Using the Colorimeter
Using the Colorimeter
Use the colorimeter to measure the absorbance and transmittance of
six different wavelengths.
Step 1: Pr
Step 1: Prepar
epare Solutions for Analysis
e Solutions for Analysis
When preparing solutions for analysis:
• Use the lined cuvettes.
• Handle the cuvettes by the lined sides to avoid getting fingerprints
on the smooth sides.
• Fill cuvettes ¾ full with the test solution and cap the cuvette.
• Clean the smooth sides of the cuvette using a lint-free wipe before
inserting it into the test chamber.
Step 2: Pr
Step 2: Prepar
epare a Blank Calibr
e a Blank Calibration Cuv
ation Cuvette
ette
Fill one lined cuvette ¾ full with the solvent used to prepare the test
solutions. The solvent is used to calibrate the colorimeter.
Step 3: Set Up Data Collection
Step 3: Set Up Data Collection
SP
SPARK
ARKvue
vue
1. Turn on the Wireless Colorimeter and Turbidity sensor.
2. Open SPARKvue then click Sensor Data
Sensor Data
.
3. Select the Colorimeter that matches the device ID.
4. Select a Quick Start Experiment.
◦
Absorbance and Transmittance
Absorbance and Transmittance
Measure absorbance and transmittance of a solution at six
different wavelengths on a bar meter display.
◦
Beer's Law
Beer's Law
Plot absorbance versus concentration of known solutions to
determine the unknown concentration of a solution.
P
PASCO Capst
ASCO Capstone
one
1. Turn on the Wireless Colorimeter and Turbidity sensor.
2. Open Capstone then click Hardware Setup
Hardware Setup
.
3. Select the Colorimeter that matches the device ID.
Note:
Note: You may be prompted to calibrate the sensor. If you
want to calibrate now, click Calibrate
Calibrate and proceed to the
Calibrate the Sensor
Calibrate the Sensor section. Otherwise, click Cancel
Cancel.
4. Click Hardware Setup
Hardware Setup
to close the panel.
5. Select a Quick Start Experiment.
◦
Absorbance and Transmittance
Absorbance and Transmittance
Measure absorbance and transmittance of a solution at six
different wavelengths on a bar meter display.
◦
Beer's Law
Beer's Law
Plot absorbance versus concentration of known solutions to
determine the unknown concentration of a solution.
Step 4: Calibr
Step 4: Calibrate the Sensor
ate the Sensor
SP
SPARK
ARKvue
vue
1. Click Hardware Setup
Hardware Setup
then click
for the Wireless
Wireless
Colorimeter
Colorimeter.
Note:
Note: You may be prompted to calibrate the sensor. Click
Calibrate
Calibrate, if prompted.
2. Insert the blank calibration cuvette into the Colorimeter with the
smooth side facing the sensor and close the cover.
3. Click Calibrate
Calibrate. A green check
will indicate that the colorimeter
is calibrated.
4. Click Done
Done.
5. Close Hardware Setup.
P
PASCO Capst
ASCO Capstone
one
1. Click Calibration
Calibration
.
2. Select Wireless Colorimeter
Wireless Colorimeter from the list then click Next
Next.
3. Insert the blank calibration cuvette into the Colorimeter with the
smooth side facing the sensor and close the cover.
4. Click Calibrate
Calibrate. A green check
will indicate that the colorimeter
is calibrated.
5. Click Finish
Finish.
6. Click Calibration
Calibration
to close the panel.
Step 5: Collect Data (Absorbance and T
Step 5: Collect Data (Absorbance and Trransmittance)
ansmittance)
SP
SPARK
ARKvue
vue
1. Insert a cuvette containing your test sample with the smooth side
facing the sensor and close the cover.
2. Click Start
Start
to begin data collection.
3. Click Stop
Stop
to end data collection.
4. Repeat the steps for additional test samples.
P
PASCO Capst
ASCO Capstone
one
1. Insert a cuvette containing your test sample with the smooth side
facing the sensor and close the cover.
2. Click Record
Record
to begin data collection.
3. Click Stop
Stop
to end data collection.
4. Repeat the steps for additional test samples.
Step 5: Collect Data (Beer
Step 5: Collect Data (Beer''s Law)
s Law)
SP
SPARK
ARKvue
vue
1. Click Start
Start
to begin data collection.
2. Insert a cuvette containing a solution of known concentration
with the smooth side facing the sensor and close the cover.
3. Enter the known concentration in the table.
4. Click Keep
Keep
to record the Absorbance measurement.
5. Repeat steps 2-4 for each known solution.
6. Click Stop
Stop
to end data collection.
P
PASCO Capst
ASCO Capstone
one
1. Click Preview
Preview
to begin data collection.
2. Insert a cuvette containing a solution of known concentration
with the smooth side facing the sensor and close the cover.
3. Enter the known concentration in the table.
4. Click Keep Sample
Keep Sample
to record the Absorbance measurement.
5. Repeat steps 2-4 for each known solution.
6. Click Stop
Stop
to end data collection.
Wireless Colorimeter and Turbidity Sensor | PS-3215
Product Guide
3
012-15830B