Nikon Epiphot 300 Manual Download Page 53

Page: 53 / 80

15. Optical System

• Use CF objectives always in combination with CF eyepieces.
• Nikon's 50x or higher magnification objectives (except ELWD) are equipped with a

safety mechanism. However, the top lens of each of these objectives slightly protrudes

beyond the metal rim around it. Carefully rotate the revolving nosepiece to which these

high magnification objectives have been mounted. Do not hit them against the sample or
the like.

• CF PL projection lenses are designed only for use in photomicrography. Do not use them

as eyepieces.

• Eyepieces tend to collect dirt or dust. Both may appear as shadows or impair image

contrast or resolution. Keep eyepieces always clean.

• Leave the analyzer slider, the polarizer slider and the reticle slider in their respective

ports to prevent dust from attaching to the optical system in the microscope base. (Or use

their dust-tight sliders for the same purpose.)

�

::JJ

-t

Summary of Contents for Epiphot 300

Page 1: ... t l Inverted Metallurgical Microscope EPIPHOT 300 ...

Page 2: ...ill use Nikon s inverted metallurgical microscope EPIPHOT 300 Before using the microscope read this manual from cover to cover to acquire flawless knowledge of its safe operation Such knowledge will enable you to make the best use of this microscope After reading this booklet keep it as reference material ...

Page 3: ...est Nikon representative 3 Heat at the Lamphouse The lamp and the lamphouse are very hot during and right after the illumination Be careful not to get burned 0 The warning label on the back of the I2V JOOW lamphouse 2 is a reminder for the above caution Do not touch the lamphouse within 30 minutes after turning off the power switch of the power supply unit Do not bring the following near the lamph...

Page 4: ... of other light sources may result in malfunction Specified Lamps Specified Lamphouse Adapters Halogen lamp 12V IO0W LONGLIFE 12V I00W lamphouse OSRAM HLX64623 or PHILIPS 7724 adapter for XJOOS Halogen lamp 12V 50W 12V SOW lamphouse for OSRAM HLX64610 or PHILIPS 7027 U epi 10 Specified Lamphouses 12V 100W lamphouse 2 with pre centered socket 12V 50W lamphouse adapter for U epi 10 6 Use of a Mercur...

Page 5: ... lamp Turn off the power switch of the power supply unit and unplug the power cable off the AC IN receptacle when replacing the lamp Do so to avoid electrical shocks to you or damage to the microscope Turn off the power switch of the control box and unplug the power supply cable off the control box when replacing the fuse 9 Short Circuit and Abnormal Heating Do not spill water onto the microscope ...

Page 6: ...ron ment in which it is used or kept in custody Use the microscope in an adequate environment as an environment inappropriate to its operation may be responsible for malfunction For more detail see Where to Install on page 5 12 Cautions when Carrying the Microscope This microscope is a precision instrument Handle it with enough care Do not give shocks or impacts to it The accuracy of some parts of...

Page 7: ... When moving the microscope on the desk do not drag it Lift it and put it down gently where it is to be placed again 13 Serial Numbers of the Microscope and Key Component The serial numbers of the microscope its eyepiece tube and of the control box should be identical If this microscope is used together with an eyepiece tube and a control box each with a serial number different from that of the mi...

Page 8: ...6 4 Polarizer Slider 37 5 Analyzer Slider 38 6 First Order Red Compensator Slider 38 7 Mirror Bwcks 39 8 Optical Path Changeover Slider 40 9 Eyepiece Tube 41 10 ZoomDial 41 11 FocusingDevice 42 12 Rectangular Mechanical Stage 43 13 Grain Reticle 44 14 Micrometer Reticle 45 15 Optical System 46 VI PHOTOMICROGRAPHY 47 1 Film Loading and Unloading 47 2 Exposure Mode Table 51 3 OperationalDetails 56 4...

Page 9: ... x5 film holder adapter 35 mm LARGE changeover lever Zoom dial Stage ring Mirror block changeover lever Analyzer slider Dust tight slider Rectangular mechanical stage Anti glare NOB filter insertion removal lever Polarizer slider Dust tight slider Lamphouse Control box 08 PHOTO SIDE changeover lever Stage lateral movement knob Stage longitudinal movement knob Fine focus knob Coarse focus knob Coar...

