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Mag-Bind® Blood RNA 96 Kit Protocol (M2837) - 50 µL blood
The following protocol is designed for isolating total RNA from 50 μL fresh whole blood.
For best RNA quality, always use blood that has not been frozen. Frozen blood can be used
with this protocol, however, RNA quality could be compromised as the result of freeze-
thaw process.
Materials and Equipment to be Supplied by User:
•
Magnetic separation device for 96-well microplates (Recommend Cat#MSD-01)
•
Nuclease-free 500 µL 96-well microplates (Recommend Cat# EZ9604-01)
•
Multichannel
pipette
•
Nuclease-free pipette tips
•
100% ethanol
•
Isopropanol
•
Sealing film
Before Starting:
•
Prepare RNA Wash Buffer II and RXT Wash Buffer according to the “Preparing
Reagents” section on Page 6.
1. Add 65 μL RBL Buffer and 65 µL isopropanol to each well of a 500 µL 96-well
microplate.
2. Add 50 μL blood sample to each well and shake for 1 minute.
3. Add 5 μL Proteinase K Solution and 5 µL Mag-Bind® Particles CNR to each well. Pipet
up and down 10 times and shake for 5 minutes to mix thoroughly.
Note:
Proteinase K Solution must be added after the blood sample has been added
to RBL Buffer. Mag-Bind® Particles CNR and Proteinase K Solution can be made as a
mastermix.
4. Let sit at room temperature for 10 minutes.
5. Place the plate on a magnetic separation device to magnetize the Mag-Bind®
Particles CNR. Let sit at room temperature until the Mag-Bind® Particles CNR are
completely cleared from solution.
M2837 Mag-Bind® Blood RNA 96 Protocol
