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VI Basic Operations 

 

MICROSCOPE 

 

 

6    Using the Microscope Control 

- 127 - 

• 

Control the lamp 

 

 

<Microscope control menu 2> 

 

• 

Change the condenser 

• 

Adjust the diascopic illumination aperture diaphragm 

• 

Adjust the diascopic illumination field diaphragm 

• 

Adjust the episcopic illumination field diaphragm 

• 

Adjust the transmission factor of the ND filter 

• 

Change the analyzer 

 

 

<Microscope status display/microscopy setup menu> (MIC Info menu) 

 

• 

Show the name of the objective 

• 

Show the position of the stage in height direction 

• 

Show the magnification of the optical zoom 

• 

Show the name of the filter 

• 

Set up microscopy and show the current microscopy 

 

 

<Precautions regarding menu display> 

 

Depending on the type of microscope connected to your system, not all items of the microscope 
control menus and microscope status display menus can be displayed. The items that can be 
displayed on the DS-L1 menu are limited to those for parts whose status can be detected 
electrically among the various parts of your microscope. Keep in mind that the items displayed 
on the DS-L1 menu do not include those for parts whose status cannot be detected electrically. 
Refer to the table below for the relationship between the microscope system and menu display. 

 

1) 

When the Nikon 80i microscope incorporating DIH-M is connected 

Parts Menu 

displayed 

Focus (Z axis) 

No menu displayed 

Intelligent nosepiece 

Status display menu displayed 

Nosepiece 

Manual nosepiece 

No menu displayed 

Optical zoom 

Status display menu displayed 

Optical path 

Status display menu displayed 

Shutter 

Control/status display menu displayed 

Filter cube 

Status display menu displayed 

Lamp 

No menu displayed 

Universal condenser 

No menu displayed 

Diascopic illumination aperture diaphragm 

No menu displayed 

Diascopic illumination field diaphragm 

No menu displayed 

Episcopic illumination field diaphragm 

No menu displayed 

ND filter 

No menu displayed 

Analyzer 

No menu displayed 

Summary of Contents for DS-5M

Page 1: ...DS Camera Control Unit DS L1 DS Camera Head DS 5M DS Cooled Camera Head DS 5Mc Instructions Ver 2 2 M306E 04 10 NF 6 1 2 ...

Page 2: ......

Page 3: ...use or failure to observe the instructions may result in injury or property damage To ensure correct use read this manual carefully before using your products Do not discard this manual Always keep it at hand for easy reference Safety instructions in this manual are indicated by the symbols shown below To ensure safety please follow the instructions indicated by these symbols Symbol Meaning Disreg...

Page 4: ...ment falls under the category of electric shock protection class I always make sure it is grounded to a protective ground terminal If you misplace or damage the power cord contact your nearest Nikon representative When using the product in 100 120 V AC regions Use an UL listed detachable power cord set 3 conductor grounding Type SVT No 18 AWG 3m long maximum rated at 125V AC minimum When using the...

Page 5: ...ent or when connecting disconnecting cables To prevent damage or malfunction always turn off the DS L1 s POWER switch when setting up the equipment or when connecting or disconnecting cables 3 Connect only the devices specified to the connectors To prevent damage to the equipment connect only the devices specified to the connectors on the DS L1 the DS Camera Head DS 5M DS 5Mc or the DS Remote Cont...

Page 6: ...1 Menu Configuration 24 1 3 2 Method of Displaying a Menu 25 1 4 Initial Settings 26 1 4 1 Date and Power save Settings Additional Settings 26 1 4 2 Image File Settings File Settings 28 1 4 3 Startup Condition Settings Power On Settings 32 1 4 4 Adjusting Monitor Output for an External Monitor 33 1 4 5 Settings for Direct Print 34 1 4 6 Settings for Connecting a Microscope 35 2 Photographing Image...

Page 7: ...Image 78 4 Using the DS L1 via a Network 79 4 1 Typical Method of Use 79 4 2 Network Settings 81 4 2 1 Items Needing Confirmation 81 4 2 2 Setting Essential Items Network Settings 82 4 3 Operating the DS L1 from a WEB Browser 85 4 3 1 Setting the WEB Browser 85 4 3 1 1 When Using Internet Explorer 85 4 3 1 2 When Using Netscape 87 4 3 2 Using WEB Screens 89 4 4 Using FTP Commands to Operate the DS...

Page 8: ...w 126 6 2 Menu Overview 126 6 2 1 Microscope Control Menu 1 131 6 2 2 Microscope Control Menu 2 137 6 2 3 Microscope Status Display Microscopy Setup Menu MIC Info Menu 140 6 3 Autofocus Precautions 144 VII Care and Maintenance 147 1 Cleaning the System 147 2 Storage 147 VIII Troubleshooting 148 1 Power Supply 148 2 Image Output 149 3 Photographing and Saving 151 4 Abnormal Behavior 151 5 Networkin...

Page 9: ...ontact your nearest Nikon representative immediately 1 DS Camera Control Unit DS L1 5 DS Camera Head DS 5M or DS Cooled Camera Head DS 5Mc C mount cap Camera cable 3 m E229586 2 Instruction manual 6 DS Remote Controller DS RC Remote cable E120411 3 DS AC adapter 7 Direct Print License optional 4 Power cord 8 C mount adapter optional 9 Microscope Control License optional ...

Page 10: ... until the DS L1 has started up This forcibly sets the DS L1 to internal monitor output and external XGA output modes when it starts up After the DS L1 has started up set to XGA in Power On Settings of the SETUP menu and save your settings NOTE If you are using an LCD display or LCD projector we recommend adjusting its clock phase and clock pitch 2 USB mouse You ll need a USB mouse to access funct...

Page 11: ...ompliant CF card or CF Type II microdrive 1GB 7 C mount lens A C mount lens is required to photograph subjects other than photomicrographs Any lens that satisfies the following conditions may be used Protrudes no more than 8 mm from the mount surface into the camera The image circle is 2 3 Use a lens for 2 3 or larger e g 1 When using diaphragm settings brighter than F2 8 you may observe slight li...

Page 12: ... 7 0 Although Nikon has confirmed that the DS L1 operates properly under the above conditions DS L1 operations cannot always be guaranteed since they are affected by OS or browser versions and by various software settings 11 Nikon 80i 90i Microscope If you have a Nikon 80i 90i microscopes equipped with a digital imaging head you can purchase a license to control the microscope or display its statu...

Page 13: ...p a menu After a menu is displayed the MENU button can be used to confirm a selected menu command just like the ENTER button POWER switch This is a push switch Press it to turn on the power Press again to release it and turn off the power When the power is on the LED indicator lights and remains on ZOOM Electronic zoom button FREEZE Freeze pause button The freeze LED lights when the display is fro...

Page 14: ...keyboard USB printer CP900D or Nikon 80i 90i microscope Network Tx indicator Lights when sending data to a LAN Network Rx indicator Lights when receiving data from a LAN LAN port Connect the DS L1 to a LAN with a LAN cable to exchange data with a PC or server through a LAN 12 V DC output This output is currently unused 12 V DC input Use a dedicated AC adapter Stand arm Extract the arm to use it CF...

Page 15: ... details on the interface specifications External interface input Connect the DS Remote Controller DS RC here and you can perform freeze and capture operations from the DS RC A foot switch etc may also be used providing it meets the interface specifications Refer to 3 Connection Methods in Chapter IV for details on the interface specifications Camera connector Connect the DS Camera Head DS 5M or t...