Page 10: ...er pocket Side port Reticle slider Dust tight slider Aperture diaphragm dial Field diaphragm dial Mirror block port DX cable 35 mm dark box 35 mm dark box attaching mount Lamp intensity control dial AC IN connector Lamphouse cable connector Ex control connector Ex control cable Ex control connector 2 z 0 s m z 0 C m ...

Page 11: ... ISO keys DX mode display lamp ISO value display Fuse 1A 250V Power switch Power cable Receptacle for hand held switch 3 CHECK display Exposure time display RST reset key Film end LED WIND key EXP ADJ exposure adjustment value display DX cable Plug insertion detaching button Signal cable ...

Page 12: ... advance mode switch Rewind knob Rewind crank Attaching detaching button l 1 ____________ DX_C_ o_nn_e_ct_or NFX 35 Film advance indicator Data mask IN OUT lever Rewind knob Rewind crank Attaching detaching button DX cable connector I z 0 s m z t C 0 m 4 ...

Page 13: ...s used as a light source or when the metal halide light source is used Insert the lamphouse adapter into the sleeve in the back of the microscope Three cross recessed head screws are supplied with the adapter Insert two of them into the two screw holes in the upper part of the adapter Tighten these two screws with a screwdriver The two tightened screws provide the adapter with enough strength with...

Page 14: ...gonal screwdriver Fit the circular dovetail of the nosepiece to the corresponding mount in the microscope base Ensuring that the outer circumference of the nosepiece s dovetail is in contact with the claws on the mount tighten the clamp screw with the supplied centering tool Stage The stage attachment base of the microscope has two pins Place the stage onto the attachment base so that the two pins...

Page 15: ...onding protrusions of the eyepiece sleeve After inserting the eyepiece with a built in mask confirm that this mask is not slanted I I I j Rubber eyeguards for binocular eyepieces option Insert each eyeguard into the groove in the outer circumference of the eyepiece Rotation clamp rings The rotation clamp ring is attached to the eyepiece sleeve to prevent the rotation of the eyepiece When removing ...

Page 16: ...the revolving nosepiece Mount the objectives in such a way that the magnification of the objectives mounted increases with the clockwise rotation of the nosepiece when it is viewed from above Ex 150x Power Supply Unit For 100 120 V area Use only the power supply unit model PSM 1120 provided by Nikon Corporation For 220 240 V area Use only the power supply unit model PSM 2120 provided by Nikon Corp...

Page 17: ...ntrol box Ni Cd battery Do not dispose of used or disused batteries Return them to your nearest Nikon representative Turn off the power switch of the control box before connecting the cables Connect the control box to the microscope base with the signal cable Push the plug insertion detaching button of the signal cable connector to insert the plug of the signal cable straight into the receptacle m...

Page 18: ... bottom of the dark box The NFX 35 dark box doesn t work when its mode switch is set at A When the attached data back is not used tum the mask IN OUT lever of the NFX 35 dark box to OUT Align the index of the dark box with that of 35 mm dark box attaching mount of the microscope base Rotate the dark box in the arrow direction to the limit to attach the dark box When detaching the dark box push its...

Page 19: ...If an additional photomicrographic attachment is required besides the photomicrographic system built in the microscope base mount one to the side port Fix the viewfinder the FM mount the dark box and other components to the main unit of the photomicrographic attachment by referring to its instruction manual Detach the cap from the side port tube Insert an PL projection lens into the side port tube...

Page 20: ...apter Use the hexagonal screwdriver to loosen the two side port tube clamp screws at the base of the side port Pull out the side port tube Insert the direct C mount adapter into the side port Tighten the above two screws with the hexagonal screwdriver to fix this adapter Use of the Other Light Sources Use of 12V 50W halogen lamp CAUTIONS Use 12V 50W halogen lamps specified in Electrical Specificat...

Page 21: ...the Metal Halide Light Source Insert the end of the fiber into the fiber adapter until it reaches the limit Fix this end of the fiber with the clamp screw supplied with the fiber adapter Do not apply excessive force to the clamp screws Insert the fiber attached fiber adapter into the sleeve in the back of the microscope Fix the adapter with the clamp screw supplied with the sleeve Refer to the ins...

