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ELC-03XS User Manual

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version 2.2

page 8

3.3.

Front Panel View of the ELC-03XS Amplifier

Figure 1: ELC-03XS front panel view

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Summary of Contents for ELC-03XS

Page 1: ...SAL AMPLIFIER for EXTRA INTRACELLULAR RECORDING SINGLE CELL STIMULATION and ELECTROPORATION VERSION 2 2 npi 2011 npi electronic GmbH Hauptstrasse 96 D 71732 Tamm Germany Phone 49 0 7141 9730230 Fax 49...

Page 2: ...of the ELC 03XS Amplifier 8 3 4 Description of the Front Panel 9 4 Description of the Rear Panel 19 Grounding 21 5 Setting up the ELC 03XS 22 6 Passive Cell Model 23 6 1 Cell Model Description 23 6 2...

Page 3: ...is to be operated only at 115 230 Volt 60 50 Hz AC Please check for appropriate line voltage before connecting any system to mains Always use a three wire line cord and a mains power plug with a prot...

Page 4: ...is particularly well suited for long term recording with little damage to the recorded neuron Nunemaker et al 2003 It can be used both for somatic and axonal recording Khaliq Raman 2005 Even subcellul...

Page 5: ...rdings are specialized on the recording of the potentials or currents generated by the neurons under investigation If these recording methods are combined with dye injection electroporation stimulatio...

Page 6: ...pacity compensation controls for VC and CC mode all controls Bridge balance CC cap comp offset holding controls are calibrated 10 turn potentiometers The ELC 03XS is capable to record extracellulary D...

Page 7: ...he GATE BNC is turned off Computer control of the mode of operation In the EXT position of the MODE SELECT switch all MODEs OF OPERATION can be selected by TTL signals connected to the rear panel see...

Page 8: ...______________________________________________________________________________ ___________________________________________________________________________ version 2 2 page 8 3 3 Front Panel View of th...

Page 9: ...r 4 MANUAL push button 5 FREQUENCY potentiometer 47 AMPLITUDE potentiometer and 48 DURATION potentiometer 2 mode switch 5 position switch to set the mode of PENETRATION CC The BUZZ circuit is activate...

Page 10: ...The duration is dependent on the setting of the DURATION potentiometer but independent from how long the button is pushed or a REMOTE switch is pressed 12000 8000 4000 0 4000 8000 12000 time potentia...

Page 11: ...A connector BNC connector providing a voltage proportional to the current at the electrode with a fixed scaling of 100 mV nA This current signal is not filtered CURRENT set unit The CURRENT set unit c...

Page 12: ...connector for the current stimulus in CC BR or in CCx10 mode scaling 1 nA V or 10 nA V respectively 15 GATE TTL connector BNC connector for gating the potential step in VC VCx10 mode or the current st...

Page 13: ...COMP for compensation of the voltage drop at the SERIES RESISTANCE For instance when for calculating I V curves do not use the potential values recorded from the POTENTIAL OUTPUT mV BNC connector 27 U...

Page 14: ...to the headstage is disabled The amplifier is automatically switched to CC OFF mode and only potential measurement works POTENTIAL OUTPUT FILTER The POTENTIAL OUTPUT FILTER consist of 24 HIGHPASS Hz a...

Page 15: ...CAPACITY COMPENSATION works only for the electrode capacity not for the capacity of the cell membrane Therefore capacitive transients are always present when square shaped pulses are applied in VC mo...

Page 16: ...be set to 20 kHz and the HIGHPASS 24 to DC With an amplifier with switchable headstage ELC SWI the electrode resistance test works also in enhanced x100 current mode Note If the ELC 03XS is equipped...

Page 17: ...potential since these values include also the additional voltage generated by SERIES RESISTANCE COMP for compensation of the voltage drop at the SERIES RESISTANCE For instance when for calculating I...

Page 18: ...that the BRIDGE circuit is active 44 BRIDGE MODE switch Switch for activating the BRIDGE circuit The active circuit is indicated by 43 45 CURRENT nA display Display for the current at the electrode i...

