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10 . Lambda 365 Users Guide

Figure 4. Transmittance and Absorbance

Double Beam UV-Visible Spectrophotometer

Lambda 365 double-beam UV-Visible spectrophotometer, the light is split into two beams

before it reaches the sample. One beam is used as the reference; the other beam passes

through the sample. The reference beam intensity is taken as 100% Transmission (or 0

Absorbance), and the measurement displayed is the ratio of the two beam intensities.

Lambda 365 double-beam UV-Visible spectrophotometer has two detectors (photodiodes),
and the sample and reference beam are measured at the same time

Summary of Contents for Lambda 365

Page 1: ...LAMBDA 365 Users Guide UV SPECTROMETERS ...

Page 2: ...ted to the implied warranties of merchantability and fitness for a particular purpose PerkinElmer shall not be liable for errors contained herein for incidental consequential damages in connection with furnishing performance or use of this material Copyright Information This document contains proprietary information that is protected by copyright All rights are reserved No part of this publication...

Page 3: ...2 Installation 34 UV Express Software Installation 35 How to Install the UV Express Software 36 Interface Setup 41 Setting USB Serial Ports 47 Procedure 48 Operating Procedure 53 Procedure 54 Accessories 55 Accessories for the Lambda 365 56 Single Cell Holder 56 Water Jacketed Single Cell Holder 57 8 Position Multicell Holder Water Jacketd 8 Position Multicell Holder 57 Micro Cell Holder 57 Variab...

Page 4: ...4 Lambda 365 Users Guide ...

Page 5: ...Introduction 5 Introduction ...

Page 6: ...uctions for installing and operating the Lambda 365 Double Beam UV Visible Spectrophotometer and UV Express software For more detailed information on software features including Quantification kinetics and bio software refer to the UV Express software user guide ...

Page 7: ...tallation Information on installing and re installing your instrument should you ever need to move your system is provided Chapter 4 UV Express Software Installation The complete UV Express software installation procedures for the Lambda 365 is in this chapter Chapter 5 Interface Setup Interface procedures for the Lambda 365 Chapter 6 Operating Procedure This chapter contains information on operat...

Page 8: ...orption rate varies depending on the wavelength of the radiation Data from absorption spectra can be used for qualitative and quantitative analysis A molecule remains in its ground state when it is stable but it can transition to an excited state when light energy is absorbed This is termed absorption Figure 2 When the excited molecule returns to the ground state it emits heat radiation fluorescen...

Page 9: ...igure 3 Sample Absorption As a result of absorption by a sample transmittance is defined as T P P0 And Absorbance is defined as A log T log P0 P Absorbance is related to c concentration and b pathlength of light according to the Lambert Beer law and the equation A εbc Where ε the molar absorption coefficient of a particular molecule varies with the wavelength Although Beer s law has several limita...

Page 10: ...efore it reaches the sample One beam is used as the reference the other beam passes through the sample The reference beam intensity is taken as 100 Transmission or 0 Absorbance and the measurement displayed is the ratio of the two beam intensities Lambda 365 double beam UV Visible spectrophotometer has two detectors photodiodes and the sample and reference beam are measured at the same time ...

Page 11: ...ations de danger Warning When supplying power to this equipment connect the accessory 3 pin power cord to a 3 pin grounded power outlet If a grounded 3 pin outlet is not available use a conversion adapter and ground the green wire If power is supplied without grounding the equipment there is a risk of receiving a fatal electric shock and equipment damage Lors de l alimentation de l équipement bran...

Page 12: ...mage to precision parts L utilisateur ne doit pas essayer de réparer cet équipement NE PAS tenter d ouvrir le boîtier ou de démonter ses pièces internes Seul un représentant du service PerkinElmer qui est conscient des risques d incendie et d électrocution devrait réparer cet équipement Cet équipement contient des parties à haute tension présentant des risques de blessure grave ou d électrocution ...

Page 13: ...structed If the ventilation is obstructed the system may overheat and catch fire Periodically clean your equipment s case using a damp cloth Do not use abrasives cleaning solvents or strong detergents as they may damage the finish or affect the reliability of the structural components Gardez l équipement exempt de poussières Nettoyez le cordon d alimentation régulièrement Les poussières qui s accu...

Page 14: ...bda 365 Spectrophotometer shipping kit Item Name Qty Main Instrument Lambda 365 Double Beam UV Vis Spectrophotometer 1 Accessories Power Cord US EU UK 3 Interface Cable USB 1 Lambda 365 Document Pack CD 1 UV Express Software CD 1 Single Cell Holder 1 Lamp Change Tool 1 Fuse AC 250 V T3 15AL 2 Wrench M3 1 ...

