: ...2 Hardware Description 2 Hardware Description Operetta Technical Manual 19...

Page 20: ...entials of setting up an HCS assay and how to use the Operetta and image analysis software for the most common applications 2 1 1 Instrument Features l High content imaging reader l Switchable between...

Page 21: ...ot installed and if you reach into the danger area of table or robot l Do not use an Operetta without protective shields installed if the scanning table flap is unlocked l The table in out functionali...

Page 22: ...Barcode Reader Port for connecting a PerkinElmer barcode reader Notice Please use a PerkinElmer barcode reader only otherwise a faultless operation cannot be guaranteed 2 2 3 Front Side 1 Filter acce...

Page 23: ...on prior permission from Harmony o Exchange sample carrier Use the Open Lid button in Harmony For details please see Operetta Operation Manual section 3 15 Open Lid o Exchange or clean objective Use t...

Page 24: ...nt is in operation measuring The LEDs indicate the progress of the measurement on a percentage basis Blinking Blue Red Waiting for User The instrument is waiting for user interaction This does not ind...

Page 25: ...pen the sample lid In contrast to the status light this is no status indication see also previous chapter 2 Scanning stage with plate holder o Mechanism to hold or release the sample carrier o Sample...

Page 26: ...ctives 2x 10x 20x 40x 60x 100x long WD and high NA 4 Transmission light source for brightfield excitation o Detect cells independent of dye label free techniques o 740nm Infrared to minimize cell dama...

Page 27: ...one hour to reach and stabilize the target temperature and 5 min to stabilize the selected CO2 concentration Inside the Operetta there is an air circulating unit which regulates the CO2 level in the s...

Page 28: ...in the sample chamber is lost and you have to wait until the target values have been reached and stabilized again Inside the Live Cell Chamber there are heating elements for controlling the temperatur...

Page 29: ...nger Carbon Dioxide Gas Supply Tubing l Do not expose the tubing to any mechanical or thermal stress l Comply with the maximum bending radius of the tubing see next page l If the tubing has to be laid...

Page 30: ...ng o Outer diameter 6 mm o Max bending radius 21 mm o Shore hardness D 62 3 o Material TPE A polyamide l Working pressure 3 bar l Required CO2 flow 15 l h l Required CO2 purity 99 998 particle free No...

Page 31: ...y settings on the Harmony PC Please see chapter 4 IT Policy page 44 2 5 Xenon Light Source The Operetta is equipped with an external Xenon light source Notice This chapter is an overview for the most...

Page 32: ...t Accepts ACMI Olympus Storz or Wolf fiber optic light cables The names of the ports are clearly visible on the perimeter of the adapter outboard from the port inputs Operetta ACMI Danger Danger of wh...

Page 33: ...3 Retooling and Maintenance 3 Retooling and Maintenance Operetta Technical Manual 33...

Page 34: ...closely and observe both the general safety instructions see chapter 1 System Safety page 5 and any specific safety instructions which may be given at the beginning of each section 3 1 Retooling 3 1 1...

Page 35: ...n the glass plate inside the Operetta for 30 60 minutes Follow the instruction of the Objective Exchange Wizard Harmony Settings Change Objective 1 If all four positions of the turret are occupied Sel...

Page 36: ...pe will also be stated Also please note that while the message box Please adjust the correction collar is displayed the table is positioned so as to give easy access to the objective The objectives ha...

Page 37: ...m adjustment is shown How to Adjust the Correction Collar Objective with correction collar 1 If the message Please adjust the correction collar to mm appears open the sample lid 2 Turn the correction...

Page 38: ...long WD Yes 0 0 7 3 1 3 Emission Filter The Operetta is equipped with an eight position emission filter wheel The four emission filters 410 480 nm 500 550 nm 560 630 nm and 650 760 nm are always incl...

Page 39: ...can damage the instrument 1 Open the filter access door to reach the filter wheel 2 If necessary remove the old filter 3 Insert the new filter to the intended position 4 Close the door The filter whee...

