Nikon ECLIPSE E200 MV Series Instruction Manual Download | Manualshive

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Chapter 5 Miscellaneous Operations

4 Eye Level Raiser

25

5

4

Eye Level Raiser

The eye level raiser (optional) adjusts the height of
the eyepiece tube according to the eye level of the
user. Install the eye level raiser between the main
body and the eyepiece tube. One eye level raiser is
25 mm high. You can use up to two raisers

−

this

raises the eyepiece tube by 50 mm. Use a
hexagonal wrench (nominal size 2) for M4 set
screws to install the raisers.

5

Photomicrography and TV Microscopy

The microscopes ECLIPSE E200 MV series and E200LED MV series can be used for
photomicrography and TV microscopy.
For those observations, trinocular eyepiece tube, photomicrographic equipment, TV vertical tube,
and C-mount CCTV camera (optional) are available. Read the manuals provided with these
devices for installation and operating conditions.
Eyepiece tubes and relay lenses come in various types. For details, contact your nearest Nikon
representative.

●

Trinocular Eyepiece Tube

Push or pull the optical-path selection lever
to adjust the amount of light sent to the
binocular part and the vertical tube.
The eyepiece tubes for the ECLIPSE Ci and
Ni series or 50i and 80i series can be also used.

●

Vertical Tube Adapters

When installing the photomicrographic equipment and TV camera on the vertical tube of the
trinocular eyepiece tube, vertical tube adapters are required in-between. For details, contact
your nearest Nikon representative. Typical combinations are shown below.

•

Photomicrographic equipment: Use the TV vertical tube and the photomicrographic vertical

tube

adapter.

•

C-mount CCTV camera:

Use the TV vertical tube and the direct C-mount adapter.

The relay magnification is 1x.

•

Digital still camera:

Use the TV vertical tube and the direct C-mount adapter.

Use the relay lens for the C-mount adapter, as necessary.

Eye level raiser

Distribution of light for

trinocular eyepiece tube (example)

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Summary of Contents for ECLIPSE E200 MV Series

Page 1: ...Microscope ECLIPSE E200 MV Series E200LED MV Series Instructions M588E 11 10 NC 1 1 2 ...

Page 2: ......

Page 3: ...made to ensure the accuracy of this manual errors or inconsistencies may remain If you note any points that are unclear or incorrect please contact your nearest Nikon representative Some of the equipment described in this manual may not be included in the set you have purchased If you intend to use any other equipment with this product read the manual for that equipment too If the equipment is use...

Page 4: ... Field diaphragm control is only for microscopes with the field diaphragm 9 4 Bring the 10x objective into the optical path 9 5 Place the specimen slide on the stage and bring the specimen into the optical path 10 6 Focus on the specimen 10 7 Adjust the diopter ring on the eyepieces 12 8 Adjust the interpupillary distance 13 9 Focus on the field diaphragm and center its position This adjustment is...

Page 5: ...nts 30 3 Replacement of Consumable Materials 32 Chapter 7 Optical Characteristics 34 1 Combinations of 10x Field No 20 Eyepiece with E Plan Objectives 34 2 Microscope Terminology 34 Chapter 8 Troubleshooting 36 1 Optical 36 2 Operational 41 3 Electrical 43 Chapter 9 Care and Maintenance 44 1 Cleaning the Lenses 44 2 Cleaning the Microscope 44 3 Disinfecting the Microscope 44 4 Storing the Microsco...

Page 6: ...perty damage To ensure correct usage read this manual carefully before using the product Do not discard this manual and keep it handy for easy reference Safety instructions in this manual are marked with the following symbols to highlight their importance For your safety always follow the instructions marked with these symbols Symbol Description WARNING Disregarding instructions marked with this s...

Page 7: ... 30 minutes after turning off the lamp and make sure that the lamp and its surrounding areas have cooled sufficiently before working When replacing the lamp see WARNING 5 Cautions on lamp replacement for E200 MV series only on P vi to follow what is described there CAUTION HOT for E200 MV series only This symbol can be found when the field lens unit is removed from the microscope body and cautions...

