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Introduction and Overview
The Mag-Bind® Blood RNA 96 Kit is designed for rapid and reliable isolation of total
and viral RNA from mammalian whole blood. The Mag-Bind Bead technology provides
high-quality RNA, which is suitable for direct use in most downstream applications, such
as amplifications and enzymatic reactions. These protocols can be easily adapted to an
automated system and the procedure can be scaled up or down.
If using the Mag-Bind® Blood RNA 96 Kit for the first time, please read this booklet to
become familiar with the procedure and its various modifications. Samples are lysed in
a specially formulated buffer containing detergent and chaotropic salt. After adjusting
the buffer conditions, nucleic acids (DNA/RNA) will form a complex with magnetic beads.
The beads/nucleic acids complex is separated from lysates using a magnet. Proteins and
cellular debris are efficiently washed away by a washing step. Next, DNA is removed with
a Mag-Bind® DNase I treatment. RNA is rebound and cleaned from the Mag-Bind® DNase
I reaction mixture using a second magnetic bead binding and washing procedure. Pure
RNA is eluted in nuclease-free water or low ionic strength buffer. Purified RNA can be
directly used in downstream applications without the need for further purification.
New in this Edition:
This manual has been edited for content and redesigned to enhance
user readability.
•
Proteinase K Solution can also be stored at room temperature for 6 months. For
storage >6 months, store at 2-8°C.
•
DNase I has been replaced by Mag-Bind® DNase I. This is a name change only.
•
Proteinase K is now supplied in a liquid form eliminating the resuspension step prior
to use.
•
Proteinase K Solution can also be stored at room temperature for 12 months.
•
Proteinase Storage Buffer is no longer included in the kit.