Page 19: ...or BNC connector providing a voltage monitoring the position of the CURRENT OUTPUT SENSITIVITY switch 1 V to 7 V 1V STEP 3 LP FILTER POTENTIAL connector BNC connector providing a voltage monitoring th...

Page 20: ...istance test remotely 9 BR connector BNC connector for remote control of the bridge mode A TTL HI 5 V signal can be connected here to select the bridge mode remotely 10 OFF connector BNC connector to...

Page 21: ...is also monitored at 20 see below 18 COMMAND MONITOR TTL connector BNC connector providing a TTL 5V signal synchronous to the 10 mV test pulses 19 SEAL TEST INPUT TTL connector Starts seal test remote...

Page 22: ...tage to the HEADSTAGE connector 1 Figure 1 at the ELC 03XS Connect a cell model see chapter 6 Connect a digital analog timing unit or a stimulation device to STIMULUS INPUT or to GATE TTL if you inten...

Page 23: ...is broken or if something is wrong with the electrodes or holders etc Figure 4 ELC MOD passive cell model The passive cell model consists only of passive elements i e resistors that simulate the resis...

Page 24: ...200 M and 100 pF CELL 2 5 In GROUND lower position the electrodes are connected to ground via a 1 k resistor In SEAL upper position are connected to a 1 G resistor simulating the formation of a GIGASE...

Page 25: ...t switch 5 Figure 5 to SEAL position The 1 G resistor simulates the SEAL resistance when forming a GIGASEAL in patch clamp experiments Simulation of intracellular recording Intracellular recordings ca...

Page 26: ...for the electrode holder REF Connector for the reference electrode differential headstage only GND Ground connector TYPE Type of amplifier BA Bridge amplifier ELC ELC amplifier R FB Value of feedback...

Page 27: ...ecording electrode The zero current of this unit is tuned with the BIAS current potentiometer 49 Figure 1 The tuning procedure should be performed regularly at least once a month since the BIAS curren...

Page 28: ...so chapter 4 If the switch is set to ON the current range is extended to 12 A This mode is indicated at the headstage by an additional LED x100 ON see Figure 10 Important In the VC x10 range which is...

Page 29: ...er 33 Figure 1 8 1 Recordings with the Differential Headstage optional Extracellular measurements are mostly done in slices or in vivo in noisy environments where distortions of the recorded signal ca...

Page 30: ...ellular metal electrodes is simple The electrode is advanced into the region where the recordings will be made using a micromanipulator and the signals are filtered and amplified see chapter 5 in Lall...

Page 31: ...mulus with a TTL signal linked to the GATE TTL BNC connector Electroporation with Current Electroporation can be done using the stimulation procedure but usually the applied current is much higher and...

Page 32: ...ING CURRENT potentiometer and the HOLDING CURRENT polarity switch Apply stimuli to the cell using the STIMULUS INPUT 1 nA V BNC connector Voltage Clamp Recording The ELC 03XS can also be used like a w...

Page 33: ...Isope P 2000 Combining loose cell attached stimulation and recording J Neurosci Methods 103 199 208 Bureau I Shepherd G M G Svoboda K 2004 Precise Development of Functional and Anatomical Columns in t...

Page 34: ...28 10017 10022 Transfection using ELC amplifiers Stan A Pielarski K N Brigadski T Wittenmayer N Fedorchenko O Gohla A Lessmann V Dresbach T Gottmann K 2010 Essential cooperation of N cadherin and neu...

Page 35: ...ge 0 30 pF ten turn control BIAS range 100 pA ten turn control Bridge balance 0 100 M adjustable with ten turn control Electrode resistance test Sensitivity 1 mV M application of square current pulses...

Page 36: ...ay current 3 digits XX XX nA Inputs Input impedance analog 100 k Input range 12 V Input impedance digital TTL 10 k Input range TTL 0 5 V Current stimulus input CC via BNC connectors sensitivity 1 nA V...

Page 37: ...t unit 13 CURRENT set unit 11 Differential Headstage optional 29 ELC 03XS Components 6 Optional Accessories 6 electrical connections 22 Electroporation 31 Experiments 29 Extracellular Stimulation 31 E...

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