Page 8: ...approximately 30 minutes after turning off the lamp and make sure that the lamp and the field lens unit have cooled sufficiently To prevent electric shock and product damage turn off the power switch press to the position and unplug the power cord from the wall outlet before replacing the lamp When replacing the lamp be sure that the lamp socket is not damaged If it is damaged it may cause lamp fa...

Page 9: ...solute alcohol ethyl alcohol or methyl alcohol for cleaning optical parts Petroleum benzine for wiping off the immersion oil Medical alcohol for disinfecting the microscope Never hold a flame near these solvents When using a solvent thoroughly read the instructions provided by the manufacturer and handle correctly and safely Note the following precautions when using solvents with the product Keep ...

Page 10: ...ns If liquids or foreign matters enter the product do not use the product and contact your nearest Nikon representative 3 Cautions on moving the product When carrying the microscope hold the microscope firmly by the bottom front recess and the top rear When moving the product do not hold by the focusing knobs eyepiece tube and stage etc The parts may become detached and cause the product to fall a...

Page 11: ...al exposure to hazards in the event of earthquakes and other potential disasters If necessary secure the product to the working desk or other heavy and stable items with a strong rope or other means so as to prevent it from falling Install the product so that the power cord to the microscope can be unplugged immediately from the AC inlet in case of an emergency Avoid placing the product in direct ...

Page 12: ...tion device The knob turns freely for a while after it has reached its upper limit 8 Oil immersion observation Use only a minimum quantity of oil If too much oil is applied surplus oil may flow out to the stage and the condenser which may lead to degraded performance When using petroleum benzine or absolute alcohol to wipe off immersion oil or to clean the lenses follow the instructions provided b...

Page 13: ......

Page 14: ... The condenser should be positioned slightly lower than its upper limit Adjust the aperture diaphragm lever according to the objective 6 Field Lens Unit Draw out the field lens unit when changing lamp for E200 MV series only The microscope may have a field diaphragm A field diaphragm is used to control the illumination range and should be adjusted according to the objective Note that there are two...

Page 15: ...this figure is an example of ECLIPSE E200 MV RS The light source for E200 MV series is halogen lamp and the light source for E200LED MV series is white LED 2 Eyepieces 3 Eyepiece Tube 1 Main Body 4 Objectives 5 Condenser 6 Field Lens Unit 7 Lamp 8 Power Cord ...

Page 16: ...aphragm image P 14 These screws are equipped only on the microscope with a field diaphragm 13 Fine focus knob Used for fine focusing 14 Power switch When pressed to the l position power is turned on and the lamp lights When pressed to the position power is turned off and the lamp goes off 15 Brightness control dial When turned clockwise the lamp voltage increases and the viewfield becomes brighter...

Page 17: ...ondenser clamp screw 16 Condenser focus knob 19 Coarse focus knob torque adjustment ring 20 Nameplate 17 Fine focus knob 21 AC inlet 13 Fine focus knob 10 Lateral stage motion X Axis knob 9 Longitudinal stage motion Y Axis knob 15 Brightness control dial 14 Power switch 12 Field diaphragm centering screws 11 Field diaphragm ring 8 Filter holder 18 Coarse focus Knob ...

Page 18: ...ate the stage motion Y axis and X axis knobs to bring the specimen into the optical path 6 Focus on the specimen P10 Turn the focus knobs to focus on the specimen 7 Adjust the diopter P12 Turn the diopter ring on the eyepieces 8 Adjust the interpupillary distance Match the distance between the eyepieces with the distance between your eyes 9 Focus on the field diaphragm and center its position This...

Page 19: ...roscopes with the field diaphragm Turn the field diaphragm ring until the diaphragm image just circumscribes the viewfield 14 Perform the microscopy P18 Whenever the objective is changed adjust the condenser aperture and field aperture diaphragms 15 Turn off the power switch Press the power switch to the position ...

Page 20: ...rse focus knob torque adjustment ring are located on the opposite side of the stage motion knobs The fine focus knob is coaxial and located on both sides Preparation Operations previous to the microscopy 1 Turn on the power switch Press the power switch to the l position and the lamp will come on 2 Lower the condenser slightly from its upper limit Turn the condenser focus knob to raise the condens...

