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HOBO Dissolved Oxygen Logger (U26-001) Manual 

                            

 5  

 

Do not deploy the logger in freezing water with moving ice 
where the logger could be crushed. 

 

Use the optional anti-fouling guard to protect against 
fouling. Unscrew the protective guard and replace it with 
the anti-fouling guard. 

 

If fouling is expected during deployment, use field 
calibration readings from both the beginning and end of 
the deployment as described in the next section. These 
readings can then be entered into the HOBOware 
Dissolved Oxygen Assistant to compensate for any 
measurement drift due to fouling. Scrub fouling off the 
logger with a plastic bristle brush. 

 

When deploying the logger in saltwater with small 
changes in salinity, you will need a conductivity or salinity 
value from either a conductivity meter or salinometer to 
enter in the Dissolved Oxygen Assistant to adjust the data 
from the logger for salinity. A single meter reading will 
add less than 1.1% DO error (assuming the conductivity 
changes are within ±3,000 µS/cm from the calibration 
point).  

If the conductivity changes, then you will need a data file 
with salinity or specific conductivity readings for the 
entire deployment. Consider deploying a HOBO 
Conductivity logger (U24-002-C) next to this DO logger to 
use the resulting data file for salinity data. For U24-002-C 
conductivity readings within a ±30,000 µS/cm range, 
there will be less than 4% error added to the DO 
measurements, and for readings over a narrower range, 
the accuracy will be even better. Refer to the 

HOBO 

Conductivity Logger (U24-002-C) Manual

 for more 

details. For applications that require higher accuracy 
conductivity data than the U24-002-C can provide, use a 
third-party conductivity logger. 

 

To generate a percent saturation series, you will need to 
deploy a barometric pressure logger (such as a HOBO 
Water Level Logger, U20-001-0x or U20L-0x) or have 
access to a nearby weather station to gather barometric 
pressure data. This data is necessary for the Dissolved 
Oxygen Assistant to calculate percent saturation. 

Taking Field Calibration Readings 

If fouling is expected during the deployment, you can take 
calibration readings at the beginning and end of the 
deployment to enter in the Dissolved Oxygen Assistant. This will 
adjust the data from the logger to compensate for any 
measurement drift due to fouling. There are two methods for 
taking field calibration readings: the first method involves 
taking readings using a dissolved oxygen meter or titration 
while the second method involves calibrating the logger in 
100% water-saturated air. The first method is recommended 
because it is quicker to get the necessary calibration readings; 
the second method can take 40 minutes or more to achieve 
equilibrium with temperature extremes.  

To Take Calibration Readings Using a DO Meter or Titration: 

1.

 

The logger must be logging. Take a DO measurement of the 
water where the logger is being deployed using either a DO 
meter or by titration. If using a meter, make sure it is 

calibrated and allow time for the meter probe to stabilize 
(this will occur when three meter measurements taken in a 
row are within your accuracy tolerance). 

If the logger is being deployed in saltwater, adjust the 
meter measurements for salinity using a meter with both 
conductivity and DO probes. If the saltwater has a constant 
salinity, you can use a DO meter where you can enter that 
salinity value to adjust the readings. If the salinity and/or 
DO are changing rapidly, then you will need to get a sample 
of the water in a container large enough for both the logger 
and meter probe to be completely submerged. Place both 
devices in the water long enough for them to stabilize and 
then for the DO logger to log at least two values, and take a 
concurrent meter reading. 

2.

 

Record the reading, date, and time of the measurement in a 
field notebook. 

3.

 

At the end of the deployment, repeat steps 1 and 2. 

To Take Calibration Readings Using 100% Water-Saturated Air: 

1.

 

The logger must be logging. You will need fresh water, the 
included calibration boot and sponge, and the current 
barometric pressure from a HOBO U20 or U20L Water Level 
logger, a barometer, or a nearby weather station. 

2.

 

If the logger has been in salt water, clean the logger body 
and sensor cap as described in the 

Maintenance

 section. 

Make sure the sensor cap is dry before continuing. 

3.

 

Make sure the protective guard or anti-fouling guard is 
installed on the logger. 

4.

 

Wet the small sponge with fresh water. Squeeze out any 
excess water. 

5.

 

Place the sponge in the end of the calibration boot. 

6.

 

Insert the logger in the calibration boot so that there is 
approximately a 1 cm (0.5 inch) overlap between the end of 
the boot and the body of the logger. This will ensure there 
is enough space between the end of the logger and the 
sponge (the logger should not be pressed up tightly against 
the sponge). 

7.

 

Allow at least 40 minutes for the logger to reach 
temperature equilibrium, and then write down the date and 
time in a field notebook. 

8.

 

Write down the barometric pressure at that time (note the 
elevation if the barometric reading has been adjusted for 
sea level). 

9.

 

Repeat these steps at the end of the deployment. 

Reading Out the Logger and Redeploying 

Your readout and maintenance schedule will be determined by 
the amount of fouling at the site. To read out the logger in the 
field: 

1.

 

Take a field calibration reading as described in the 

Taking 

Field Calibration Readings

 section. 

2.

 

If the logger was in saltwater and you did not deploy a 
HOBO Conductivity Logger, then use a conductivity meter 
or salinometer to take a conductivity reading. Write down 
the reading and the date and time. 

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