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NanoEnTek JuLi BR, User Manual

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NanoEnTek JuLi BR User Manual Download Page 18

Operation

Focusing

Confluence

6.  Check confirmation message ‘Capture complete’.  
Saved data can be opened in Data menu. 

(on page 20)

The images below show the guideline for focus to obtain 
accurate cell confluence.

Du-145   /   Parameter A

Good setting

Exposure 4 
Brightness 1

Normal setting

Exposure 6 
Brightness 3

Bad setting

Exposure 6 
Brightness 5

Confluence 35.11%

Confluence 36.22%

Confluence 43.03%

18

Summary of Contents for JuLi BR

Page 1: ......

Page 2: ...29 Rename 30 Delete 31 32 Counting 33 36 Settings 37 39 Set up parameter 37 Update date time 38 Control LED power 38 Update software firmware 38 Check the disk space 39 PC software 40 51 Introduction...

Page 3: ...your local distributor or e mail sales nanoentek com Model JULI BRSC Scope unit is only used in conjunction with the Station unit Scope unit JULI BRSC 1 EA USB drive 4 GB 1 EA Station unit JULI BRST 1...

Page 4: ...install the system in your cell culture incubator easily It can be used to compare control and experimental samples using dual systems optional concurrently Features of JuLI Br live cell movie analyze...

Page 5: ...T JuLI Br is optimized into standard size of cell culture incubator It is recommend that the temperature should be stabilized in case of small size incubator before using the instrument Avoid exposing...

Page 6: ...supplied connection cable For dual monitoring 2nd scope unit also should be connected to station unit 4 Plug the power cord into the electrical outlet Be sure to only use the power cord supplied with...

Page 7: ...functions and displays bright field images Scope unit Focus knob is used to adjust the image quality to obtain better bright cell images Stage place sample here White LED provides bright image viewer...

Page 8: ...t a USB drive to save and transport images to PC Display port connect to external monitor resolution 1366 X 768 pixels make sure to turn off an instrument before connecting Scope unit Connection port...

Page 9: ...nfiguration in your country On off switch the main power switch It is not necessary to use the on off switch for day to day operation of the instrument Connection port port to connect scope unit with...

Page 10: ...s proliferation assays Description User interface Focusing menu User interface Monitoring menu Zoom in Navigation Zoom out Auto focus Focus interface Exposure Brightness D Phase Capture Confluence Foc...

Page 11: ...on Zoom bar Multi select button Delete button Save to USB button Rename button Data menu Graph Data list Sensitivity Background level Disk space Software version Firmware version OS version LED power...

Page 12: ...etween large or small sizes Measures cell measurement ranging from 1 10 4 to 1 10 7 cells mL optimal measurement range from 1 10 5 to 4 10 6 cells mL and cells with sizes ranging from 5 m to 60 m Inno...

Page 13: ...elect each scope unit 4 To adjust Illumination intensity use the Exposure and Brightness bar on the touch screen Touch the arrow button for more minute adjustment 5 If desired use Zoom in button to ma...

Page 14: ...ob which moves up or down rapidly to get the sample into coarse focus Fine focus is smaller knob which moves short distances slowly and is used to get the sample into sharp focus The images below show...

Page 15: ...ndividual variations The Auto focus is the auto focusing function which automatically senses the focus optimized for the analysis software of the JuLI Br IMPORTANT It is strongly recommended to use Au...

Page 16: ...hand on the screen The ROI Auto focus is the auto focusing function which automatically senses the focus optimized for the analysis software of the JuLI Br IMPORTANT It is strongly recommended to use...

Page 17: ...try again after adjusting parameter in Setting menu on page 29 Parameter is the recommended value for corresponding to cell line If confluence should be recalculated press Confluence button after pres...

Page 18: ...pened in Data menu on page 20 The images below show the guideline for focus to obtain accurate cell confluence Du 145 Parameter A Good setting Exposure 4 Brightness 1 Normal setting Exposure 6 Brightn...