Page 16: ...printer can be assigned Above functions can be assigned to the button in Additional Settings of the SETUP menu FREEZE button Freezes the current image as a still picture To release the frozen image press the button again C mount cap Protects the C mount from dust C mount When taking microphotographs mount the C mount adapter here before connecting to the microscope When taking other photographs at...

Page 17: ...S 5Mc CF card DS L1 DS AC adapter USB cable USB equipment mouse keyboard printer and Nikon 80i 90i misroscope LAN cable DS RC IV Connecting and Installing the Equipment 1 Connection Diagrams Schematic diagram PC or a PictBridge supporting printer DS camera cable LAN RGB cable ...

Page 18: ...n Diagrams 10 Connectors DS L1 Left side DS L1 Rear RGB cable External monitor Remote cable DS RC Remote cable DS 5M DS 5Mc USB cable USB equipment LAN cable PC or a PictBridge supporting printer DS AC adapter USB mouse USB keyboard Printer Microscope LAN ...

Page 19: ...vent heat from collecting near the equipment When attaching a commercially available arm to the DS L1 Any commercially available VESA standard compliant 75 mm arm UL1678 suited stand and mount can be attached to the DS L1 When doing so make sure the following specifications are met DS L1 weight 1 3 kg Fitting screw hole M4 7 mm deep Tightening torque 80 120 N cm Leave a clearance of about 100 mm a...

Page 20: ...4 Insert the C mount adapter into the vertical tube of the microscope Use the clamping screw on the vertical tube to secure the C mount adapter in place You can also attach a x0 7 relay lens When you have mounted the camera head on a microscope be sure to read Chapter V Microscope Adjustment as well Installing a third party C mount lens on the camera head Select a mountable lens see Chapter II Per...

Page 21: ... USB D connector on the DS L1 When connecting to a PictBridge supporting printer connect the PictBridge supporting printer and the USB D connector on DS L1 with a USB cable AB type 5 Connecting the DS Remote Controller DS RC Be sure to turn off the power for the DS L1 before making any connections Connect one end of the remote cable supplied with the unit to the EXTERNAL IN connector on the DS L1 ...

Page 22: ...e obtained when capturing an image If you are preparing your own external device use a device that satisfies the specifications shown below Connector 3 5 mm diameter stereo pin jack EXTERNAL IN connector Connector 2 5 mm diameter stereo pin jack EXTERNAL OUT connector EXTERNAL IN Functions Pin 1 FREEZE Pin 2 CAPTURE You will not be able to capture desired images at precise split second moments Use...

Page 23: ...gging a stroboscope or other external device into the EXTERNAL OUT connector of the DS L1 make sure the power for the external device is turned off If the power for the external device remains on your eyes may be damaged by unintended light emission 8 Connecting to a Power Supply To prevent electric shock turn off the power for the DS L1 before making any connections Complete all other connections...

Page 24: ...lor compensation filter CC filter can be inserted into the illumination optical path of the microscope to compensate for changes in color balance caused by the length of the exposure time When using a phase contrast microscope or an interference microscope two luminous fluxes or multiple luminous fluxes you can enhance contrast using a green interference filter GIF or a monochrome interference fil...

Page 25: ...se down the aperture diaphragm Note that closing down the aperture may limit the performance of the objective Terms What is depth of focus Depth of focus refers to the range along the axis of the field of view over which the image is in apparent focus Although reducing the aperture diaphragm extends the depth of focus doing so will also reduce resolution Take this into consideration and adjust the...

Page 26: ...ing a dark specimen with a fluorescent microscope for example ambient indoor light may enter the optical path Accordingly interior lighting should be darkened for photomicrography Place caps on the binocular eyepiece to prevent light from entering through the eyepiece 8 Effects of Vibration Because photomicrography involves extremely high image resolution the slightest vibration can affect image q...

Page 27: ...aphing 1 1 Using Various Input Devices 1 1 1 Using the DS L1 Panel Switches MENU button menu enter button Pressing the MENU button brings up the CAM Easy menu While the menu is displayed use this button as the ENTER button to confirm your selection MENU button Cursor buttons ZOOM button FREEZE button CAPTURE button ...

Page 28: ...xposure method on the CAM menu Select an icon you want and use the right or top cursor button to select items in the forward direction or the left or bottom cursor button to select items in the reverse direction ZOOM button electronic zoom button Use this button to apply an electronic zoom The image zooms in or out at the magnification selected for E Zoom Step in Additional Settings of the SETUP m...

Page 29: ...lue while right clicking the icon reduces the value For a shutter speed Left clicking reduces the shutter speed to increase exposure right clicking increases the shutter speed to reduce exposure For a mode select icon such as one to choose an exposure method on the CAM menu Left clicking lets you select items in the forward direction right clicking lets you select items in the reverse direction So...

Page 30: ... F1 CAM menu F2 VIEW menu F3 REC menu F4 TOOL menu F5 SETUP menu F6 MIC menu F7 F8 F9 E ZOOM F10 E ZOOM F11 FREEZE F12 CAPTURE Back Space Deletes one character during character entry SHIFT Backspace Deletes the entire text during character entry NOTE The DS L1 is factory set to a Japanese keyboard layout If you wish to use an English keyboard issue the Telnet command shown below to change keyboard...

Page 31: ...are formatted in FAT16 For detailed information on using the formatting function refer to 2 2 2 Reproducing Images from a CF Card VIEW Menu in Chapter VI NOTE CF cards formatted in other than NTFS may not be correctly formatted in FAT16 depending on the formats used In such cases reformat those CF cards in FAT12 FAT16 or FAT32 on your computer before use Insert the connecting side with the connect...

Page 32: ...s MIC Microscope control menu SETUP Power ON Settings Network Settings File Settings Additional Settings Startup settings Network settings File settings Other environment settings Return to window to return to the upper layer Shortcut Buttons for frequently used functions Freeze Electronic zoom AE lock Print Save to FTP server Save to CF card MIC INFO Settings of the frequently used status display...

Page 33: ...NU button once again Using the mouse Right clicking with the mouse displays a menu The specific menu displayed depends on the mouse pointer position on the screen when you right click Top 1 3th of the screen CAM Easy menu Middle of the screen CAM Easy menu and shortcut menu Bottom 1 3th of the screen Shortcut menu To close the menu screen right click anywhere outside the menu area Using the keyboa...

Page 34: ... for saving a log file at the same time 3 Startup condition settings Set startup conditions for photography and display states To make these settings display a menu and select the SETUP tag to enter the SETUP menu The correct procedures are detailed below 1 4 1 Date and Power save Settings Additional Settings Display a menu and select the SETUP tag to enter the SETUP menu Select Additional from th...

Page 35: ...ect the speed at which the mouse pointer moves across the screen when you move the mouse E Zoom Step Select the magnification rate of the electronic zoom at which rate the image is zoomed in or out Two or more choices can be selected Capture Function Save to Card Save to Server Print Select one of these functions to save to a CF card to save to the FTP server or to output to a printer you want ass...

Page 36: ...racters representing the date Example If the specified folder name in Card Dir is IMGBOX with the Auto check box selected and the date is 2003 05 05 then a folder named IM030505 will be created File Name Specify up to four leading characters for the name of the file in which you want to save an image A photographed image is saved under a file name comprising the specified file name and an automati...

Page 37: ...ge File Type Set a file format an image size and a compression rate TYPE Select JPEG or BMP as the format in which you want to save a file SOURCE Select s5M or s1 3M as the source photographing mode The source selected here is reflected in the image when you save it in a file or output directly to a printer Refer to Outline Guide to Source Photographing Mode Settings for an overview of how to make...