Page 21: ...hragm ring to the left limit to fully open the diaphragm 2 Turn the condenser aperture diaphragm lever to the left limit to fully open the diaphragm 4 Bring the 10x objective into the optical path Rotate the revolving nosepiece to bring the 10x objective into the optical path The objective will click into place when rotated into position 1 2 Field diaphragm ring Condenser aperture diaphragm lever ...

Page 22: ...e side without the stage motion knobs Do NOT turn the coarse focus knob further after the stage has reached its upper limit This operation will damage the focusing mechanism When you raise the stage using the coarse focus knob take your eyes off the eyepieces and look at the gap between the upper surface of the specimen and the front of the objective from the side Since the working distances of 10...

Page 23: ...stage When the specimen image appears stop rotating the knob Do NOT turn the coarse focus knob further after the stage has reached its lower limit This operation will damage the focusing mechanism 4 Rotate the fine focus knob and precisely focus on the image Do NOT turn the right and left focus knobs simultaneously in opposite directions This operation will damage the focusing mechanism 2 4 Coarse...

Page 24: ...in the optical path Rotate the coarse and then fine focus knobs to bring the specimen in focus Refer to step 6 for focusing 3 Switch back to the 10x or 4x objective 4 While looking into the right eyepiece with your right eye focus on the specimen by rotating the right diopter ring without using the focus knob 5 While looking into the left eyepiece with your left eye focus on the specimen by rotati...

Page 25: ...rtable in observing the image due to the lower eye level you may find this convenient Before storing the microscope in the cabinet put back the binocular eyepiece tube to its original lower position If not the tip of the eyepiece may hit against the shelf when storing or the microscope cannot be stored in the cabinet The eye level can be raised even higher till 50 mm if an Eye Level Raiser optiona...

Page 26: ...age focused with the 40x objective is not so clear as that focused with the 10x objective 5 Turn the field diaphragm ring so that the field diaphragm image may become about the same size as the viewfield 6 If the field diaphragm image is not centered use the centering screws again If the field diaphragm image is not in the exact center of the viewfield it will be off centered especially when stopp...

Page 27: ... 11 and 13 11Adjust the condenser aperture diaphragm Turn the condenser aperture diaphragm lever to the same figure as the magnification of objective in the optical path The figures on the condenser show the approximate positions of the aperture diaphragm lever corresponding to each objective magnification When the aperture diaphragm lever is moved to that position the size of the aperture diaphra...

Page 28: ...umination Do not use it to control brightness Use the brightness control dial to control the brightness The objective s numerical aperture is indicated on the side of the objective 40x 0 65 magnifying power 40x numerical aperture 0 65 The image of the aperture diaphragm cannot be seen through the eyepiece To observe the diaphragm image remove the eyepiece and look down the open tube The eyepiece i...

Page 29: ...en damage the specimen by pressing it against the objective Before breaking the coverglasses or damaging the objectives read the following and find the correct way to focus on the specimen 1 When rotating the coarse focus knob while looking into the eyepieces be sure to turn it only in the direction that lowers the stage 2 When raising the stage using the coarse focus knob take your eyes off the e...

Page 30: ...ions turn the field diaphragm ring until the diaphragm image just circumscribes the viewfield The figures around the field diaphragm show the approximate positions for field diaphragm ring corresponding to each objective magnification when 10x eyepieces are used For 100x objective the field diaphragm cannot be closed enough 14Perform the microscopy Rotate the stage motion knobs to move the specime...

Page 31: ...m for easy specimen replacement 1 Use one hand to gently press down the stage 2 2 While holding the stage at that position change the specimen 3 Gradually release the stage so that it rises slowly The stage will return to the focal position 1 The distance between the front of the objective and the specimen when the specimen is in focus is called the working distance of the objective For details se...

Page 32: ... objective becomes slightly smaller than the working distance take your hands off the coarse focus knob The specimen is now almost in focus Look into the eyepieces and rotate the fine focus knob in the direction that lowers the stage Method 2 Swing the 40x objective into the optical path While looking at the microscope from the side rotate the coarse focus knob until the specimen almost touches th...