Page 22: ...inocular eyepieces with 100 light output 3 Tum on the power switch of the power supply unit Rotate its lamp intensity control dial to CTRL Set the lamp intensity control dial of the microscope base at about 6V 4 If a Nomarski prism the polarizer and the analyzer are in the optical path remove them from the optical path 5 Fully open the aperture and field diaphragms Fully open the aperture diaphrag...

Page 23: ...Place a flat high reflectance sample on the stage Face its surface to be observed downward 9 The eyepiece with a built in mask is in the right ti eyepiece sleeve Rotate the diopter correction ring of this eyepiece to focus on its mask Figure right 10 View the sample through the right eyepiece While so doing rotate the coarse focus and fine focus knobs to focus on the sample Stop down the field dia...

Page 24: ...upillary Distance Adjustment View the viewfield through the binocular eyepieces While so doing adjust the interpupillary distance until the right and left viewfields coincide Interpupillary distance adjustment causes the eyepieces to rotate However the right eyepiece sleeve contains a mechanism which keeps the eyepiece in it from being rotated during interpupillary distance adjustment If the eyepi...

Page 25: ...ening in the stage ring almost right above the top of that objective 7 Place a flat high reflectance sample on the stage Face its surface to be observed downward 8 Rotate the coarse focus and fine focus knobs to focus on the sample Stop down the field diaphragm to make the field diaphragm image clearly visible This makes focusing easier 9 Loosen the eyepiece tube clamp screw to tum the eyepiece tu...

Page 26: ...block changeover lever to put the brightfield mirror block in the optical path 2 Push both the OB PHOTO SIDE and OB PHOTO changeover levers to provide the binocular eyepieces with 100 light output 3 Turn on the power switch of the power supply unit Rotate its lamp intensity control dial to CTRL Set the lamp intensity control dial of the microscope base at about 6V 4 If a Nomarski prism the polariz...

Page 27: ... eyepiece viewfield Rotate the centering tools to center the field diaphragm until the the field diaphragm image and the eyepiece viewfield are concentric I Each of the centering tools reaches the limit when it is rotated clockwise ________________________________ _ in the screw hole Do not rotate it further with excessive force If I 1 rotated forcibly it may be damaged I 20 ...

Page 28: ...h to the IOx objective Rotate the stage movement knobs to locate the center of the opening in the stage ring almost right above the top of that objective 7 Place a flat high reflectance sample on the stage Face its surface to be observed downward 8 Rotate the coarse focus and fine focus knobs to focus on the sample Stop down the field diaphragm to make the field diaphragm image clearly visible Thi...

Page 29: ...phragm image then become visible on the objective s exit pupil Rotate the centering tools to center the aperture diaphragm until the aperture diaphragm image and the exit pupil are concentric Objective s exit pupil Aperture diaphragm image I 0 a m a 0 z 22 ...

Page 30: ...zer analyzer first order red compensator Nomarski prisms universal quintuple nosepiece and DIC objectives Simplified polarization set also required Set includes polarizer l analyzer and first order red compensator Available Microscopy Brightfield Darkfield Differential interference contrast Simplified brightfield polarization Sensitive brightfield polarization See page p 24 See page p 27 See page ...

Page 31: ...ensator are in the optical path remove them from the optical path During nonnal observation Nomarski prisms SIA S1B S1C or S1E can be left in the optical path But remove them when photomicrography is perfonned or when a sample with muchfiner details is observed 3 Turn on the power switch of the power supply unit Rotate its lamp intensity control dial to CTRL Set the lamp intensity control dial of ...

Page 32: ...s to focus on the sample OT I 12V 100W lamphouse M i I 9 Switch to the desired objective and focus on the sample 12V 50W lamphouse 1 Nikon s 50x or higher magnification objectives except ELWD are 1 equipped with a safety mechanism However the top lens of each of these objectives slightly protrudes beyond the metal rim around it When rotating the revolving nosepiece to which these high magnificatio...

Page 33: ... the image of the stopped down diaphragm is slightly larger than the actual dimensions of thefilm format 12 Turn the aperture diaphragm dial to open or stop down the aperture diaphragm properly The aperture diaphragm is used to adjust the illumination system s numerical aperture and plays an important role in determining image resolution contrast and depth of focus Detach one of the eyepieces from...