Page 38: ... Scan the source is automatically changed to s5M when you photograph an image NOTE You also can specify the source when saving an image from the REC menu List of performance levels achieved by a selected combination of sources and display modes Source Display mode CAM menu Performance 5Mi HR Lsp3 8 Rsp 1 3Mi HR Hsl Lsp6 7 1 3Mp HR Lsp7 5 MvL s5M C Scan HR Lsp15 MvL 5Mi Automatically changed to s5M...

Page 39: ...is enables observation at high speed for bright subjects or with high resolution for dark subjects without having to manually change display modes More specifically display modes are changed in the manner shown below For display mode 1 3Mp when the shutter speed falls below the 5Mi mode live speed the display mode changes to 5Mi Conversely if the shutter speed increases above the 1 3Mp mode live s...

Page 40: ...he custom mode to be used at startup NOTE The contents of the custom mode can be set from the CAM menu Cam OSD Ind Bio Select the type of scene mode button to be displayed in the CAM Easy menu Cross Line Selects whether to display or hide crosshairs when the power is turned on Refer to 3 5 1 Using Crosshairs to Adjust the Subject Position in Chapter VI Display Custom No 1 7 Seven types of screen d...

Page 41: ...1 is connected to an external monitor you must adjust the monitor output on the Power On Settings screen Monitor output is initially set to 1 024 768 resolution 60 Hz XGA If observation with a higher definition display is required when photographing an image we recommend using 1 280 1 024 resolution 60 Hz SXGA output NOTE If you select SXGA output images cannot be displayed on the DS L1 s internal...

Page 42: ...me to warm up sufficiently in accordance with the instruction manual 3 Display a menu and select the SETUP tag on it to enter the SETUP menu Select Additional from the SETUP menu to go on to the Additional Settings screen 4 Press the LCD Chart at the lower right corner of the screen A 1 dot pitched monochrome mesh chart will be displayed 5 Adjust the clock phase and clock pitch on the display side...

Page 43: ...pporting printer Or as another method connect a PictBridge supporting printer to the USB D connector on DS L1 first then enter a check mark in the PictBridge check box and turn the power for the PictBridge supporting printer on This also makes printing by the PictBridge supporting printer possible When this mode is selected the function to capture the image on the PC by connecting the PC to the US...

Page 44: ...an adjust the color and contrast 2 1 1 Simple Photography Easy Menu Select the EASY tab on the CAM menu and the Easy menu will be displayed The Easy menu allows you to photograph an image easily by only selecting the mode that suits the subject Configuration of the Easy menu Biological specimen scene mode Industrial specimen scene mode NOTE focus button and exposure start button are displayed only...

Page 45: ...ethod of observation 2 Adjust the white balance Prepare an evenly white subject and adjust the white balance with WB 3 Adjust the focus Adjust the image composition and the focus 4 Correct exposure Adjust the brightness of the image with CMP 00 5 Confirm the image Press freeze button to confirm the image During DF FL mode always use the exposure start button 6 Save the image Save the image with Ca...

Page 46: ... parts are expressed as transparent This mode can also be used for ceramic and plastic specimens Circuit Board This mode is suitable for photographing mounting defects on a part mounted circuit board Clipping of highlights in component leads and solder joints are suppressed allowing for defects to be easily detected This mode is also suitable for high contrast subjects such as gears and other meta...

Page 47: ...we recommend that the white balance be adjusted under normal light conditions before photographing an image If the screen is excessively dark or if clipping occurs adjust the luminous energy of the light source or the iris diaphragm or use an ND filter to obtain the appropriate white balance NOTE For especially dark subjects noise may become conspicuous 3 Adjusting the focus Determine an image com...

Page 48: ...r 4 6 3 Saving Image Files in the FTP Server in Chapter VI for details on how to use the REC menu 2 1 2 Special Photography on the Easy Menu DF FL Scene Mode In DF FL scene mode the camera is operated differently from other scene modes Press the DF FL scene mode button and you will enter high sensitivity display mode for focus adjustment At the same time focus button and exposure start button are ...

Page 49: ...servation method refer to 6 2 3 Microscope Status Display Microscopy Setup Menu MIC Info Menu in Chapter VI With this function the shutter opens by entering the DF FL scene mode in case the shutter has been closed Then when the exposure by the exposure start button is completed the shutter closes When performing the focusing again with the focus button or performing the exposure again by the expos...

Page 50: ...selected Lev Exposure level indicator Scene mode call button Focus Focus indicator 1 3Mp 1 3Mi 5Mi C Scan Display mode PROG S_AE MANU F_AE Exposure mode 1 250s Shutter speed C_gn 100 Camera sensitivity camera gain Photometry area Photometry mode CMP 00 Exposure compensation WB White balance AE_L AE lock Gain Y100 Image level adjust R100 B100 Color balance adjust CHRM 00 Color density HUE 00 Hue ad...

Page 51: ... procedure below find a method that is most suitable for the purpose of observation or the intended subject as you photograph an image 1 Choose display mode 2 Choose exposure mode 3 Choose photometry mode 4 Choose tone 5 Adjust the white balance 6 Adjust the picture quality 7 Confirm exposure information 8 Save the image 9 Save call photographing conditions ...

Page 52: ...i Ex Fluorescent photography nighttime photography with a lens Photographing a bright subject with priority placed on focusing C Scan Features of display modes The following describes features of each display mode 5Mi Observation with high definition images An image consisting of 5 07 million pixels is compressed for display at a rate of 3 75 frames per second Misregistration of colors may occur f...

Page 53: ...ode Choose your desired exposure mode from PROG S_AE MANU or F_AE Normally PROG should be selected Sample settings for different uses are described below Try these settings before determining your own settings Photographing a moving subject S_AE combination with 1 3Mp display mode Eliminating fluctuations of exposure for moving subjects or when moving a subject while observing MANU or use AE_L in ...

Page 54: ...DS 5Mc The camera sensitivity can be set from x1 C_gn 100 to x46 C_gn 4600 Effective for fluorescent photography where careful consideration is required to determine the exposure as well as for subjects that have partial highlight reflections Recommended example Fluorescent photography stereoscopic microscope observation metal photomicrography F_AE Choose F_AE when the subject is dark image update...

Page 55: ...riphery of the screen choose a narrow photometry range NOTE Displaying phoytometry area The photometry range is displayed with a red frame for a limited time when the ran right click again 4 Choosing tone When you set the tone the contrast will change Sample settings are shown below Confirm tones on the monitor when you set a tone and select the most suitable one To observe gradation in highlight ...

Page 56: ...one when you want to suppress unevenness in bright parts while photographing a high contrast image Recommended example Metal tissue or ceramics and other materials TONE7 Enhanced contrast Use this tone when you want to enhance the contrast during observation Recommended example Metal tissue or ceramics and other materials photographing documents with a lens etc 5 Adjusting the white balance Before...

Page 57: ... or tails on moving objects may occur when NR ON is selected When photographing a moving subject or if an increased response during focusing is desired select NR OFF Black level SFT 00 If you want to eliminate flare reflection in blacks on the screen or make blacks on the screen more distinct adjust the black level to the negative side Image level Gain Y100 Use this facility when fine adjustment i...

Page 58: ... will see 1 2 Save displayed on the screen Select the number in which you want to save your settings and press Save to save Note that shading can be corrected up to about 50 If the shading correctable range is exceeded an SD Data Error message will be displayed To correct shading with the saved data choose the shading correction icon SD US1 or SD US2 from the Advn menu 7 Confirming exposure inform...

