Fig. 37
Fig. 38
Fig. 39
4. Remove the eyepiece from the eyepiece sleeve, look into the sleeve, turn
the polarizer rotation dial ² so that the black interference stripe (Fig. 37)
is darkest, and tighten the clamping knob ³.
5. Engage an objective (as low-magnification as possible) in the light path,
attach the condenser and bring the specimen surface into focus.
6
Observation Method
(Fig. 39)
1. Engage the objective to be used in the light path.
2. When the WI-UCD or U-UCD8 condenser is used, engage the DIC prism
matching the objective in the light path by rotating the turret.
3. Place the specimen on the stage and bring the specimen into focus.
}The contrast may be improved by stopping down the aperture iris dia-
phragm to an optimum aperture.
4. Rotate the polarizer dial @ on the filter turret to obtain optimum contrast
for the specimen. Tighten the clamping knob ² if required.
@
²
@
²
The interference stripe is less clearly visible when the
specimen is thick. In this case, it is recommended to
bring a scratch or like on the bottom of the petri dish to
facilitate the subsequent adjustment operation.
}With the WI-DICD, do not attach the DIC prism.
With the WI-UCD engage a position without DIC prism in the light path.
6. If the condenser has not been centered yet, center it (page 16).
}The interference stripe is not visible clearly if the field iris is focused insuf-
ficiently.
7. Turn the quarter-wave plate rotation ring @ so that the black interference
stripe seen at the center of the eyepiece sleeve’s field of view, then tighten
the clamping knob ². Ignore the short interference stripes in the sur-
roundings in this adjustment.
Since this adjustment renders the field of view dark, observation cannot
be started unless the observation method described in the next item is
employed.
Now the adjustment is complete.
· Attach the eyepiece and objective again to the microscope frame.
· With the WI-DICD, remove it and mount the required DIC prism.
· When an IR cut filter is used, remove it and mount the required filter.
Important