Page 34: ...e analyzer the first order red compensator and the reticle are in the optical path remove them from the optical path During normal observation Nomarski prisms SJA SJB SJC or SJE can be left in the optical path But remove them when photomicrography is pe formed or when a sample with muchfiner details is observed 3 Tum on the power switch of the power supply unit Rotate its lamp intensity control di...

Page 35: ...ness with ND filters and the lamp intensity control dial When the quintuple brightfield revolving nosepiece is used it is impossible to perform dark field microscopy Changing over between Brightfield and Darkfield Microscopy 1 Push the anti glare ND8 filter insertion removal lever to put this filter in the optical path 2 Changeover from brightfield to darkfield microscopy G Pull the mirror block c...

Page 36: ...sepiece Rotate the nosepiece to put the l0x objective in the opticalpath 8 Place a sample on the stage Face its side to be observed downward View the sample through the binocular eyepieces While so doing rotate the coarse focus and fine focus knobs to focus on the sample 9 Switch to the desired objective and focus on the sample I Nikon s SOx or higher magnification objectives except ELWD are _____...

Page 37: ...sed Nicols position The direction of the analyzer has been factory adjusted 14 Adjust the brightness with ND filters and lamp voltage adjustment The resulting dark background without thefirst order red compensator in the optical path produces an interference image similar to that obtained through brightfield phase contrast observation The gradual rotation of the polarizer changes the background to...

Page 38: ...e the binocular eyepieces with 100 light output 8 Rotate the coarse focus knob to lower the revolving nosepiece Rotate the nosepiece to put the 1Ox objective in the optical path 9 Place a sample on the stage facing its side to be observed downward View the sample through the binocular eyepieces and while so doing rotate the coarse focus and fine focus knobs to focus on the sample 10 Switch to the ...

Page 39: ...ical path Align the dot index on the polarizer rotation ring with the triangle index of the polarizer slider to attain the crossed Nicols position This enables simplified brightfield polarization microscopy to be performed 14 Adjust the brightness with ND filters and the lamp intensity control dial 15 Put the first order red compensator in the optical path It is now possible to carry out microscop...

Page 40: ...reduces light to 1 8 Anti glare filter for Anti glare ND8 brightfield microscopy filter Anti glare ND8 Acts as ND8 in insertion removal brightfield transparent glass in darkfield lever built in its microscopy slot Green Contrast adjustment for GIF monochrome Filter holder interference photomicrography The above filters are used as follows Filter holder Filter holders are inserted into the lamphous...

Page 41: ...ush this lever to put the ND8 filter in the optical path When the use of this filter is not needed pull this lever to the limit Push or pull the NDS insertion removal lever always to the limit ND8 filter in the optical path I 0 1J m i5 z 0 m c D I 34 ...

Page 42: ...ical aperture N A of the illumination system and plays an important role in determining image resolution contrast and depth of focus Since resolution is reduced when the aperture diaphragm is stopped down more than necessary do not stop it down to 60 or below of the N A of the objective in the optical path except when a special sample is observed The aperture diaphragm image may not be visible whe...

Page 43: ...lluminated for observation Ifthe sample is illuminated wider than necessary because thefield diaphragm is not adjusted properly stray light will enter the viewfield to generate aflare and reduce the image contrast Correct adjustment of this diaphragm is therefore very important especially for photomicrography As a general rule thefield diaphragm is to be stopped down until the image of the stopped...

Page 44: ...olarizer rotation ring positioned on the left side of the microscope for easier operation of the polarizer When inserting the polarizer slider face the side of the polarizer rotation ring with the dot and bar indexes toward the front of the microscope When changing over to brightfield or darkfield microscopy pull the polarizer slider to the first click stop to put its empty hole in the optical pat...

Page 45: ... or differential interference contrast microscopy insert the first order red compensator slider and the polarizer slider into the polarizer port until it reaches the limit This puts both the first order red compensator and the polarizer in the optical path Figure below I In altering the compensation range in differential interference contrast microscopy or in changing over from sensitive polarizat...

