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NuAire NU-C300R Operation & Maintenance Manual Download Page 3

OM0252 

| 

Rev 4  May/2020

 

Page 3 of 59 

 

NuAire, Inc.

 | 

2100 Fernbrook Lane

 | 

Plymouth, MN 55447

 | 

U.S.A

 | 

ph: 763.553.1270

 | 

fx: 763.553.0459

 | 

tf: 800.328.3352

 | 

www.nuaire.com 

 

NUWIND Centrifuge 

 

 

 

 

 

 

 

TABLE OF CONTENTS 

 

Section No. 1 .......................................................... General Information 

Section No. 2 .......................................................... Models Specifications and Accessories 

Section No. 3 .......................................................... Warranty 

Section No. 4 .......................................................... Shipments 

Section No. 5 .......................................................... Installation Instructions 

 

5.1 ............................................................................ Location 

 

5.2 ............................................................................ Set-up Instructions 

 

5.3 ............................................................................ Certification Testing Methods and Equipment 

Section No. 6 .......................................................... Operating the NU-C300 

 

6.1 ............................................................................ First Start / Powering 

 

6.2 ............................................................................ Operation / Interaction 

Section No. 7 .......................................................... General Maintenance 

Section No. 8 .......................................................... Error Messages, Troubleshooting, Option-Diagnostics & Performance 

Verification 

Section No. 9 .......................................................... Electrical/Environmental Requirements 

Section No. 10 ........................................................ Disposal and Recycle 

Compliance Certificate ........................................... Declaration of Conformity 

 

Annex 1.0 ............................................................... Theoretical Basics of Centrifugation 

Annex 2.0 ............................................................... Accessary Specifications 

 

 

ELECTRICAL SCHEMATICS 

 

BCD-18882 ...................................... NU-C300V / NU-C300V-E 

BCD-18883 ...................................... NU-C300R / NU-C300R-E 

 

Summary of Contents for NU-C300R

Page 1: ...0 328 3352 www nuaire com NUWIND CENTRIFUGE Models NU C300V NU C300R NU C300V E NU C300R E NU C300RF NU C300RF E Operation Maintenance Manual May 2020 Revision 4 120 VAC 60 Hz 230 VAC 50 Hz Manufactur...

Page 2: ...ed one of the finest Laboratory Centrifuges available With proper care maintenance and laboratory procedure this centrifuge will provide you years of productive service Please read this manual careful...

Page 3: ...Set up Instructions 5 3 Certification Testing Methods and Equipment Section No 6 Operating the NU C300 6 1 First Start Powering 6 2 Operation Interaction Section No 7 General Maintenance Section No 8...

Page 4: ...temperature control to further maximize performance The refrigeration system refrigerant used is CFC free to meet regulations of refrigerant use Centrifuges NUWIND 3 0 ventilated and refrigerated mod...

Page 5: ...le the rotor is spinning Do not attempt to neutralize the motorized lid lock Do not lean over the centrifuge during the spin cycle Do not stay within a space of 10 inch around the centrifuge longer th...

Page 6: ...ION used without the safety alert symbol indicates a potentially hazardous situation which if not avoided may result in property damage Safety alert symbol indicates a potentially hazardous situation...

Page 7: ...TU h 5801 BTU h 5801 BTU h Fluid Coolant Charge Not applicable R404A 0 35 kg R404A 0 35 kg Ambient Operating Temperature 20 C 2 C 20 C 2 C 20 C 2 C Temperature adjustment range N A 10 C to 40 C 10 C t...

Page 8: ...750 1 x 750 ml Flat Bottle Set of 2 or 4 NU RX750 Swing Out rotor 4x 750 ml 3 liters One unit NU B750 750 ml buckets Set of 4 NU L750 Lid 750 ml Set of 4 NU 3T2 1 5 2ml MicroTube Set of 2 or 4 NU 3T5...

Page 9: ...and is not assignable or transferable Prior to returning any item for any reason contact NuAire for a Return Authorization Number This number must accompany all returns Any product shipped to NuAire w...

Page 10: ...to 50 C Relative humidity 90 under the dew point 5 2 Unpacking Remove the cardboard packaging made of 2 parts a lid and a bottom The NUWIND centrifuge was packed with great care to prevent transport h...

Page 11: ...de panel to manually open the centrifuge 2 Turn it clockwise until the lid opens up 3 Remove the allen wrench from the hole 4 Remove all the packing foam inside the bowl 5 Remove the buckets and put t...

Page 12: ...ge It is recommended that no less than two people are present to manually lift the centrifuge onto the laboratory bench 5 4 Electrical environment The centrifuge requires must be 230V 50Hz or 120V 60H...

Page 13: ...nc 2100 Fernbrook Lane Plymouth MN 55447 U S A ph 763 553 1270 fx 763 553 0459 tf 800 328 3352 www nuaire com 6 0 Operating the NU C300 6 1 First Start Powering The first start must fulfill the condit...

