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4. Technical Specification Sheet (TSS)
3D Cell Explorer-
fluo
& CoolLED illumination system
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Specification 3D Cell Explorer-
fluo
/
Holotomography
Specifications CoolLED illumination system /
Epifluorescence
Dimensions (width x depth x height in mm)
380 x 170 x 445
77 x 186 x 162
Weight (in kg)
8
4
Environmental Operating Conditions
Permissible ambient temperature, and can be
operated between 20° C and 30° C
5-35°C
Voltage ranges
100-240 VAC / 50-60 Hz / 1.0-0.5 A
110-240 VAC / 50-60 Hz / 2 A
Camera
USB3.0 CMOS Sony IMX174 sensor
Quantum Efficiency (typical) 70 % (at 545 nm)
Dark Noise (typical)
6,6 e¯
Dynamic Range (typical)
73,7 dB
Microscope Objective (MO)
Dry objective / 60x magnification / NA 0.8
Illumination Source
Class I laser low power (
λ
=520 nm,
sample exposure 0.2 mW/mm
2
)
(Class I. No eye protection needed)
High speed switchable <100 μs
Lifetime >20’000 hours each channel
Field-of-View (FoV)
90 x 90 x 30 μm
3
90 x 90 μm
Lateral resolution
200 nm
400 nm
Axial resolution
400 nm
-
Tomography frame rate (acquisition)
0.5 fps
3 fps each channel
Self-adjusting time
< 90 seconds (Depending on sample mounting
medium thickness)
Channels
Up to 7 simultaneously
3: DAPI (blue), FitC (green), TritC (orange)
3: FitC (green), TritC (orange), Cy5 (red)
4: DAPI (blue), FitC (green), TritC (orange) and
Cy5 (red) (Note: TritC and Cy5 excitation filters
are exchangeable on channel 3)