Page 15: ...er part numbers unless stated otherwise Notes cautions and warnings Three terms in the following standard formats are also used to highlight special circumstances and warnings NOTE A note indicates additional significant information that is provided with some procedures Note attention et avertissement Trois termes dans les formats standard suivants sont également utilisés pour mettre des circonsta...

Page 16: ...n recuadros éste se proporciona información sobre este tipo de circunstancias F Caution Attention Nous utilisons le terme CAUTION ATTENTION pour signaler les situations susceptibles de provoquer de graves détériorations de l instrument ou d autre matériel Les détails sur ces circonstances figurent dans un encadré semblable à celui ci I Caution Attenzione Con il termine CAUTION ATTENZIONE vengono s...

Page 17: ...ecuadros como éste se proporciona información sobre este tipo de circunstancias F Warning Danger Nous utilisons la formule WARNING DANGER pour avertir des situations pouvant occasionner des dommages corporels à l utilisateur ou à d autres personnes Les détails sur ces circonstances sont données dans un encadré semblable à celui ci I Warning Pericolo Con il termine WARNING PERICOLO vengono segnalat...

Page 18: ...lmer com PerkinElmer s catalog service offers a full selection of high quality supplies To place an order for supplies and many replacement parts request a free catalog or ask for information If you are located within the U S call toll free 1 800 762 4002 8 a m to 8 p m EST Your order will be shipped promptly usually within 24 hours If you are located outside of the U S call your local PerkinElmer...

Page 19: ...Safety Practices 19 Safety Practices ...

Page 20: ... information provided does not cover every safety procedure that should be practiced Ultimately maintenance of a safe laboratory environment is the responsibility of the analyst and the analyst s organization Please consult all manuals supplied with the Lambda 365 and accessories before you start working with the instrument Carefully read the safety information in this chapter and in the other man...

Page 21: ...ent les précautions énumérées ci dessous Il est conseillé d afficher une copie des précautions sur l étagère d instrument ou à proximité The following precautions must be observed when using the Lambda 365 Be sure that the voltage of the Lambda 365 corresponds to the voltage used in your laboratory Les précautions suivantes doivent être respectées lors de l utilisation du Lambda 365 Assurez vous q...

Page 22: ... condensing Indoor only Altitude up to 2000 m If possible avoid any adjustment maintenance and repair of the opened operating instrument If any adjustment maintenance and repair of the opened instrument is necessary this must be done by a skilled person who is aware of the hazard involved Whenever it is likely that the Lambda 365 is unsafe make it inoperative The Lambda 365 may be unsafe if it sho...

Page 23: ...susceptible d exposer des parties sous tension Même quand l interrupteur d alimentation est en position Off des tensions élevées peuvent encore être présentes Des condensateurs à l intérieur de l instrument peuvent être encore chargés même si l instrument a été débranché de toutes les sources de tension The instrument must be correctly connected to a suitable electrical supply The supply must have...

Page 24: ...stment maintenance or repair this must only be done by a PerkinElmer Service engineer Whenever it is possible that the instrument is no longer electrically safe for use make the instrument inoperative and secure it against any unauthorized or unintentional operation The electrical safety of the instrument is likely to be impaired if for example the instrument shows visible damage has been subjecte...

Page 25: ...erence to radio communications However there is no guarantee that interference will not occur in a particular installation If this equipment does cause harmful interference to radio or television reception which can be determined by turning the equipment off and on the user is encouraged to try to correct the interference by one or more of the following measures Reorient or relocate the receiving ...

Page 26: ...of the instrument Warning FUSE Avertissement FUSE Warning For continued protection against electrical shock replace with same type and ratings of fuse Avertissement Pour assurer la protection contre le risque de choc électrique remplacez le fusible seulement avec le même type et la même valeur nominale Warning To prevent electrical shock do not open cover Refer all servicing to qualified personnel...

Page 27: ...he customer s environment Requirements for waste collection reuse recycling and recovery programs vary by regulatory authority at your location Contact your local responsible body e g your laboratory manager or authorized representative for information regarding applicable disposal regulations Contact PerkinElmer at the web site listed below for information specific to PerkinElmer products Web add...

Page 28: ...ontamination These documents are available on the PerkinElmer public website Procedure http las perkinelmer com Content technicalinfo dts_instrumentdeconprocedure pdf Certificate form http las perkinelmer com Content technicalinfo dts_perkinelmercertificationofdecontaminati onform pdf If you do not have access to the internet and are located in the U S call toll free at 1 800 762 4000 or 1 203 925...