Page 14: ...the rotor on the drive shaft 4 A click indicates that the rotor is locked 5 Clean and lubricate the pins by depositing a thin film of the food grease supplied 6 1 2 Position the buckets Swing out roto...

Page 15: ...or Each position on the rotor must be equipped with its bucket Do not use a rotor without its complete set of four buckets Maximum loads according to the type of rotor are specified in the annex The c...

Page 16: ...63 553 0459 tf 800 328 3352 www nuaire com 6 1 7 Quick start up Before using the centrifuge make sure it has been properly installed 6 2 Operating 6 2 1 Settings This section is accessible through the...

Page 17: ...grams are available from 01 to 99 The program can be modified when the protection icon is an open lock Edit the title screen by pressing on the title Enter the new title Save the new title by pressing...

Page 18: ...the desired parameter by pressing the icon Acceleration slope from 1 to 9 1 slowest acceleration 9 fastest acceleration Braking slope from 0 to 9 0 no braking at all 1 slowest deceleration 9 fastest...

Page 19: ...328 3352 www nuaire com 6 2 4 Set speed in RPM or RCF Visual Instruction Control Access the speed setting key Press on the icon RPM to modify the unit from RPM to RCF Enter the RCF g value desired Con...

Page 20: ...ked to prevent any modifications of the settings Visual Instruction Control Select the program to lock Press on the open lock for 4 seconds The lock is now closed The program is now protected Note To...

Page 21: ...e Use a program Turn on the centrifuge The switch is in the lower right portion Any user can access a program user admin service tech Visual Instruction Control Select the program you want to use Load...

Page 22: ...Open the lid 6 2 8 Open after an electric power failure Emergency Stop This operation must be performed by a person trained in centrifugation related risks In case of power failure the lid cannot be o...

Page 23: ...or to centrifugation allows to cool the centrifuge down to 4 degrees Celsius so it is ready for temperature sensitive samples Visual Instruction Control Access to the list of programs by pressing this...

Page 24: ...at a colder temperature Visual Instruction Control Access to the list of programs by pressing this icon Select the pre warming program This program is set to warm the centrifuge up to 20 C during a 20...

Page 25: ...er Preference Visual Instruction Control Access the User Preferences menu via the following icons Type of speed displayed RPM or RCF G force Type of temperature displayed Celsius or Fahrenheit Frequen...

Page 26: ...763 553 0459 tf 800 328 3352 www nuaire com 6 2 13 Type of Speed displayed RPM OR RCF Visual Instruction Control Select the unit you want displayed by default RPM Rotation Per Minute RCF Relative Cent...

Page 27: ...e Celsius F Degree Fahrenheit Confirm 6 2 15 Frequency of lubrication Visual Instruction Control Select how many cycles you want between each lubrication reminder No reminder Not recommended 100 cycle...

Page 28: ...ount of hours the centrifuge has been used COLD MODULE RUNNING HOURS Amount of hours the refrigeration has been running COLD MODULE STARTS Number of time the refrigeration has started compressor LID O...

Page 29: ...function activates a beep when an error message appears ON Upon error the signal is triggered Pressing the key stops the signal OFF No signal upon error This is the default 6 2 22 Post Cool This func...

Page 30: ...int the rotor and accessories are cooled 6 2 24 Temperature Display Mode Allow for display of air or rotor temperature AIR The temperature displayed is the temperature of the air in the bowl This is t...

Page 31: ...mation on the Software and Hardware of the centrifuge SERIAL NUMBER Unique identification number of the centrifuge Centrifuge fw Software version of the microcontroller Display fw Software version of...

Page 32: ...ns 7 1 Care and Cleaning 7 1 1 Authorized maintenance products Users should not use cleaning or decontamination methods different from those recommended by the manufacturer as they may cause damage to...

Page 33: ...ons Make sure to set the greasing reminder in the User Preferences menu To prolong the life of accessories you can then place a thin film of silicone spray or lanolin Rotors buckets inserts can be aut...

Page 34: ...of their mechanical strength These limits are marked on the accessories Visual Instruction Control When there is only 2 000 cycles left before the end of the theoretical life time of the rotor this sc...

Page 35: ...ched the end of their life cycle is prohibited These components must be replaced 7 1 8 Storage of accessories Do not store the accessories on a wire rack Oxidation build up may appear and show prematu...

Page 36: ...ceed in the same way as for the 15 gram weight 9 The low threshold is then determined e g 520 10 Open the lid and remove the 25 gram weight Average 11 Press on the Next button to average the setting e...

Page 37: ...high Make sure the samples can be cooled to down to a temperature between 4C and 37C during 1 hour The refrigeration doesn t work Contact a Certified Technician The temperature probe is malfunctionin...

Page 38: ...le to be opened and run normally again 11 WRONG SPEED SIGNAL The speed sensor is not working properly The board with tachometer accelerometer is working improperly The connection to these parts is not...

Page 39: ...ISSUE 2 possible situations Signal permanently lost Impossible to clear the Error and open the lid 30 minute safety timer is running Wait for 30mn before being allowed to open the lid Speed signal re...