Page 59: ...ted Conversely if the brightness distribution concentrates on the left edge of the histogram it means that the low lighted parts of the screen are blacked out Although it is ideal if no parts on the screen are saturated or blacked out exposure may have to be adjusted depending on the subject If an area of interest on the screen is highlighted adjust the exposure so that the distribution will be sh...

Page 60: ...ation in the Info box In the Advn menu you can save the set values of individual subjects and objectives in each custom mode 1 From the Advn menu set conditions such as camera operation mode and exposure NOTE Press Clset to temporarily clear the set values revert to the initial values set when shipped from the factory 2 Select the REG tab to enter the Registration menu 3 Select the custom mode num...

Page 61: ...nd camera gain manually fine adjustment of the displayed image can be performed 6 Press exposure start button and the DS L1 starts automatic exposure When finished a screen is displayed with a frozen image is displayed when exposure time is longer than 6 seconds Right click aborts the exposure after which image is freeze framed 7 If necessary adjust the shutter speed and camera gain manually and p...

Page 62: ...zed when the shutter speed of the DS L1 is in the following range 5Mi mode 1 3 s to 1 s 1 3Mp and 1 3Mi modes 1 7 s to 1 s Select MANU for exposure mode and set the shutter speed within the above range No noise reduction functions are supported Select NR OFF and leave it selected while in use The DS L1 does not have an automatic exposure function for stroboscope Use the automatical brightness adju...

Page 63: ... DS RC to save images to a CF card Before you can do this you must first select Save to Card for Capture Function in Additional Settings of the SETUP menu NOTE Refer to 2 1 1 Simple Photography Easy Menu in Chapter VI for details on how to use the CAPTURE button to save images The following describes how to save images from the REC menu Configuration of the REC menu NOTE Refer to 4 6 Saving Images...

Page 64: ... 480 900KB 450KB 220KB 150KB 120KB In the REC menu you can further save a comment or overlay a print along with the image Comment Select the Attach Log File check box and enter a comment in the input box That way you can save photographing information or comments in text format up to 100 characters at the same time you save an image You may enter the subject name number and the objective magnifica...

Page 65: ...in File Settings so that files will be named after the photographed date and time 1 Press 1shot to change to Continue The Cnt and Int icons will be displayed 2 In Cnt set the number of frames to be photographed Up to 500 frames can be set If Endless is selected in Cnt as the number of frames an image will be photographed successively 3 In Int set an interval time ranging from 10 seconds to 6 hours...

Page 66: ...se V or U to scroll the display up or down To use the panel switch or keyboard to scroll move the cursor to V or U and press the Enter button or key Erasing images Locate the file name you want to erase at the Z position and press erase button The image will be erased Displaying a thumbnail Locate the folder name whose thumbnail you want to display at the Z position and press thumbnail display but...

Page 67: ...NTFS i e formats not supported by the DS L1 the following message will be displayed on the VIEW menu Error Unacceptable CF Card Format FAT16 Format Press the Format button to format the CF card in FAT16 format NOTE CF cards formatted in other than NTFS may not be correctly formatted in FAT16 depending on the formats used ...

Page 68: ... mode by default In this mode an image is enlarged or reduced as will be suitable for the printer s paper size before being printed Real 10 print mode If the Mag Fix check box in Additional Settings is selected images are printed in real 10 mode When printing in this mode always be sure to use the x0 7 relay lens optional The scaling factor in this mode is equal to 10 times the objective magnifica...

Page 69: ...the Append check box for Log on the REC menu and enter a comment in the input box The comment will appear on the image being printed Comments can be up to 32 characters It is convenient to record the subject name number and the objective magnification for confirmation at a later time NOTE You can use the Telnet command to select whether to print information at the bottom of the printed image Do no...

Page 70: ...ed in 2560 1920 size that have overlays or scales etc placed on top of them If you selected 2560 1920 for the image size it will be automatically reduced to 1280 960 The TOOL menu cannot be used in C Scan mode The TOOL menu is disabled during electronic zoom The contents you drew or entered from the TOOL menu are hidden when you turn on the electronic zoom The hidden contents can be redisplayed af...

Page 71: ...sure Measures the distance between two points Scale Displays a scale C Scale Displays an XY scale Cross Crosshairs Cross Display Color Changes color or shape Set Changes display mode and saves settings Superimposition Superimposition Create Create Disp Display Info Information display Clear All Erases overlay information Overlay ON OFF Displays or hides overlay Pen Pen function Marker Count marker...

Page 72: ...verlay is simply held in temporary storage so its layout will not be fixed as valid data unless you paste it to the image If you click to return without pasting the content you ve drawn will be lost Once the overlay is fixed you can choose to display or hide it by using Overlay ON OFF Displaying or hiding the overlay Overlay ON displays the overlay on top of the screen Overlay OFF hides the overla...

Page 73: ...s saved or printed If you want to save an overlay by writing it over the image we recommend saving it in an image size of 1280 960 For a 640 480 image size it is possible that part of the text will be dropped Furthermore if the image is saved in JPEG format we recommend selecting a low compression rate such as Fine or Normal For details refer to 1 4 2 Image File Settings File Settings in Chapter V...

Page 74: ...use to use this function 1 Press Pen The tool bar shown below will be displayed 2 Press the red blue green black white or yellow color icon to determine the color of the pen 3 Click at the starting point and hold down the mouse button while you drag and the locus of the pointer will be drawn If you failed to draw use Clear to erase the locus you ve now entered In this case what you ve drawn with t...

Page 75: ...nts independently for each color Or you can return to one of the colors you previously used and continue counting from the last count of that color If you failed to mark any point you can use Undo or Redo to cancel or redo your most recent action Up to 10 recent actions can be retrieved Use Clear to erase all the content you ve now entered All markers you ve attached will be erased unless you have...

Page 76: ...CL to erase all characters or BS to erase one character To enter characters from your keyboard move the cursor to the text input window and press the Enter key Use the SHIFT Backspace keys on the keyboard to erase all characters or the Backspace key to erase one character Type your comment on the keyboard and hit the Enter key to confirm the content of your comment The Input Mode message disappear...

Page 77: ...ter over one of numbers s1 through s7 and the calibration information for that number will be displayed in the Info area for your confirmation 2 Press Calib to bring up a calibration screen like the one shown below 3 Draw a calibration line about the length of a specific subject on the image that you want to be the reference Click the mouse button at the starting point of the object to be measured...

Page 78: ...umeric value input box to display the numeric keypad Use the keypad to enter the magnification of the relay lens 5 If you want to attach information to the calibration value you ve created click the Info input box and enter text for the information you want to attach This information will prove helpful because you can confirm Info when you call up the calibration value at a later time 6 Check the ...

Page 79: ... is the Nikon 80i 90i microscope with motorized or intelligent nosepiece note that each calibration value call number is linked to the hole number of the nosepiece This means you cannot change calibration values by selecting s1 through s7 Calibration values are automatically changed when you change the objective 3 4 3 Measuring the Distance between Two Points You can measure the distance between t...

Page 80: ...e in the middle of Undo or Redo recent measurement lines drawn from that point in time cannot be canceled 4 To save a measurement line to the overlay of the image press Paste to fix after drawing it 5 Press to return to the menu in which you previously were Any measurement lines you ve created will be erased unless you pasted Once the content is fixed as valid you cannot erase it unless you erase ...