Page 29: ...acés tandis que le cordon d alimentation est encore branché The fuse is located at the rear of the instrument 1 Turn off and unplug the instrument 2 Carefully open the compartment latch where the fuse is located 3 Disconnect the fuse 4 Replace with two new T3 15L fuses one for each phase 5 Close the compartment door 6 Plug in the instrument and turn on Location of latch on the fuse compartment doo...

Page 30: ...30 Lambda 365 Users Guide ...

Page 31: ...Lambda 365 Installation 31 Lambda 365 Installation ...

Page 32: ...g an electromagnetic field and any environment that may cause vibration Compte tenu des composants électroniques dans le Système Lambda 365 il doit être placé très loin de tous les autres instruments émettant des champs électromagnétiques et toutes les autres sources produisant des vibrations Power A grounded AC power supply must be used The Lambda 365 accepts 100 to 240 VAC at 50 Hz or 60 Hz WARN...

Page 33: ...0 240V 50 60Hz 110W Operating Temperature 15 35 59 95 F Operating humidity Less than 80 relative non condensing Mains Supply Voltage Functions 10 Computer Requirements Requirement Description Hardware Processor Intel Core 1 5 GHz or faster RAM At least 1GB Hard disk 50GB with 1GB free Input devices Mouse and keyboard Monitor 1024x768 minimum Media CD ROM Drive Port USB port for the data acquisitio...

Page 34: ... the power plug must be grounded WARNING The electric power could be dangerous Before connecting the power cord verify that the main power switch is off Le courant électrique peut être dangereux Avant de brancher le cordon d alimentation vérifiez que l interrupteur du secteur soit en position Off WARNING Unpacking Heavy Weight The Lambda 365 weighs 23 5 kg To avoid injury to personnel or damage to...

Page 35: ...UV Express Software Installation 35 UV Express Software Installation ...

Page 36: ...wer Options from the Start menu in the Windows operating system b Click Power saver and click Change plan settings c Click the Put the computer to sleep drop down list and then click Never in the Edit Plan Settings window Click Save Changes 1 Insert the UV Express installation CD into the CD ROM drive InstallShield Wizard starts automatically 2 InstallShield Wizard does not start automatically sel...

Page 37: ...Interface Setup 37 3 UV Express InstallShield Wizard starts Select Next ...

Page 38: ...ide 4 Enter customer information if necessary and select Next 5 The software will be installed in C UV Express as a destination folder To install the software to a different folder select Change and select the desired folder ...

Page 39: ...Interface Setup 39 6 Verify the current settings and select Install 7 The following window shows the status of the UV Express installation ...

Page 40: ...40 Lambda 365 Users Guide 8 After the setup is completed successfully select Finish The UV Express software icon will appear on the Desktop ...

Page 41: ...Interface Setup 41 Interface Setup ...

Page 42: ...ambda 365 Users Guide USB Interface Setup 1 Connect the USB cable between the computer and the instrument 2 Turn on the computer and the instrument 3 Click Computer CD Drive Open 4 Select USB Driver folder ...

Page 43: ...Interface Setup 43 5 Double click USBCom CDM_20828 6 Select Extract ...

Page 44: ...44 Lambda 365 Users Guide 7 Select Next 8 The following dialog box will appear After installation is completed successfully select Finish ...

Page 45: ...Interface Setup 45 9 Change the USB serial Ports after finishing driver setup See the following chapter for that procedure ...

Page 46: ...46 Lambda 365 Users Guide ...

Page 47: ...Setting USB Serial Ports 47 Setting USB Serial Ports ...

Page 48: ...48 Lambda 365 Users Guide Procedure 1 Select Computer Properties 2 Click on Device Manager ...

Page 49: ...orts COM LPT to expand the listing These are the devices currently connected to the COM ports USB Serial Port COMx is visible when the driver installation is completed successfully 4 Double click USB Serial Port COMx of the Ports COM LPT section ...

Page 50: ...50 Lambda 365 Users Guide 5 Select on the Port Settings tab and click on the Advanced button 6 Change the parameter values as shown below ...

Page 51: ...9 If the Lambda 365 fails to communicate with the PC change the COM Port Number as following procedure 10 Open Advanced Setting for COMx window following on page 48 50 11 Select the COM Port number list to expand it and change the COM port number another one which is not in use from COM 1 to COM 10 ...

Page 52: ...52 Lambda 365 Users Guide 12 Make sure that the changed COM Port Number is applied and select OK 13 Restart the computer after finishing driver setting ...

Page 53: ...Operating Procedure 53 Operating Procedure ...

