Olympus BX51WI Instructions Manual Download

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Summary of Contents for BX51WI

Page 1: ...WI To ensure the safety obtain optimum performance and to familiarize yourself fully with the use of this microscope we recommend that you study this manual thoroughly before operating the microscope...

Page 2: ......

Page 3: ...ing the Fine Adjustment Fast Feed Knob 3 Using the Frost Switching Lever 4 Adjusting the Coarse Adjustment Knob Rotation Tension 1 2 Correct assembly and adjustments are critical for the microscope to...

Page 4: ...OF THE POWER SUPPLY CORD 43 44 19 30 NOTE This equipment has been tested and found to comply with the limits for a Class A digital device pursuant to Part 15 of the FCC Rules These limits are designe...

Page 5: ...ymbols The following symbols are found on the microscope Study the meaning of the symbols and always use the equipment in the safest possible manner Symbol Explanation Indicates that the surface becom...

Page 6: ...set off text in this instruction manual Indicates that failure to follow the instructions in the warning could result in bodily harm to the user and or damage to equipment including objects in the vi...

Page 7: ...TR30IR FN 22 U ETR3 FN 22 Revolving Arm WI NPA Revolving Nosepiece WI SRE3 WI SNPXLU2 U SLRE Cross Stage IX SVL2 or commercially available bridge stage Fixed Stage Adapter WI FSH Condenser WI UCD WI...

Page 8: ...ge center plate Y axis knob Page 12 X axis knob Page 12 Rubber cap for fine adjustment knob Page 11 Pre focusing lever Page 11 Coarse adjustment knob Page 11 Light path selector knob Page 15 Reflected...

Page 9: ...MSAD Indication label attaching frame Filter positions Accepts 32 mm filters with thickness of no more than 6 mm Polarizer clamping knob Page 19 Polarizer rotating dials Page 23 Polarizer positions Pa...

Page 10: ...e 20 UIS2 UIS objective mount screws Turret 4 positions Quarter wave plate clamping knob Quarter wave plate rotation ring Page 23 Optical element index mount Page 21 DIC prism replacement cover Quarte...

Page 11: ...Aperture iris diaphragm lever Page 17 High Resolution DIC Prism A WI DICTHRA2 DIC Prism WI DICT2 This prism can be mounted in the DIC prism position of the WI SNPXLU2 or WI SRE3 Applicable condensers...

Page 12: ...a low magnification objective in the light path Swing lever P 13 Bring the specimen in focus Coarse fine adjustment knobs P 11 Adjust the brightness Light intensity control knob P 10 Adjust the inter...

Page 13: ...9 BX51WI Make a photocopy of the observation procedure pages and post it near your microscope Used only when the hand switch is not connected...

Page 14: ...with the intensity control knob 2 To turn the lamp OFF press the lap ON OFF switch again to OFF The lighting of the REMOTE LED indicates that the hand switch is standing by The hand switch consumes a...

Page 15: ...ng the Fine Adjustment Fast Feed Knob Fig 6 Fine focusing is usually possible while the rubber cap is attached However when it is desirable to allow the fine adjustment knob to vary focusing by a larg...

Page 16: ...Fig 10 The screw hole may sometimes be stuck by paint etc In such a case screw in the M4 screw a few times to expose the metallic thread inside the screw hole and improve the contact before attaching...

Page 17: ...er holder See page 42 for details 4 4 Revolving Nosepiece If the petri dish in use is filled with liquid it may splash when the objective is switched As such liquids are sometimes toxic be sure to mov...

Page 18: ...focus on the specimen Fig 16 Using a Finder Eyepiece 1 Looking through the right eyepiece with your right eye turn the top of the eyepiece until a clearly defined double crosslines can be seen in the...

Page 19: ...r eyeglasses Use 24 mm dia x 1 5 mm micrometer disks Following Fig 19 remove the micrometer mounting frame from the eyepiece and place a micrometer disk into the mounting frame Re attach the micromete...

Page 20: ...ing screws on the condenser holder to move the iris diaphragm image to the center of the field of view Fig 21 Fig A Fig B 7 Gradually open the field iris diaphragm The condenser is properly cen tered...

Page 21: ...ique illumination This is possible with objectives from 5X to 100X When the XLUMPlanFI20XW objective is used the effects of oblique illumination cannot be manifested fully due to the high NA 0 95 of t...

Page 22: ...me refractive index water The electrically insulated area and immersion depth of the objective are shown by the range of Do not immerse the entire objective for this will cause malfunction After every...

Page 23: ...taching the Polarizer Fig 29 The performance of polarizer drops after it has been subjected to light for long hours Replace it after about 500 hours of continuous use Drop the polarizer into the filte...

Page 24: ...observation Surface layer only 4 Attaching the DIC Prisms for Condenser Figs 32 35 DIC prisms can be inserted in three types of condensers including the WI UCD WI DICD and U UCD8 Do not use the U UCD...

Page 25: ...in the DIC prism by aligning its positioning pin with the positioning groove in the hole of the turret Fig 33 Be careful not to touch the prism inside the frame A Rotate the turret by 90 clockwise Fi...