Page 81: ...crosshairs and a center only crosshairs The color can be selected from seven colors red green cyan yellow magenta white or black 5 Press Set to bring up the tool shown below This tool allows you to select a display mode for the crosshairs that suits the subject whose position you want to adjust 6 Radius is an option that displays a concentric circle along with the crosshairs with respect to its ce...

Page 82: ...ages NOTE If while crosshairs are on you create or display a superimposed image the crosshairs will be hidden The crosshairs can be redisplayed after you have finished creating or displaying a superimposed image Photographed images whose image size is 2 560 x 1 920 cannot be saved or printed with crosshairs written over the image If 2 560 x 1 920 is selected for the image size it will be automatic...

Page 83: ... 1 920 cannot be saved or printed with scales written over the image If 2 560 x 1 920 is selected for the image size it will be automatically reduced to 1 280 x 960 Use this function when the units nm µm mm and cm are displayed If other units are displayed you need to make the calibration setting once again If you perform an electronic zoom while using this function the screen display will reflect...

Page 84: ...ble to display XY scales depending on the selected calibration value If XY scales cannot be displayed a message C Scale NG is displayed in the Info area In that case change the calibration unit that has been set for example from 1 mm to 1000 µm Photographed images whose image size is 2 560x 1 920 cannot be saved or printed with scales written over the image If 2 560 x 1 920 is selected for the ima...

Page 85: ...e screen 1 Press Superimposition Create The tool bar shown below will be displayed with a yellow border displayed on the screen 2 Move the mouse pointer into the area and click it while you drag in order to specify the range in which you want to create a superimposed image 3 Press Create and the system will start creating a superimposed image automatically This will take some time Depending on the...

Page 86: ...le part of the screen displayed before being split moves to the left to become a still or frozen image Any part of the screen that has disappeared as a result of this operation can be made to reappear by scrolling with a mouse Move the mouse pointer to the boundary between the visible and the hidden part of the screen and it will change shape to become a cross hair pointer Now click the mouse butt...

Page 87: ...a network Possible operations in each method are described below The symbols and in the diagrams below indicate the flow of images while the symbol indicates the flow of control Using a WEB browser to operate the DS L1 from a PC Get images from the DS L1 Control the DS L1 in a simple way A WEB browser may be used from a LAN based PC to observe images It is possible to view small images with a rapi...

Page 88: ...mands may be used from a PC to achieve advanced control of the DS L1 In this case the DS L1 s Telnet server function is used Operating on a PC from the DS L1 Send images to an FTP server Images can be transferred to a specified FTP server NOTE This function cannot be used unless an FTP server facility is available on the PC side Because up to five FTP servers can be specified images can be easily ...

Page 89: ...corner of the Network Settings screen of the SETUP menu In automatic IP address acquisition by DHCP the assigned IP addresses change dynamically Accordingly if you are using a web browser to access the DS L1 from a PC we recommend setting a fixed IP address for the DS L1 Confirming the PC to be network connected OS of the PC used for a Web browser and browser versions There may be slight differenc...

Page 90: ... L1 If you are going to connect the DS L1 to a LAN you need to enable it for network operation and set its own address To make network settings on the DS L1 select Network Settings from the SETUP menu to call up the Network Settings screen shown below Enable Network To use a network always select this check box Read Only FTP WEB disable Write Control This is to disable writing to the internal file...

Page 91: ...address value hold down Skip on the screen keyboard while you skip to the last entry of the address then reenter the correct value Once you have finished with all the settings press SAVE and then restart the DS L1 To revert to the initially set value press Default and then SAVE and restart the DS L1 Settings for improved security To improve security make the following settings on the Network Setti...

Page 92: ... L1 and of time information When accessed from a WEB browser access logs are kept only when the user IDs have been set in the Internal Server on the DS L1 side However these logs disappear when the DS L1 is powered off Run the Telnet command w_NLGON and the initial settings can be changed to display NETLOG To get the data contained in the logs access the DS L1 using an FTP command and get and read...

Page 93: ...ernet Explorer 1 Start the PC Refer to your computer s user manual for details 2 Launch Internet Explorer Refer to your computer s user manual or the Internet Explorer help function for details 3 Select Tools Internet Options 4 Select Connections 5 Select LAN Settings Left click here to open the pull down menu Left click here Left click here Left click here ...

Page 94: ...IP address of the DS L1 Refer to 4 2 2 Setting Essential Items in Chapter VI for details on the IP address of the DS L1 Enter a period after every three digits of the IP address If the first digits of the IP address are 00 or 0 do not enter the zeros 8 Click OK Move the pointer to the position of OK and left click on it Repeat this until the screen returns to the one from step 1 Left click here En...

Page 95: ...anual for details 2 Launch Netscape Refer to your computer s user manual or the Netscape help function for details 3 Select Edit Preferences 4 Select Advanced Proxies Lift click Advanced When a menu appears under Advanced left click on Proxies Left click here Left click here to open the pull down menu and select Preferences from the menu ...

Page 96: ...ng Essential Items in Chapter VI for details on the IP address of the DS L1 Enter a period after every three digits of the IP address If the first digits of the IP address are 00 or 0 do not enter the zeros 7 Click OK Move the pointer to the position of OK and left click on it Repeat this until the screen returns to the one from step 1 Left click here Left click here Enter the IP address here Exam...

Page 97: ...m image ROOM2 Allows you to load saved image files from a CF card into the PC If multiple PCs simultaneously access one DS L1 through a WEB browser operation may slow down In the case of the simultaneous access from only two units of PC the operation should be comfortable NOTE The screen shots shown in this section may differ depending on which browser software or version is used Regarding the use...

Page 98: ... Explorer or Netscape Communicator on your computer 2 Enter the IP address of the DS L1 in the URL address field Example http 192 168 10 2 The Entrance screen of the DS L1 will appear 3 Click either ROOM1 or ROOM2 ROOM1 and ROOM2 are explained in more detail in the subsequent pages Click either ROOM1 or ROOM2 ...

Page 99: ...rance screen Reload Redisplays the ROOM1 screen with the preview image updated ROOM2 Displays the ROOM2 screen 2 Stream Window Displays a stream image subwindow in which a still picture is successively displayed as a stream image To close this window click the x button at the upper right corner This window is automatically closed when you go to another screen Entrance or ROOM2 Method for displayin...

Page 100: ...ge size you want to download and the latest image in that size will be downloaded into the PC in BMP format 1 When you click any image size to select Save it to disk a screen is displayed for you to select the folder in the PC that you wish to save to 2 Choose the folder in the PC where you want to save the image file Enter a file name as necessary and click the Save button When you click the Save...

Page 101: ...PEG format If you select Save As in the displayed menu the screen shown below will be displayed 2 Select the folder in the PC where you want to save the image file Enter a file name as necessary and click the Save button When you click the Save button the image that is in the DS L1 is downloaded into the PC Because the latest image is downloaded it may not always the same as the preview screen Not...

Page 102: ...image disappear with the preview screen displayed in normal size If high picture quality 1280 x 960 is selected for the picture quality of the preview screen a middle quality 640 x 480 image is displayed in an enlarged view 9 Location The DS L1 installation information etc that has been set by Telnet commands is displayed here Settings by Telnet commands Connect from an external PC to the DS L1 by...

Page 103: ... the folder at the level directly below it and the JPEG format files and BMP format files in that folder all are displayed 5 Files in the CF card Shows the file names created date time and log comments of the JPEG format files and BMP format files in the CF card The log comments displayed here are the contents of the log text that you attached from the REC menu by selecting Append when saving imag...

