Operation
PC software
Confluence
1.
Click the confluence menu button.
2.
Select one image that needs recalculated confluence value
from data list.
3.
Adjust the image by changing parameters; recommended
sensitivity level and background level corresponding to cell line.
4.
Click the
Confluence button
. the value of confluence would
be recalculated by new sensitivity level, and background level.
5.
If the confluence value from step 4 is good enough to use,
then check
Apply box
for recalculating confluence value of all
images in same folder by new sensitivity and background level.
6.
Confluence graph would be redrawn.
7.
Click
Save as button
if recalculated data should be saved.
Parameter
A(Default)
B
C
Cell line
HepG2, GH3, Hep3B,
A549, MCF7, SH-SY5Y,
SCN2.2, NIH-3T3, F9,
HeLa, Du-145
LNCaP
U-2 OS, ADSC
Recommendation
Sensitivity level 9
Background level 2
Sensitivity level 10
Background level 2
Sensitivity level 10
<before>
<after>
Time ( Hour : Min )
Time ( Hour : Min )
46