Page 26: ...len screwdriver provided with the microscope then place the top of the condenser upside down 3 When the DIC prism for use with the objective in use is a small DIC prism drop it in by aligning the posi...

Page 27: ...early visible when the specimen is thick In this case it is recommended to bring a scratch or like on the bottom of the petri dish to facilitate the subsequent adjustment operation With the WI DICD do...

Page 28: ...IR observation is suitable for observing deep areas of a thick brain slice or optic nerve specimen 1 Introduction 1 Since the IR wavelength used is 775 to 900 nm the TV camera in use should be sensiti...

Page 29: ...to c below With IR observation the combination with the U PMTVC or U DPT cannot manifest full performance a Combination for observing a wide field direct image 1X Direct image adapter U TV1X Mount ada...

Page 30: ...ction of the lamp housing insert the filter in the lamp housing and clamp by tightening the ring spring provided with the filter 3 Attach the collector lens to the original position IR Filter 32BP775...

Page 31: ...open in this adjustment so that the optical performance can be manifested fully 2 Then engage the IR filter 32BP775 to 32IR900 in the light path by pushing in the filter slider 3 While observing the...

Page 32: ...U UCD8 swinging condenser If other condenser is combined it will not be possible to illuminate the entire field of view 3 During low magnification fluorescence observation objective switching or stage...

Page 33: ...ication fluorescence objective into the position on the shallower side of the U SLRE Sliding Revolving Nosepiece U SLRE 1 Raise the revolving nosepiece mount fully by rotating the coarse adjust ment k...

Page 34: ...t can be fabricated by attaching the custom order barrier filter excitation filter and dichroic mirror to the U MF XL Dimension Conditions of Optical Components of Mirror Unit Barrier filter 32 0 1 0...

Page 35: ...ece is installed in correctly Secure it by pushing in the sliding dovetail all the way until the stopper 38 The filter turret or filter slider is incorrectly engaged in the light path Engage them corr...

Page 36: ...s to waver The revolving nosepiece is installed in correctly Secure it by pushing in the sliding dovetail all the way until the stopper 38 The objective is engaged incorrectly in the light path Make s...

Page 37: ...erent eyepieces are used on the left and right Change one eyepiece to match the other so that both sides are of the same type You are not accustomed to parallel opti cal axis When looking into eyepiec...

Page 38: ...Trinocular Field number 22 Tube tilting 30 25 Interpupillary distance adjustment 50 mm to 76 mm Light path selection 3 step switching Binocular 100 Binocular 80 TV photo 20 TV photo 100 2 step switch...

Page 39: ...37 XLFluor Low Power Fluorescence FN 22 2X 340 0 14 20 0 2 40 20X 132 11 4X 340 0 28 28 4 1 20 40X 33 0 5 5 Water immersion impossible Water immersion impossible After using a water immersed objectiv...

Page 40: ...e have your dealer assemble the illuminator or arm The filter turret and cross stage are to be cleaned respectively using the special tools provided with them Note For the detailed assembly procedures...

Page 41: ...on revolving nosepiece WI SNPXLU2 Objective XLFluor2X 340 XLFluor4X 340 RMS adapter WI RMSAD Objective XLUMPlanFl20XW UIS2 UIS objective Microscope frame BX51WIF Clamping band Magnets Magnet support p...

Page 42: ...re at taching the revolving nosepiece see page 20 1 Loosen the revolving nosepiece clamping screw using an Allen screw driver and slide in the revolving nosepiece along the mount dovetail 2 Push the r...

Page 43: ...irection of the fine adjustment dial Fig 46 Direction of the arrow Objective on the back side Opposite direction to the arrow Objective on the front side 5 Remove the objective fit the washer with the...

Page 44: ...Cover Fig 50 Attach the waterproof cover onto the condenser if required The waterproof cover is applicable only to the WI UCD WI DICD and WI OBCD condensers 1 Fit the hole of the waterproof cover in t...

Page 45: ...lamping lever gently back to the original position to clamp the bulb To prevent reduced bulb life or cracking do not touch the bulb with bare hands If fingerprints are accidentally left on the bulb wi...

Page 46: ...ge with the mounting screw holes and and clamp the cross stage by tightening the hex socket screws with the Allen wrench provided with the microscope frame When the waterproof cover is used attach hex...

Page 47: ...ling Table 1 Certified Cord A power supply cord should be certified by one of the agencies listed in Table 1 or comprised of cordage marked with an agency marking per Table 1 or marked per Table 2 The...

Page 48: ...ter sterreichischer Verband f r Elektrotechnik VE Danmarks Elektriske Materialkontroll DEMKO National Standards Authority of Ireland NSAI Norges Elektriske Materiellkontroll NEMKO Asociacion Electrote...

Page 49: ...ON time has exceeded 20 000 hours 2 Lamp does not light sometimes even though the main switch is set to on Except discharge burners 1 3 Illumination flickers when you move the lamp cable or lamp housi...

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Page 52: ...ny 3500 Corporate Parkway P O Box 610 Center Valley PA 18034 0610 U S A One Corporate Drive Orangeburg NY 10962 U S A 491B River Valley Road 12 01 04 Valley Point Office Tower Singapore 248373 31 Gilb...

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