Page 40: ...symptoms indicating the need for replacement are l Repeated ignition attempts without lamp going into operation l Low light output evident with dark picture at high light level adjustment The deliver...

Page 41: ...be cleaned using 70 ethanol l The glass panels should be cleaned with a suitable glass cleaning agent l Do not handle with greater amounts of fluids near the device Cleaning the Objective Lens Caution...

Page 42: ...fective fuse may only be replaced by exactly the same part from the following manufacturer LITTLEFUSE 218000 Series 5 20 mm Time Lag Fuse Slo Blo 15 A 250 V US 8 A 250 V EU 1 Switch off the Operetta a...

Page 43: ...rried out as specified to guarantee the correct and reliable operation of the Operetta Warning The jobs listed here may only be performed by the PerkinElmer Service l If you do not have a maintenance...

Page 44: ...4 IT Policy 4 IT Policy 44 Operetta Technical Manual...

Page 45: ...ible for problems caused by violating the following policies Any effort required to verify this type of problem is billable at the current service rate and is not covered by guarantee and or service c...

Page 46: ...ion General Network Requirements l The subnet mask for all network adapters has to be 255 255 255 0 l All network connections must be Gigabit Ethernet connections using CAT 5e cable All devices shown...

Page 47: ...llowed in the customer s company network use one of the following solutions to isolate the Operetta network from the company network 4 1 3 1 Office PC as interface small solution 4 1 3 2 Office PC as...

Page 48: ...4 1 3 3 Router 4 IT Policy 48 Operetta Technical Manual...

Page 49: ...rated into a domain l Anti virus or malware protection software may not be installed on this computer 4 1 4 1 Standard Configuration This scenario is only possible if NIC2 of the Harmony PC and NIC1 o...

Page 50: ...ervice Pack 3 only for office PC installations This includes all security patches until October 1st 2010 Notice PerkinElmer cannot guarantee that future Windows updates provided by Microsoft will not...

Page 51: ...current service rate PerkinElmer may not provide maintenance service on the computers supplied by the customer Basic l Harmony Office License incl USB dongle with valid Software Maintenance Agreement...

Page 52: ...hould be discussed in advance with PerkinElmer service to assess the detailed specification Problems with hardware should also be reported to PerkinElmer service 1 The relocation of the Harmony PC is...

Page 53: ...ony software is tested with Microsoft Forefront Client Security 1 PerkinElmer is not responsible for the implementation of an effective virus security strategy It is suggested to exclude the following...

Page 54: ...manually must be accessible for the default user account Operetta read write access including the right to create subfolders 4 Passwords Operetta System users should not change any passwords Any chan...

Page 55: ...5 Technical Data 5 Technical Data Operetta Technical Manual 55...

Page 56: ...The illumination intensity in the sample can be tuned in the software using a mechanical attenuator The fluorescence excitation wavelength is selected by choosing an excitation filter on an eight pos...

Page 57: ...citation filters four dichroics four emission filters 20x objectives camera Xenon lamp laser based auto focus and one set of Harmony Software included in the instrument Please see the following table...

Page 58: ...axis Plate holder Automation plate handler II optional 5 2 1 Detection Components Detector CCD camera with 1 3 mega pixel Peltier cooled 14 bit resolution Emission band pass changer 5 2 2 Light Source...

Page 59: ...WD 0 7 1 5 2 2 225 170 1 9 0 43 60x high NA 0 9 0 2 225 170 1 1 0 33 100x long WD 0 85 1 2 1 9 135 102 1 3 0 35 5 3 2 Sample Carrier Recommended sample carriers for the Operetta Plate type Bottom mate...

Page 60: ...ured 5 3 3 Power Supply The Operetta is connected to mains by a separate power cable which is specified as follows Specifications Europe US Cordage Rating 16A 250V AC 15A 125V ACl Plug 16A 250V AC IEC...

Page 61: ......

Page 62: ...PerkinElmer Schnackenburgallee 114 22525 Hamburg Germany Internet http www perkinelmer com email info perkinelmer com...

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