Page 46: ... Push in the mirror block changeover lever to put the mirror block on the right in the optical path Pull out the lever to put the mirror block on the left in the optical path With the front of the microscope facing you attach the brightfield mirror block on the right hand half of the mount inside the mirror block port Attach the darkfield mirror block on the left half of the same mount Never attac...

Page 47: ...hree optical path changeover levers 0B PHOTO SIDE OB PHOTO and 35 mm LARGE to divide the total light output as illustrated below 1 Push or pull the optical path changeover levers always to the limit I Pull out 100 20 20 20 I 0 m rJ I 0 2 0 TI m c rJ I 40 ...

Page 48: ...m Dial The zoom dial is used to operate the zoom lens built in the microscope Zoom magnification ranges from 0 8x to 2x Tum this zoom dial to set the desired magnification within the above range Intermediate magnification set by the zoom dial is effective both to microscopic observation and photomicrography 35 mm film or large format film It is not necessary to adjust the magnified dimensions of t...

Page 49: ...fine focus knob moves the nosepiece 0 1 mm The minimum reading of the scale on the fine focus knob moves the nosepiece 1 µm Rotation of the coarse focus torque adjustment ring adjusts the tension felt in the rotation of the coarse focus knob Rotating this adjustment ring counter clockwise increases this tension Coarse focus torque adjustment ring Never rotate simultaneously the right and left focu...

Page 50: ...r can therefore angle the handle to positions convenient for him to move the stage He also can shift his hand quite easily from the coarse focus and fine focus knobs to the stage movement knobs and vice versa Two stage rings each with a central opening are available for samples of different dimensions One is a J 20 opening and the other a J 40 opening ...

Page 51: ... the pattern second to the next low numbered one Grid for testing non ferrous inclusion This grid can be used as a simplified length scale It has 20 vertical and 20 horizontal lines The spacing between any two vertical or horizontal lines is 0 5 mm on the reticle surface This spacing when viewed through the binocular eyepieces with the 1Ox objective in the optical path represents 0 05 mm on the sp...

Page 52: ... be printed in on a photograph adjust the position of the slider in the direction in which it slides until the micrometer is located within the viewfinder photomask of the photomicrographic attachment When using a TV camera watch the monitor screen and adjust the position of the slider in the direction in which it slides until the micrometer is located within the projected area Micrometer changeov...

Page 53: ...objectives have been mounted Do not hit them against the sample or the like CF PL projection lenses are designed only for use in photomicrography Do not use them as eyepieces Eyepieces tend to collect dirt or dust Both may appear as shadows or impair image contrast or resolution Keep eyepieces always clean Leave the analyzer slider the polarizer slider and the reticle slider in their respective po...

Page 54: ...advance mode switch on the top panel of the dark box to A AUTO mode 1 When using NFX 35 dark box Set the mode switch on the bottom I panel of the dark box to B CD Pull the rewind knob to open the rear cover I CD Load a film cartridge into the dark box Push back the rewind knob 12l G Align the tip of film leader with the red film tip index mark of the dark box If the film is pulled longer than nece...

Page 55: ...x mark and affect correct film advance Initially advance the film Touch the WIND key of the control box once to automatically advance the film until the frame counter of the dark box indicates 1 This however is possible only when the dark box is connected to the microscope base with the DX cable If the DX cable is not connected to the dark box keep touching the WIND key until the film counter indi...

Page 56: ...the film is being rewinded The slight tension felt near the film end is the sign that the film is released from the spool Turn the crank a little further until the film advance indicator stops rotating Now the film is rewound completely FX 35DX NFX 35 G Pull the rewind knob to open the rear cover and unload the film cartridge Unload the film cartridg None e in a place with no direct sunlight Do no...

Page 57: ...el Exposure time is automatically determined through brightness measurement right before the shutter opens If necessary touch either of EXP ADJ exposure adjustment keys to adjust exposure time Touch EXP exposure key Then countdowndisplay of exposure time starts The shutter closes at 000 00 000 00 shows the end of countdown Set TIME or MULTIPLE exposure mode to cancel automatic exposure mode Note 1...