Page 33: ...en storing the microscope Unplug the power cord Before storing the microscope wait approximately 30 minutes after turning off the lamp and make sure that the field lens unit has cooled sufficiently Return the binocular part to its lowest position Cover the microscope with the vinyl dust cover Before covering the microscope wait approximately 30 minutes after turning off the lamp and make sure that...

Page 34: ...ondenser from the long hole on the stage Bring the specimen back over the condenser and slowly raise the condenser Objective Rotate the revolving nosepiece to move the objective out of position Add a drop of oil to the specimen Slowly rotate the revolving nosepiece to bring the objective back into position Eliminate Air Bubbles Make sure that air bubbles are not trapped during oil application Air ...

Page 35: ... weaker than petroleum benzine When handling petroleum benzine and absolute alcohol be sure to follow the instructions provided by the manufacturers Since they are highly flammable take great care when handling them Cautions on Handling the Immersion Oil Close the container cap tightly after use Make sure that the cap is closed tight after refilling the container Check the cap periodically to make...

Page 36: ...with a small working distance is used Using the upper limit bolt the stage does not move up from a certain position Attach the bolt as follows 1 Bring an objective with 40x or larger power into the optical path Focus on the specimen 2 Lower the stage to slightly below the position set in step 1 3 Remove the field lens unit 4 A hexagonal socket head bolt is located in the left hole at the lower par...

Page 37: ...ubes and relay lenses come in various types For details contact your nearest Nikon representative Trinocular Eyepiece Tube Push or pull the optical path selection lever to adjust the amount of light sent to the binocular part and the vertical tube The eyepiece tubes for the ECLIPSE Ci and Ni series or 50i and 80i series can be also used Vertical Tube Adapters When installing the photomicrographic ...

Page 38: ...used for the photomicrographic equipment or digital still camera the viewfield may look partially dark Make the brightness of the viewfield uniform using one of the following methods Lower the condenser Slightly lower the condenser to take more space between the specimen and the condenser If the microscope is equipped with a field diaphragm the field diaphragm image may blur slightly Use a phase c...

Page 39: ...rs Push the cord hangers optional into the holes on the rear of the microscope The hangers can be used for winding the power cord around when the microscope is not in use To remove the hangers use a screwdriver E200 MV series E200LED MV series Cord hangers Cord hangers ...

Page 40: ...uct is a precision optical instrument Handle the product with care and avoid subjecting it to strong physical shocks In particular the accuracy of objectives may be lost by even weak physical shocks This chapter describes the installation assembly and setup of the product in the actual sequence Assemble the product as described in this chapter Tools Required for Assembly Hexagonal wrench one hexag...

Page 41: ...lts Remove the bolts and the plate 3 Installing the Eyepiece Tube Loosen the eyepiece tube clamp screw by hand and place the eyepiece tube on the circular dovetail mount Tighten up the eyepiece tube clamp screw by hand 4 Connecting the Power Cord Turn off the power switch of the microscope press to the position Connect one end socket of the supplied power cord to the AC inlet on the rear of the mi...

Page 42: ... bottom of the condenser 7 Focus the condenser so that light passing through the condenser focus the image on the correct position of the specimen center of the optical path z Microscopes without the field diaphragm Rotate the condenser focus knob to raise the condenser to its full limit and then slightly lower it to the position where the diffuser image can no longer be seen in the viewfield z Mi...

Page 43: ... furthermost from the specimen using the hexagonal wrench provided 4 Eyepiece and Eyepiece Pointer The 10x eyepieces are attached to the eyepiece tube before shipment When removing an eyepiece loosen the eyepiece set screws using the hexagonal wrench provided and then remove the eyepiece When attaching an eyepiece push the eyepiece down to its full limit and tighten up the set screw Be careful not...

Page 44: ...amaged they may cause poor illumination or overheating Insert the lamp s contact pins fully into the socket holes If the pins are loose the lamp may come loose or result in a contact failure which will cause overheating or smoke Be sure to put the field lens unit back in place after replacing the lamp Never turn on the lamp without the field lens unit Do not touch the glass part of the lamp using ...