Page 54: ...marrez pas le logiciel UV Express avant d avoir terminé l initialisation du réseau 2 Double click UV Express folder 3 Execute Scan mode icon in the UV Express Software folder The following message box will be displayed Empty the cell holder and close the lid firmly Click OK 4 Start System Self Test System Self Test is the procedure that the system checks instrumental conditions by itself System Se...

Page 55: ...Accessories 55 Accessories ...

Page 56: ...from PerkinElmer using the part numbers quoted in the guides provided with the instrument See our website http perkinelmer com PerkinElmer s catalog service offers a full selection of high quality supplies To place an order for supplies and many replacement parts request a free catalog or ask for information If you are located within the U S call toll free 1 800 762 4002 8 a m to 8 p m EST Your or...

Page 57: ...ed circulator Optional stirring is available with a separately purchased stir controller 8 Position Multicell Holder Water Jacketd 8 Position Multicell Holder Use this accessory to measure eight samples automatically Automatic Water Jacketed 8 Position Multicell Holder accommodates a temperature by circulator These accessories are controlled by UV Express software Micro Cell Holder Use this access...

Page 58: ...m pathlengths A moveable block secures cells in position Advanced Transmission Holder Use this accessory to measure the transmittance spectrum of various solid samples including films glass filters and optical components Film Holder Use this accessory to measure the transmittance spectrum of thin samples such as films and filters ...

Page 59: ...ding to the variable angle 50 mm Transmission Reflectance Sphere Used for measuring the reflectance or total transmittance of solid samples such as metals glass textiles paper gems and powders Fixed Angle Reflectance Holder Use this accessory to test coatings and semi conductor wafers especially effective for measuring specular reflectance ...

Page 60: ...instrument This accessory is controlled by UV Express software with heated reference without heated reference Peltier Controller 6 Position Peltier Controlled Cell Changer Controller Control the temperature of up to six 10 mm pathlength rectangular cell between 5 and 100 Peltier control offers fast ramping with recirculating liquid for exceptional temperature control Integrated purging capability ...

Page 61: ...essory to measure micro volume sample such as DNA RNA and Protein Autosampler Automate the measurement of up to 360 samples at a time with an autosampler accessory Two and four rack configurations are available This accessory system including the automated sipper is fully controlled by UV Express software ...

Page 62: ...62 Lambda 365 Users Guide ...

Page 63: ...Accessories 63 Lamp Replacement ...

Page 64: ... bulb section of the lamp this can leave oil deposits on the bulb which can drastically reduce lamp life Utiliser des gants jetables appropriés pendant le processus de remplacement de la lampe Evitez également de toucher la section de l ampoule de verre de la lampe cela peut laisser des dépôts d huile sur le bulbe qui peut réduire considérablement la vie de la lampe 2 Remove the white bolt covers ...

Page 65: ...ips screwdriver Note that there is no need to take the screws out from the lamp module 5 Carefully lift up the D2 lamp module and disconnect the D2 lamp cable connector 6 Replace the D2 lamp module with a new one and reassemble the parts in reverse order ...

Page 66: ...id touching the glass bulb section of the lamp this can leave oil deposits on the bulb which can drastically reduce lamp life Utilisez Tungsten Lamp outil Modification lors du remplacement de la lampe de tungstène En outre une paire de gants jetables appropriés doivent être portés pendant le processus de remplacement de la lampe S il vous plaît éviter de toucher la section de l ampoule de verre de...

Page 67: ...67 3 Remove the two M3 6 screws on the sub cover using a Phillips screwdriver 4 Put the tungsten lamp changing tool on the tungsten lamp module carefully by aligning the notches over the lamp module securing screws ...

Page 68: ... Users Guide 5 Fasten the cap screw on the tungsten lamp changing tool to fix the tool to the tungsten lamp module 6 Using a Phillips screwdriver to loosen the two screws that are located on the tungsten lamp module ...

Page 69: ... the tungsten lamp module and disconnect the tungsten lamp cable connector 8 Loosen the cap screw on the tungsten lamp changing tool Part No N4102048 and separate the tungsten lamp from the tool 9 Replace the tungsten lamp with a new one and reassemble the parts in reverse order ...

Page 70: ... 15 text 15 D Decontamination 28 E Electrical Safety 23 EMC Compliance 25 Environmental Requirements 33 I Installation Lambda 365 34 UV Express Software 36 L Laboratory Preparation 32 Lamp Replacement D2 Lamp 64 Tunsten Lamp 66 O Operating Conditions 22 Operating Procedure 48 P Precautions 21 S Safety Summary 11 Safety Information 21 electrical safety 23 Shipping Kit 14 U UV Express Software Insta...

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