Page 104: ...rch for a directory named RAMIBOX 3 Execute the cd RAMIBOX command Four file names that are virtual names will be displayed IMGMID bmp 640 x 480 bitmap IMGMID jpg 640 x 480 JPEG IMGFULL bmp 1280 x 960 bitmap IMGFULL jpg 1280 x 960 JPEG The time of day for the files displayed at this time is 0 hours 0 minutes The actually acquired files are given the time of day when you issued the Get command 4 Ex...

Page 105: ... been set up in a unique manner so invalid operations could cause a problem on your network Enter the IP address of the DS L1 from the MS DOS prompt command prompt of Windows to connect to the DS L1 Example Telnet 192 168 10 2 Commands W_LCA_ space 32 characters Allows you to register the Location information to be displayed on the WEB screen W_IPA_ Allows you to register the connectable IP addres...

Page 106: ... L1 you need a PC which has FTP server functions Only basic settings are described here Refer to your OS s help function and other materials for more detailed information on settings 4 6 1 1 When Using Windows NT Workstation Ver 4 0 Setup procedure 1 Install Microsoft Peer Web Services Add FTP server functions 2 Add a user User ID and Password input Set the DS L1 as the login user 3 Specify the ho...

Page 107: ...les are to be transferred Also select the Write check box to enable writing to the home directory When image files are transferred the FTP Dir specified on the DS L1 side is created at a location below this home directory and the image files are saved in that directory Example If the home directory is C DSFTP and the FTP Dir specified on the DS L1 side is FTPIBOX then the FTP transfer directory is...

Page 108: ... DSFTP DS2 and C DSFTP DS3 That way you can specify a directory separately for each user 4 6 1 2 When Using Windows 2000 Professional and XP Professional The following explains how to set FTP functions in Windows 2000 Professional and XP Professional The Control Panel is displayed in a different way on the two operating systems In Windows 2000 you select Start Settings Control Panel In Windows XP ...

Page 109: ...sword To ensure that the same information has been set for External Server in Network Settings of the DS L1 enter the user ID and the password within ten alphanumeric characters in lower or uppercase Deselect User must change password at next login check box NOTE You also can create accounts and set passwords from Control Panel Users and Passwords or User Accounts for XP Specifying the home direct...

Page 110: ...Sites properties window Be aware that if you select the Allow Only Anonymous Logon check box no one but an anonymous user can log on When specifying a directory in which to save image files separately for each of multiple DS L1s connected 1 Select Computer Management Local System Tools Local Users and Groups Users 2 Double click the user you want to set and specify a local path for the home folder...

Page 111: ... the built in Ethernet through Apple Logo System Environment Settings Network Settings On home directory settings and the separation of save directories for each of multiple users DS L1s When a user is added a home directory is automatically created under the specified user name and directory name at a location below Macintosh HD Users When image files are transferred the FTP Dir specified on the ...

Page 112: ...irmation can also be accomplished by selecting Macintosh HD Applications Utilities Network Utility The Info tab Shows the IP address and other information The Ping tab Sends a ping command To use a function that is equivalent to the command prompt in Windows select Macintosh HD Applications Utilities Terminal to display the Terminal screen ...

Page 113: ...belongs to a different network group you must enter the gateway settings Server No Five servers can be stored in memory Assign each server a number IP Address 192 168 010 001 Input example User ID Enter a user ID that is needed to log in to the server Pass Word Enter a password that is needed to log in to the server as necessary Gate Way 192 168 128 001 Input example If any entry in the IP address...

Page 114: ... to for reasons of security functions such as packet filter firewall or security gateway Ping test may be reexecuted after disabling the security function of the server In that case restart the DS L1 first Also even when Ping was not responded to there is no problem with the server connection only if the server can be logged in in the login test described below Login test To test logging in to the...

Page 115: ... file is automatically assigned a file name according to settings on the DS L1 side The folder names and file names are set in File Settings of the SETUP menu For file names we recommend selecting Auto for File Name so that files will be named after the dates and times at which they are saved to the FTP server When images are sent via FTP the DS L1 does not check for duplicate file names Although ...

Page 116: ...at given intervals The following explains how to save images from the REC menu Configuration of the REC menu NOTE Refer to 2 2 1 Saving an Image to a CF Card REC Menu in Chapter VI for details on how to set in the REC menu Press CF to save an image to the CF card 1shot Only one image is saved Continue Interval photography photographed at given intervals Select the CF FTP check box and an image wil...

Page 117: ...that files will be named after the photographed date and time 1 Press 1shot to change to Continue The Cnt and Int icons will be displayed 2 In Cnt set the number of frames to be photographed Up to 500 frames can be set If Endless is selected in Cnt as the number of frames an image will be photographed successively 3 In Int set a photography interval time It can be set anywhere from 10 seconds to 6...

Page 118: ...wise data may not be read or written to correctly About the recording media used by the DS L1 Usable media Compact flash card Type I and II microdrive 1 GB Media formatting When formatting media in your PC make sure it is formatted in FAT12 16 Sometimes these are expressed simply as FAT or in FAT32 Note that depending on the OS used you may not be able to select a format The DS L1 has a function f...

Page 119: ...d that the interface board and the DS L1 be connected in only one hub stage and not in cascaded hub stages NOTE When multiple units of DS L1 are connected to a Windows 2000 machine equipped with a USB 1 1 interface board or a USB 1 1 hub the DS L1 units do not operate normally If connecting multiple units of DS L1 is desired use a USB 2 0 interface board and a USB 2 0 hub or use Windows XP for the...

Page 120: ... Update Web site Visit the Microsoft website for details on how to download drivers To install the USB 2 0 host driver that you have downloaded read the manual supplied with your interface board or consult the manufacturer 5 2 Connecting the DS L1 and a PC Follow the procedure described below to connect the DS L1 and a PC 1 Turn on the power to the PC main unit and start Windows 2000 or Windows XP...

Page 121: ... Installing the Device Driver When the DS L1 is connected to a PC for the first time the PC automatically recognizes the DS L1 and installs the device driver that comes standard with the OS During the installation process the window shown below will be displayed sequentially You do not need to perform any specific operations ...

Page 122: ...rrectly into position the message Please insert media is displayed when you click the icon for the media drive A similar message is displayed when you click the icon for the CCD image drive Connecting the DS L1 will immediately disable all other USB devices connected to the PC In such a case change the drive letter assigned to the media drive or the CCD image drive The following shows how to make ...

Page 123: ...disk icon on the My Computer screen to display its Properties dialog box This dialog box helps you identify the drive assigned to that removable disk In the case of the CCD image drive for example it can be identified based on the volume label NIKONIMG and its capacity 16 0 MB as shown below NOTE Note that because this drive is write protected its volume label cannot be altered ...

Page 124: ...ed from the PC to other media files in the same way as files are normally manipulated on a PC Removing media When the DS L1 is connected to a PC follow the procedure described below to remove the media from the media drive 1 Check to see that the PC card indicator on the DS L1 is turned off Close the menus displayed on the DS L1 2 If any image files have been loaded from the media into the PC clos...

Page 125: ...ls IMG640 BMP 2560 x 1920 pixels IMG2560 JPG 1280 x 960 pixels IMG1280 JPG JPEG files 640 x 480 pixels IMG640 JPG Refer to 1 4 2 Image File Settings in Chapter VI for details on file settings NOTE To capture an image file of 2 560 x 1 920 pixels besides file setting the display mode must be set at 5Mi Note that when the display mode is set at 1 3Mp or 1 3Mi an image of 1 280 x 960 will be captured...

