16
31. Place the plate on a magnetic separation device to magnetize the Mag-Bind®
Particles CNR. Let sit at room temperature until the Mag-Bind® Particles CNR are
completely cleared from solution.
32. Transfer the cleared supernatant containing purified RNA into a new RNase-free
microplate.
33. Store eluted RNA at -70°C.
Note:
Any combination of the following steps can be used to help increase RNA yield.
•
Heat the DEPC Water to 70°C before adding to the beads.
•
Increase the incubation time to 5 minutes.
•
Increase the elution volume.
•
Repeat the elution step with fresh DEPC Water (this may increase the yield, but
decrease the concentration).
M2839 Mag-Bind® Blood RNA 96 Protocol