Page 45: ...ts surroundings are cool enough to touch 3 Hold the field lens unit at the vertical grooves on both sides and pull it toward you to remove it 4 Remove the old lamp 5 Hold a new lamp wearing gloves or using a cloth Insert the lamp s contact pins fully into the socket holes straight and securely 6 Put the field lens unit back in place 7 Plug the power cord Field lens unit Socket Lamp ...

Page 46: ...m air immersion oil etc between the objective and the specimen or condenser and α is half of the maximum angle at which light enters or leaves the lens from or to a focused object point on the optical axis The larger the numerical aperture the brighter the image and the higher the resolution 3 Resolving Power The ability of an optical system to discriminate between two discrete objects separated b...

Page 47: ...Depth of Focus The depth thickness of the specimen image in focus extending above and below the focused image plane The larger the N A of the objective the shallower the depth of focus Depth of focus μm 2 2 n Α Ν λ M n Α Ν 7 1000 Above is an approximation assuming the resolving power of an eye as 2 minutes λ is the wavelength of light used The depth of focus in the table on P 34 is indicated forλ ...

Page 48: ...in and out of view when the condenser is moved up and down while using low magnification objective Clean the condenser P 44 Objective is dirty if dirt or dust disappears when the objective is switched Clean the objective P 44 The field lens unit is dirty if dirt or dust moves when field lens unit is moved slightly Clean the field lens unit P 44 The eyepiece is dirty if dirt or dust does not move w...

Page 49: ...not correct If the microscope is equipped with a field diaphragm correct positioning so that the field diaphragm image is focused on the specimen surface P 14 If the microscope is not equipped with a field diaphragm position the condenser slightly lower than the upper limit No immersion oil is applied to the tip of the oil immersion objective Apply Nikon immersion oil to the objective P 22 Nikon i...

Page 50: ...with a field diaphragm correct positioning so that the field diaphragm image is focused on the specimen surface P 14 If the microscope is not equipped with a field diaphragm position the condenser slightly lower than the upper limit When an optical path switchable eyepiece tube is attached optical path is not switched to the eyepiece 100 Switch the optical path to the eyepiece 100 The lamp has rea...

Page 51: ...t installed correctly Install correctly P 30 Field diaphragm is not centered if the microscope is equipped with a field diaphragm Center the field diaphragm P 14 Field diaphragm is closed too far if the microscope is equipped with a field diaphragm Open properly P 18 Field lens unit is not installed correctly Install correctly P 33 Lamp is not installed correctly for E200 MV series only Install co...

Page 52: ...ecimen rises from stage surface Stabilize it using the holder Image shifts during focus Becomes asymmetrically defocused when moving the focal point Causes Corrective Measures Revolving nosepiece is not in click stop position Revolve to click stop position Specimen rises from stage surface Stabilize it using the holder Field diaphragm is not centered if the microscope is equipped with a field diap...

Page 53: ...Tip of the objectives without stopper can not be rotated Do not use force to draw the stopper Contact your nearest Nikon representative High magnification objective contacts slide when changed over from low magnification Causes Corrective Measures Slide is upside down Turn over the slide so that the cover glass faces up Cover glass is too thick Use a cover glass of the specified thickness 0 17 mm ...

Page 54: ...rectly Adjust P 12 Excessive eye fatigue Causes Corrective Measures Interpupillary distance is not adjusted correctly Adjust P 13 Diopter is not adjusted correctly Adjust P 12 Inadequate brightness or illumination Adjust brightness using the control dial P 17 Specimen does not move smoothly Causes Corrective Measures Specimen holder is not securely fastened to the stage Fasten securely P 31 ...

Page 55: ...correctly P 32 Lamp is burnt out for E200 MV series only Replace lamp P 32 Incorrect lamp is used for E200 MV series only Use the specified lamp P 32 Flickering or unstable lamp brightness Causes Corrective Measures Lamp is about to burn out for E200 MV series only Replace lamp P 32 Power cord is not correctly connected Connect correctly Lamp is not correctly inserted into socket for E200 MV serie...

Page 56: ...th neutral detergent and wipe lightly Using organic solvent may result in discoloration of the plastic parts 3 Disinfecting the Microscope We recommend that you use 70 medical alcohol for normal disinfection of the microscope In case of spillage of a sample to the microscope determine whether the sample is hazardous If the sample is hazardous follow the standard procedure of your laboratory Using ...

