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Please use this guide to troubleshoot any problems that may arise. For further assistance,
please contact the technical support staff, toll free,
at
1-800-832-8896
.
Problem
Cause
Solution
Low RNA Yield
Incomplete resuspension
of magnetic particles
Resuspend the magnetic particles by
vortexing before use
RNA degraded during
sample storage
Make sure samples are stored properly
and that the samples are processed
immediately after collection or removal
from storage
RNA Wash Buffer II was
not prepared correctly
Prepare RNA Wash Buffer II by adding
ethanol according to the instructions
Loss of magnetic beads
during procedure
Increase the bead collection time
Blood clots cause
congregation of magnetic
beads
Make sure the sample is clear of blood
clots before adding magnetic beads.
Problem
Cause
Solution
No RNA eluted
RNA Wash Buffer II was
not diluted with 100%
ethanol
Prepare RNA Wash Buffer II by adding
ethanol according to the instructions
Problem
Cause
Solution
Problem with
downstream
applications
Insufficient RNA was used
RNA is already degraded; always use fresh
blood for RNA isolation
Quantify the purified RNA accurately and
use sufficient RNA
Problem
Cause
Solution
Carryover
of magnetic
beads during
elution
Carryover of magnetic
beads in the eluted
RNA will not effect
downstream applications
To remove the carryover magnetic beads
from eluted RNA, simply magnetize the
magnetic beads and carefully transfer to
a new plate
Problem
Cause
Solution
DNA
contamination
Inefficient DNase I
digestion
Make sure to use proper starting material
Ensure that the DNase I digestion is
carried out at room temperature
Troubleshooting Guide
