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10. Software STEVE
Selecting the mounting medium‘s refractive index
How to select the mounting medium’s refractive index
Before either a single-shot or a time-lapse acquisition is started, a dialog will appear in STEVE (Figure 23) prompting you to select the mounting
medium refractive index (e.g. DMEM, Figure 24).
You may select the mounting medium from the drop-down list of commonly used media.
If yours is not listed you may add it to the list by clicking “New” and entering the new mounting medium refractive index and its name (Figure 25).
In case you are using an aqueous mounting medium not found in the list for which you do not know the refractive index, it is safe to assume it
is close to water. If using oil mounting media however, it is important to specify a correct refractive index as it can be significantly larger than of
aqueous solutions.
Figure 23 Mounting medium Refractive Index
Selection Window
Figure 24 Example Mounting Medium Water
Figure 25 „New“