Page 126: ...irming the updated date time of image files 1 Open My Computer and display image file icons Then select View Refresh to update the image file icons displayed 2 Right click any image file icon of interest and select Properties from the ensuing menu The updated date and time will be displayed ...

Page 127: ... if the PC remains powered on However to prevent data from being corrupted by removing the DS L1 during write operation follow the procedure described below 1 Eject the media from the media drive before removing the DS L1 2 Unplug the USB cable or turn off the power to the DS L1 3 When the below is displayed click OK Plug in the USB cable and turn on the DS L1 The device will be recognized by the ...

Page 128: ...rocess messages will be displayed sequentially in the task tray system tray at the lower right corner of the monitor screen indicating that new hardware has been detected You do not need to perform any specific operations When the Safely Remove Hardware icon is displayed in the task bar it means the PC has finished installing the driver When the DS L1 is connected to a USB 1 1 compliant USB port t...

Page 129: ...K button When you click OK button the Explorer screen opens showing the content of each drive NOTE In the following cases there might be a conflict between another connected device and the drive letter When media is inserted correctly into position the message Please insert media is displayed when you click the icon for the media drive A similar message is displayed when you click the icon for the...

Page 130: ...able Storage on the My Computer screen to access the media The image files on the media drive can be retrieved from the media or transferred from the PC to other media files in the same way as files are normally manipulated on a PC Removing media 1 Check to see that the PC card indicator on the DS L1 is turned off Close the menus displayed on the DS L1 2 If any image files have been loaded from th...

Page 131: ...pixels IMG640 BMP 2560 x 1920 pixels IMG2560 JPG 1280 x 960 pixels IMG1280 JPG JPEG files 640 x 480 pixels IMG640 JPG Refer to 1 4 2 Image File Settings in Chapter VI for details on file settings NOTE To capture an image file of 2 560 x 1 920 pixels besides file setting the display mode must be set at 5Mi Note that when the display mode is set at 1 3Mp or 1 3Mi an image of 1 280 x 960 will be capt...

Page 132: ...irming the updated date time of image files 1 Open My Computer and display image file icons Then select View Refresh to update the image file icons displayed 2 Right click any image file icon of interest and select Properties from the ensuing menu The updated date and time will be displayed ...

Page 133: ...DS L1 during a write operation follow the procedure described below 1 Eject the media from the media drive before removing the DS L1 2 Click the Safe to Remove Hardware icon on the task bar 3 Drives should be selected one at a time First select the media drive If the media drive can be removed the message shown below is displayed 4 To remove the CCD image drive follow step 3 5 Unplug the USB cable...

Page 134: ...roscopy setup menu MIC Info menu is not displayed Before using microscope control function from DS L1 you must register the microscope status from the PC using the setup software Consult the instruction manual for your microscope 6 2 Menu Overview When you open a menu while the microscope is connected to your system the menu displayed on the left side of the screen for default settings will have t...

Page 135: ...yed on the DS L1 menu are limited to those for parts whose status can be detected electrically among the various parts of your microscope Keep in mind that the items displayed on the DS L1 menu do not include those for parts whose status cannot be detected electrically Refer to the table below for the relationship between the microscope system and menu display 1 When the Nikon 80i microscope incor...

Page 136: ...er Control status display menu displayed 3 When the Nikon 90i microscope without DIH is connected Parts Menu displayed Focus Z axis Control status display menu displayed Nosepiece Motorized nosepiece Control status display menu displayed Optical zoom No menu displayed Optical path No menu displayed Shutter No menu displayed Filter cube No menu displayed Lamp Control status display menu displayed M...

Page 137: ...y menu displayed Lamp Control status display menu displayed Motorized universal condenser Control status display menu displayed Universal condenser Non motorized universal condenser No menu displayed With motorized diaphragm Control status display menu displayed Diascopic illumination aperture diaphragm Without motorized diaphragm No menu displayed Diascopic illumination field diaphragm Control st...

Page 138: ...isplayed Lamp Control status display menu displayed Motorized universal condenser Control status display menu displayed Universal condenser Non motorized universal condenser No menu displayed With motorized diaphragm Control status display menu displayed Diascopic illumination aperture diaphragm Without motorized diaphragm No menu displayed Diascopic illumination field diaphragm Control status dis...

Page 139: ...figuration when the microscope connected has all motorized parts attached Select this check box when you want to connect the camera head to the front port of the DIH Focus Coarse fine selector Fine motion Coarse motion Escape The stage descends for specimen replacement Autofocus Objective Shows magnification and NA value Shows detail information Selects the objective to use Optical zoom Changes th...

Page 140: ...nu Using the autofocus function When this function is selected focus is adjusted automatically Before using this function please read the separate manual entitled 90i Reference Autofocus Also refer to Section 6 3 Autofocus Precautions in this manual 1 Select the autofocus AF icon 2 The message Now Focusing will be displayed at the upper right part of the screen indicating that the autofocus functi...

Page 141: ...ed nosepiece 1 In the nosepiece column select the nosepiece number corresponding to your desired objective The objective is switched to the one that is attached to the nosepiece number you ve selected 2 After switching objectives confirm that the selected number icon is marked with a red border and that the nosepiece number the objective magnification and NA value are displayed NOTE Before this fu...

Page 142: ... select the icon in the optical zoom column Conversely if you want to increase the magnification select the icon to change the magnification NOTE For the microscopes incorporating the DIH M only the magnification is displayed The magnification changes in increments of x0 1 and can be changed in the range x0 8 to x2 0 The current magnification is displayed on the microscope status display microscop...

Page 143: ...ted microscope incorporates the DIH M or DIH E When the DS L1 is used with the front port you will see the message None displayed in the Zoom column of the microscope control menu 1 and the microscope status display microscopy setup menu MIC Info menu Information on the selected port to which the camera head is connected is retained even after power to the camera head is turned off The saved setti...

Page 144: ...t the icon is marked with a red border To turn the lamp off 1 When the lamp is on select the icon to turn the lamp off 2 While the lamp remains turned off the icon is marked with a red border Adjusting the brightness of the lamp 1 When the lamp is lit select the icon and the lamp will become brighter in increments of 0 1 V Conversely when you select the icon the lamp becomes darker in increments o...

Page 145: ...ents are missing or manual parts are attached no menus will be displayed for those components Menu configuration when the microscope connected has all motorized parts attached Episcopic illumination field diaphragm Adjusts the diaphragm Condenser Shows condenser module name Selects the condenser module you want to use Diascopic illumination aperture diaphragm Adjusts the diaphragm Diascopic illumi...

Page 146: ...condenser from within this display to change condensers Diascopic illumination aperture diaphragm Adjusts the diascopic illumination aperture diaphragm Compatible only with the Nikon 90i microscope 1 In the diascopic illumination aperture diaphragm column select the icon to open the diaphragm in increments of 0 1 Or select the icon to close the diaphragm in decrements of 0 1 2 Briefly depress the ...

Page 147: ...r Adjusts the transmission factor of the ND filter Compatible only with Nikon 90i microscopes with motorized ND filters attached 1 In the ND filter column select the icon to increase the transmission factor by 3 relative to the current set value Or select the icon to reduce the transmission factor by 3 relative to the currently set value 2 Briefly depress the mouse button to change the transmissio...