Page 57: ...rofessional and those who work on experimentations and practitioners of the microscope observation at educational institutes in the field of medicine anatomy and cellular biology 2 Performance Properties 1 Model Name Microscope Main Body E200 MV Series ECLIPSE E200 MV L Stage knob left side without field diaphragm ECLIPSE E200 MV LS Stage knob left side with field diaphragm ECLIPSE E200 MV R Stage...

Page 58: ...0 to 240 VAC 50 60 Hz 0 1 A or less 8 Power Cord When used in 100 120 V region outside Japan UL listed detachable power cord set 3 conductor grounding 3 conductor grounding Type SVT No 18 AWG 3 m long maximum rated at 125 V AC minimum When used in 220 240 V region Detachable power cord set approved according to EU EN standard 3 conductor grounding 3 conductor grounding Type H05VV F 3 m long maximu...

Page 59: ... side with field diaphragm ECLIPSE E200LED MV R Stage knob right side without field diaphragm ECLIPSE E200LED MV RS Stage knob right side with field diaphragm Eyepiece Tube E2 TB Binocular eyepiece tube E2 TF Trinocular eyepiece tube 2 Dimensions and Weight 227 W 382 D 415 H mm 8 Kg Combination with Microscope Main Body E200 MV L Eyepiece Tube E2 TB 3 Operating Environment Room Temperature 0 to 40...

Page 60: ... in accordance with the instruction manual may cause harmful interference to radio communications Operation of this equipment in a residential area is likely to cause harmful interference in which case the user will be required to correct the interference at his own expense This product complies with Canadian EMI ICES 003 Class A This Class A digital apparatus complies with Canadian ICES 003 Cet a...

Page 61: ... 3 2 2 3 1 4 4 1 0 4 4 5 5 6 5 0 7 Jp 䝬䞀䝱䝇䝕䛱䛐䛗䜑ᗣᲘ ืᅂ 䛴䜻䝷䝠䝯䝢䞀䜳䛙䛴䜻䝷䝠䝯䝢䞀䜳䛵ᮇ ဗ䛒 ื䛱ᅂ 䛛䜒䛰䛗䜒䛶䛰䜏䛰䛊䛙䛮䜘䛝䛬䛊䜄䛟䚯 ḗ㡧䛵ᮇ ဗ䜘䝬䞀䝱䝇䝕䟺㻨㻸䟻䛭䛟䜑ሔྙ䛱䛴䜅㐲䛛䜒䜄䛟䚯㻃 ᮇ ဗ䛵ᣞᏽ䛛䜒䛥㞗ሔᡜ䛭 ื䛱ᅂ 䛛䜒䜑䜎䛌䛱ᏽ䜇䜏䜒䛬䛊䜄䛟䚯 ᐓᗖ䜸䝣䛮䛝䛬ᗣᲘ䛝䛰䛊䛭䛕䛦䛛䛊䚯㻃リ䛱䛪䛊䛬䛵 ᗉ䜄䛥䛵ᆀᇡ䛴ᗣᲘ ฌ ᶭ㛭䛱䛚䛕䛦䛛䛊䚯 This symbol is provided for use in the People s Republic of China for environmental protection in the ﬁelds of electronic information products 䛙䛴䝢䞀䜳䛵䚮 ୯ᅗ䛴䛐ᐂᵕ䛱ྡྷ䛗䛥䜈䛴䛭䚮㞹Ꮔ ሒ ဗฦ...

Page 62: ...Amstelveen The Netherlands Laan van Kronenburg 2 1183 AS Amstelveen The Netherlands tel 31 20 44 96 300 tel 31 20 44 96 300 NIKON INSTRUMENTS SHANGHAI CO LTD NIKON INSTRUMENTS SHANGHAI CO LTD tel 86 21 6841 2050 tel 86 21 6841 2050 NIKON SINGAPORE PTE LTD NIKON SINGAPORE PTE LTD tel 65 6559 3618 tel 65 6559 3618 NIKON MALAYSIA SDN BHD NIKON MALAYSIA SDN BHD tel 60 3 7809 3688 tel 60 3 7809 3688 NI...

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