Page 40: ...It depends on the nature of the electricity supply and its overvoltage protection means For example in CAT II which is the category used for instruments in installations supplied from a supply compara...

Page 41: ...OM0252 Rev 4 May 2020 Page 41 of 59 NuAire Inc 2100 Fernbrook Lane Plymouth MN 55447 U S A ph 763 553 1270 fx 763 553 0459 tf 800 328 3352 www nuaire com...

Page 42: ...ector which includes this centrifuge must be disposed of with household waste For easy identification the appliances are marked with the symbol displaying a crossed out dustbin Centrifuges that are no...

Page 43: ...on samples The effective force increases with the square of the rotational speed and distance from the axis of rotation This force known as g force is quantified as the number of g applied to the sam...

Page 44: ...nuaire com 1 2 Nomogram The nomogram graphically connects by a line segment a speed to the relative centrifugal force RCF as a function of the radius of rotation selected In the example above the spe...

Page 45: ...Inc 2100 Fernbrook Lane Plymouth MN 55447 U S A ph 763 553 1270 fx 763 553 0459 tf 800 328 3352 www nuaire com 1 3 Logbook Example of table to monitor the use of the centrifuge and accessories Date O...

Page 46: ...force NU C300 Ventilated 3500 rpm 2750 xg NU C300 Refrigerating 3500 rpm 2750 xg Buckets Part number NU B1000 Description 1 000 ml bucket Set of 4 Maximum speed 3500 rpm Maximum RCF 2750 xg Maximum ca...

Page 47: ...is the distance between the rotor shaft and the bottom of the insert holes when the bucket is spinning in a horizontal position during centrifugation Microplate carrier Part number NU BXDW4 Descripti...

Page 48: ...be loaded evenly Allowed Forbidden Loading the rotor The rotor must be loaded evenly Allowed Forbidden Note A message appears when choosRX1000 rotor 2x NU B1000 loaded buckets inserts loaded tubes 2 N...

Page 49: ...lated 4500 rpm 4527 xg NU C300 Refrigerated 4600 rpm 4731 xg Accessories Part number NU B750 Description Round Bucket 750 ml Set of 4 Volume 750 ml Maximum speed 4600 rpm Maximum RCF 4730 xg Maximum c...

Page 50: ...rotor NU RX750 Set of 2 OR 4 Maximum speed 4600 rpm Maximum RCF 4564 xg Capacity standard Microplates 4 plates per bucket total of 16 Capacity Deep well blocks 1 block per bucket total of 4 Maximum c...

Page 51: ...A ph 763 553 1270 fx 763 553 0459 tf 800 328 3352 www nuaire com Possible Configurations Loading the buckets Each bucket must be loaded evenly Allowed Forbidden Loading the rotor The rotor must be loa...

Page 52: ...ntrifuge performance Maximum speed Maximum centrifugal force NU C300 Ventilated 4500 rpm 3531 xg NU C300 Refrigerated 5100 rpm 4537 xg Possible Configurations Loading the buckets Allowed Not allowed L...

Page 53: ...g Maximum radius 102 mm Angle Rotor NU RA16 5 Rotor Lid included Part number NU RA16 5 Description 16 x 5 ml Angle rotor Maximum capacity 16x 5 ml micro tubes Maximum load 16 x 36 gr Micro tube tilt 4...

Page 54: ...351 xg Maximum radius 106 mm Accessoriess Tubes adapters Set of 6 Part number Radius Capacity per insert Total capacity Rotor AFI 100R80 OAK mm 1x 80 ml Tube AOR Advanced Oak Ridge 6 AFI 100R50C mm 1x...

Page 55: ...I C200 Ventilated 4500 rpm 3169 xg AFI C200 Refrigerating 4800 rpm 3606 xg Maximum radius 140 mm Items included 30 Tube holders 30 Wells for round bottom tubes Accessories for NU RA30 15 Cups Part Num...

Page 56: ...g ClickSpin system Maximum rotor speed 15500 rpm Maximum centrifugal force of the rotor 26620 xg Centrifuge performance Maximum speed Maximum centrifugal force AFI C300 Ventilated 14000 rpm 22132 xg A...

Page 57: ...tube tilt 45 Fastening ClickSpin system Maximum rotor speed 16000 rpm Maximum centrifugal force of the rotor 27720 xg Centrifuge performance Maximum speed Maximum centrifugal force AFI C200 Ventilated...

Page 58: ...OM0252 Rev 4 May 2020 Page 58 of 59 NuAire Inc 2100 Fernbrook Lane Plymouth MN 55447 U S A ph 763 553 1270 fx 763 553 0459 tf 800 328 3352 www nuaire com...

Page 59: ...OM0252 Rev 4 May 2020 Page 59 of 59 NuAire Inc 2100 Fernbrook Lane Plymouth MN 55447 U S A ph 763 553 1270 fx 763 553 0459 tf 800 328 3352 www nuaire com...

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