Page 148: ...on and NA value Optical zoom Shows the optical zoom magnification when connected to the rear port of the DIH Info box information display indicates operating status or detailed items for the selected icon Stage position Z axis Shows address along the vertical axis Z axis Observation method Sets the microscope for bright field microscopy Sets the microscope for differential interference contrast mi...

Page 149: ...ddress along vertical axis Compatible only with the Nikon 90i microscope 1 The menu indicates the height of the current Z focus position i e position along the Z axis NOTE Use microscope control menu 1 to adjust focus Optical zoom Shows the optical zoom magnification Compatible only with microscopes incorporating the DIH 1 If the camera head is connected to the rear port of the DIH the menu indica...

Page 150: ...s for this observation method 2 The icon representing the current observation method will be marked with a red border NOTE The default observation method on startup is BF Right click on any icon representing an observation method to display the names of all observation methods You also can select the desired observation method from within this display to change observation methods To set the micro...

Page 151: ... the Microscope Control 143 To link the observation method with the photographing mode of DS L 1 Right click on the icon indicating an observation method to open the menu showing all observation methods 2 Enter a check mark in the check box ...

Page 152: ...8037 Cover glass Complies with JIS R3702 or ISO 8255 JIS R3702 Thickness 0 12 0 17 mm for No 1 0 15 0 18 mm for No 1 S ISO 8255 Thickness 0 13 0 17 mm for No 1 0 15 0 17 mm for No 1 H 2 The autofocus function may force contact between the sample and the tip of the objective resulting in damage to both For samples like the ones shown below adjust focus with the focusing handle rather than relying o...

Page 153: ...e ones indicated below Low contrast samples Samples densely dyed dark over the entire view field Samples prepared for methods other than bright field microscopy Samples that lack light and dark patterns running horizontally along the screen lateral direction in the diagram shown at right 2 Since autofocus responds to the highest contrast part of the sample the focused position of the sample may no...

Page 154: ...g conditions set on the DS L1 when the sample is nearly in focus Scene mode BF Exposure time Under AE setting the exposure time should come within 1 120 to 1 350 in 1 3 MP display mode when the sample is nearly in focus Adjust with ND filters etc Warn Exp Under Microscopy condition is too dark Focusing stops at the original position for the autofocus Use the autofocus function under following cond...

Page 155: ... the front of the CCD If you do touch the glass filter or if dirt cannot be removed from it contact your nearest Nikon representative and have them clean it Do not use organic solvents e g alcohol ether or paint thinner to clean the coated plastic or printed parts Doing so could result in discoloration or in separation of the printing 2 Storage Turn off the power when the system is not in use If t...

Page 156: ...ome on Make sure the power cord is plugged firmly into an AC outlet at one end and the DS AC adapter and then the DC IN connector on the DS L1 main unit on the other Use the power cord supplied with the system Turn on the POWER switch The main unit is hot to the touch The main unit temperature may increase when used in a hot or confined space If the high temperature is accompanied by a burnt smell...

Page 157: ...or an External Monitor in Chapter VI Only a crossline image appears on a white screen Connect the camera cable securely The image is out of focus Set Sharpness on the CAM menu appropriately See 2 Photographing Images in Chapter VI Turn off the electronic zoom function Focus the microscope or lens correctly Do not use the system in a vibration prone place Adjust the aperture diaphragm correctly The...

Page 158: ...t the size of the field diaphragm so that it circumscribes the viewfield Adjust the size of the aperture diaphragm to approximately 70 to 80 of the numerical aperture of the objective Use an appropriate filter When using a phase contrast microscope or an interference microscope two luminous fluxes or multiple luminous fluxes insert the specified filter With a black and white image contrast is impr...

Page 159: ...th an unintentional change of camera modes set SOURCE to S1 3M in File Settings of the SETUP menu See 1 4 2 Image File Settings in Chapter VI 4 Abnormal Behavior Symptom Corrective action The system s internal clock does not keep time correctly If the system is not used for an extended period of time the backup function deteriorates causing the clock to stop Simply reset the correct time The backu...

Page 160: ...that crossed cables are used Checking hub connections See the instruction manual supplied with the hub for details and follow the description written in it Do not connect the DS L1 to the Uplink Be aware that Uplink on some hubs is indicated as MDI Uplink 1 1 1 or 8 8 Also if a hub has cross and straight ports caution must be exercised There is a limited number of useful ports on a hub Be careful ...

Page 161: ...eturned the presence of the DS L1 is not recognized Check the cables hub connections and the network settings of the PC e g the IP address Also check that the PC is connected to a LAN by using a browser or the ping command Browser Cannot access the camera from a browser Set exception items in the browser s proxy settings Cannot control the camera from a browser Deselect the Read Only check box in ...

Page 162: ...heck the network cable and hub connections Select the Enable FTP Client check box in Network Settings of the SETUP menu Even though the FTP send button is pressed the timer icon keeps running for about 1 minute and no images are sent This is because the IP address of the External Server that has been set in Network Settings of the SETUP menu cannot be found Check the IP address of the server one m...

Page 163: ...luded with the USB board does not work properly When images are successively downloaded it is the same image that appears on PC Images are updated once every 5 seconds Be aware that if files are loaded into a PC at intervals shorter than 5 seconds the captured image may not always be the latest one Warnings are displayed on the PC A warning in the task tray system tray such as HIGHT SPEED USB Devi...

Page 164: ...ted in the Additional Settings screen in the SETUP menu Unable to click the printing icon Confirm that a PictBridge supporting printer is connected to the USB D connector on DS L1 Confirm that the power for the PictBridge supporting printer is turned on In the case that it is still unable to click the printing icon even though the power is turned on close the menu turn on and off the power once mo...

Page 165: ...ely between the DS L1 to the digital imaging head on the 80i or to the 90i microscope Confirm that only one hub stage exists between the PC s USB port and the DS U1 We strongly recommend against using more than one hub Use the USB cable supplied with the microscope to connect the microscope to the DS L1 Check whether the Nikon 80i or 90i microscope indicates an abnormal state by buzzer or LED disp...

Page 166: ...00 to 600 seconds for DS Cooled Camera DS 5Mc Light source color temperature range 2800K to 6800K White balance Set method color balance adjustment Color correction Contrast black level saturation hue adjustment monochrome inversion other special effects are selectable Image correction Shading correction noise reduction horizontal vertical inversion of image Operation Scene mode Selectable from a ...

Page 167: ... client General External dimensions 203 W 202 H 73 D mm not including protrusions Weight 1 3 kg Rated input voltage 12 VDC 5 Power consumption Approx 138 VA Structure of fixed part VESA 75mm standard 75 mm pitch M4 7 fixing screw holes 4 locations UL1678 suited stand and mount 2 DS Camera Head DS 5M Image pickup device 2 3 inch square 5 240 000 pixel IT CCD Frame rate 3 75 fps 5Mi 7 5 fps 1 3Mp 6 ...

Page 168: ... Telecentric system F2 8 Image circle 2 3 inch 11 mm in diameter External dimensions 91 W 76 D 41 H mm Weight 290 g Fixing tripod mount Tripod screw for camera CCD cooling Cooling with Peltier device Ambient temperature 20 C 4 DS Remote Controller DS RC External dimensions 56 W 68 D 21 H mm Weight 110 g 5 DS AC adapter Rated input voltage 100 240 VAC 50 60 Hz 1 2 A max Rated output voltage 12 VDC ...

Page 169: ...he EU Low Voltage Directive This equipment has been tested and found to comply with the specifications for a Class A digital device pursuant to Part 15 of the FCC Rules These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment This equipment generates uses and can radiate radio frequency energy and if not inst...

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