Page 58: ... Automatic advance of film after exposure is omitted Touch D EXP key to set multiple exposure mode D EXP 0 Identical as in the cases of automatic and TIME exposure Identical as in the cases of automatic and TIME exposure modes Touch RST key to cancel multiple exposure mode Notes 3 4 RST ote 4 Multiple exposure mode can be canceled in the following two ways to touch RST key before or after making t...

Page 59: ...pen or stop down down aperture and field diaphragms To J on page 54 Tum on the power switch of the control box See page 56 DX mode If the film loaded is with DX code its ISO value is automatically set Non DX mode Touch either of the ISO keys to set an arbitrary ISO value See page 56 See page 58 See page 58 See page 59 See page 60 on how to adjust these two diaphragms of the microscope ...

Page 60: ... 60 For the color films adjust the brightness with ND filters to set the exposure time within the specified range For B W films change lamp intensity to arbitrarily set the exposure time Touch the EXP key of the control box or depress the hand held switch See page 61 The shutter opens and closes Exposure The remaining exposure time is counted down Large format film Advance film To 35 mm film Film ...

Page 61: ...e measuring range Rotate the lamp intensity control dial of the microscope to change the lamp intensity Or use appropriate ND filters to adjust the brightness for color photomicrography The shutter is locked because of failure in operation or malfunction If this LED does not stop blinking when the RST key is touched contact your Nikon representative Touch the RST key Confirm whether or not the DX ...

Page 62: ... marks above the ISO keys show the directions in which the lighted LED moves f to the left to the right 1 J 0 1 00 00 Initial display of I fi 1 1 a I the exposure time Lighted LED rn c rn Ex P 40J 2 1 12 Ji lO 4 llO O l llilla ll D llllllmml2CDll t P OJ ISO W HO RST i 1s0 key D EXP ADJ keys The DX mode is the mode that automatically reads out and sets the ISO value from the DX code printed on the ...

Page 63: ...shift the LED in the direction to make the exposure time longer Touch the key below to shift it in the direction to make the exposure time shorter Ex Set the exposure adjustment at O when the sample image is uniformly distributed within the photomask When the target objects within the sample image are darker than the surrounding shift a lighted LED on the EXP ADJ value display in the direction for...

Page 64: ...s Daylight type color photomicrography Set the lamp intensity control dial at 9V and put NCB11 filter in the optical path Use appropriate ND filters to adjust the brightness The NCBJI filter is particularly suitedfor use with standard colorfilm Since the color reproduction characteristics ofeach colorfilm slightly differ depending on its make combine color compensation CC filters with the NCRfilte...

Page 65: ...al double crosshairs built in the eyepiece Bring the double crosshairs into focus With the crosshairs in focus slowly rotate the fine focus knob of the microscope to focus on the sample image Keep rotating the focus knob until both the sample image and crosshairs come into focus together Intermediate magnification objective lOx or 20x Focus on the double crosshairs and the sample image in the same...

Page 66: ...appropriate voltages The table below shows common combination of lamp voltages and the filters 9 Exposure Time The exposure time should be set between 1 4 and 1 15 of a second How to set exposure time Exposure time is displayed within the range of 0 01 to 999 99 seconds 1 100 of a second to approximately 16 minutes according to the measured brightness of the sample The display of the exposure time...

Page 67: ...tinue the exposure in progress in case of emergency or to reset the multiple exposure mode Also touch this key when the exposure time display fails to indicate 000 00 the moment the power switch is turned on or when an LED blinks to indicate E error on the CHECK display If 000 00 still fails to appear or if the LED indicating E does not go out contact your Nikon representative 12 Film Advance 35 m...

Page 68: ...ackground You may not need to put it in for a sample with dark background t Data mask Data mask is in the film frame Inside the rearcover of the dark box ________________________________ _ I When the optional data back is not used keep the IN OUT lever always turned to OUT to perform photomicrography without the data I I 1 mask 1 Refer to the instruction manual of your data back on how to operate ...

Page 69: ...not intermediate position position P 44 45 visible Field diaphragm stopped down more than Open it properly P 36 necessary Dirt or dust on lenses objectives or eyepieces Clean them or on specimen Revolving nosepiece not correctly mounted Remount it correctly P 6 Mirror block in intermediate position Remount it correctly P 8 Filters or lamp not correctly mounted Remount filters or lamp correctly P 5...

