ELC-03XS User Manual
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version 2.2
page 26
7. Headstage
The ELC-03XS comes with a headstage for connecting suction electrodes for loose-patch
clamp or whole cell recordings and / or stimulation or electroporation, respectively or sharp
electrodes for intracellular or extracellular recordings. The use of metal electrodes is possible
as well.
A differential (miniature) headstage (see
Optional accessories
in chapter 3.1) for
measurements
in vivo
is also available (see also chapter 8.1 and contact npi for details).
7.1. Headstage
Elements
Figure 7: ELC-03XS headstage
P
EL
BNC connector for the electrode holder
REF
Connector for the reference electrode (differential headstage only)
GND Ground
connector
TYPE
Type of amplifier, BA
Bridge amplifier, ELC
ELC amplifier
R FB
Value of feedback resistor, 10M: 10 M
, 100 M: 100 M
Headstage cable to amplifier
Mounting plate
The electrode filled with electrolyte is inserted into an electrode holder (optional) that fits into
the BNC connector of the headstage or into an electrode holder adapter. The electrical
connection between the electrolyte and the headstage is established using a carefully
chlorinated silver wire. Chlorinating of the silver wire is very important since contact of silver
to the electrolyte leads to electrochemical potentials causing varying offset potentials at the
electrode, deterioration of the voltage measurement etc. (for details see Kettenmann and
Grantyn (1992)). For optimal chlorinating of sliver wires an automated chlorinating apparatus
(ACl-01) is available (contact npi for details).