Page 70: ...ure diaphragm stopped down more than brightness necessary Optical changeover levers are not operated to provide 100 light output for eyepieces Light output is set to the side port or to the 35 mm large format Actions Fix specimen so that its surface totally attaches the stage surface Refit it to stage correctly Remount it correctly P 6 Rotate it to click stop Remount nosepiece to the limit and cla...

Page 71: ...sted Adjust eyepiece diopter switched to other correctly P 15 objectives Image movement not smooth when Top of objective in contact with stage ring Change sample and the sample is stage position moved Binocular images lnterpulillary distance not correctly adjusted Adjust it correctly not coincidnet P 17 Excessive eye Diopter difference not correctly adjusted Adjust it correctly P 15 fatigue during...

Page 72: ...ted to power supply doesn t function unit Lamp is about to blow Lamp flickering or Cables not firmly plugged into connectors unstable lamp intensity Lamp not inserted into socket firmly Actions Connect power cable to AC IN line receptacle Connect it to the power supply unit P 8 Mount one P 5 6 Replace the lamp P 5 6 Use specifield lamp See Electrical Specifications on page 72 Use specified lamp Se...

Page 73: ...d Do not use cover glass to the sample Grease dust dirt or Clean top of objective uppermost part of eyepiece finger prints on optical specimen projection lens or the like Pay Image blurs system uninterrupted attention to cleaning and custody of optical system When not in use put cover on microscope Adjustment of Readjust it correctly P 18 22 illumination system Immediately replace old blackend lam...

Page 74: ... 1000 times of objective s numerical aperture Photograph blurs if taken beyond this range Darken surrounding environment or cover eyepieces with caps Do not expose microscope or sample to hazardous strong light such as direct sunlight Use NCB11 filter P 56 Spectral sensitivity of daylight type film differs considerably from type to type or from manufacturer to manufacturer Varieties of film of ide...

Page 75: ...s Clean the contact points of the dark box and its attaching mount on the microscope with a clean soft cloth or a tissue paper lightly moistened with alcohol Connect the DX cable from the microscope base to the control box Connect the other DX cable from the microscope base to the dark box P 9 10 Set the switch properly P 10 Data mask in the dark Turn the data mask IN OUT lever to box partially in...

Page 76: ... inflammable Never use them near the fire Pay close attention to their handling when turning on or turning off power switches or when handling other similar devices to prevent absolute alcohol or benzine from catching fire 2 Cleaning the Large Format Mirror If dust on the large format mirror is imprinted on a photomicrograph clean the mirror as described below 1 Detach the focusing panel Push the ...

Page 77: ...er and the like to clean the painted surfaces plastic or printed parts of the microscope It is advisable to use a silicon cloth to clean these surfaces or parts 4 Custody of the Microscope Eyepieces and Objectives Keep the microscope in custody in a dry place where mold will not be formed easily Keep the objectives and eyepieces in a container such as a desiccator with a desiccant in it When keepi...

Page 78: ...for 100 120V Input Voltage Input ratings 100 120V 3A 50 60Hz Output ratings 12V DC 100W Protection class Class I Conforming standards This product conforms to UL 1262 Power supply cord Use only the following cord set instruction UL Listed detachable cord set 3 conductor grounding type SVT No 18 AWG rated a minimum 125V 7A PSM 2120 Power Supply Unit for 220 240V Input Voltage Input ratings 220 240V...

Page 79: ...CONTROL BOX Input ratings 100 120V 50 60Hz or 220 240V 50 60 Hz switchable Fuse 1A 250V Battery Ni Cd battery 3 6V 50 mAH Operating environment Ambient temperature 0 c to 40 C Relative humidity 85 max 73 ...

Page 80: ...ht to make such altera tions in design as may be considered necessary in the light of experience For this reason particulars and illustrations in this handbook may not conform in every detail to models in current production ...

Search results

Summary of Contents for Epiphot 300

Reviews:

Related manuals for Epiphot 300

Brands by name

Popular brands

Nikon Epiphot 300, Manual

Search results

Summary of Contents for Epiphot 300

Reviews:

Related manuals for Epiphot 300

Brands by name

Popular brands