Page 19: ...ton to acquire the image 3 Type the name of image that should be saved Then press Save button 4 Check confirmation message Capture complete Press OK button 5 Saved data can be opened in Data menu on p...

Page 20: ...is connected to station unit 2 Adjust the focus of JuLI Br and select the region that should be monitored using Focusing menu on page 11 12 Press Setting button 3 Press the Name form to type file nam...

Page 21: ...file to create AVI file with time lapse images Select scope channel should be monitored Choose both channel 1 and channel 2 in case of dual monitoring IMPORTANT Warm up scope unit for 1 hour in incub...

Page 22: ...yed automatically when data value of Total Time and Interval are set up 6 Press the Apply button to save all options If the disk volume of the instrument is not enough to save recording data then the...

Page 23: ...expected problems such that the power is off suddenly or the scope unit is not connected to station unit while movie recording could be happened Then the error message as below would be shown after re...

Page 24: ...each of folder set up by user And other data except monitoring are saved in a default folder Captured Images 2 There are sub folders in case of dual monitoring data Each control sample and experimenta...

Page 25: ...shown by double tapping folder name in List category or tapping folder name once and pressing Result category 4 Recorded movie or image file can be shown by tapping its file name Movie file can be ch...

Page 26: ...5 Magnify movie screen or image screen widely for convenience Operation Data Open data 26...

Page 27: ...using moving icon 4 Select the screen size of snapshot using size icon Snapshot capture process can be canceled by pressing cancel icon 5 After then double tap the selected region 6 Type the name for...

Page 28: ...der or file by tapping it once 3 Several folders or files can be selected at once using Multi select button If the wrong file is selected press Multi select button again 4 Press the Save to USB button...

Page 29: ...te is shown saving process is completed Experimental sample data channel 2 is included in control sample data channel 1 folder in case of dual monitoring Operation Data Save to USB JuLITM Br Cell Hist...

Page 30: ...er Captured Images Not for each files in monitored data folder 2 Then press Rename button Only main folder can be renamed not sub folders individually in case of dual monitoring data 3 Enter new name...

Page 31: ...r files at once select all of them after pressing Multi select button 2 Delete the folder or file with Delete button If Captured Images folder is selected all of image files in the folder will be dele...

Page 32: ...4 Check confirmation message Delete complete Operation Data Delete 32...

Page 33: ...nting slide side A using a pipette The two chambers of the slide and labeled as A and B for easy tracking of your samples Each chambers is counted seperately 2 Put the counting slide with the slide ho...

Page 34: ...Br and provides the precise and rapid counting results The example images of HeLa cells show the reference calue for adjusting focus Operation Counting Auto focus Focus by user in various cases Dark...

Page 35: ...on is activated in the Bright mode If necessary set the dilution factor corresponding to the experiment condition Refer to page 42 3 The counting results including the total cell live cell dead cell a...

Page 36: ...e the images and the counting result file for one folder automatically In a folder there are one original cell image one cell counting image marked with different color for the viable and dead cells o...

Page 37: ...e back ground Increased sensitivity provides better recognition of fainter objects in bright field Background level decreased background level provides better recognition of fainter objects in bright...

Page 38: ...update with the latest software and firmware using update button If you have any questions about software updates contact sales nanoentek com 2 Visit JuLI Br website at www nanoentek com to download...

Page 39: ...ess Update button The update process takes a few seconds 6 After updating Press OK button to restart JuLI Br automatically Check the disk space of JuLI Br If no disk space left saved data should be de...

Page 40: ...nce of all images and edited movies To install PC software follow the directions as below Check to ensure that the PC is connected to Internet and then the PC software could be installed 1 Connect the...

Page 41: ...tallation 4 If you want to change installation folder click Browse and choose the location that you want 6 The computer activates the Installation of the software 7 If the installation is successful t...

Page 42: ...n guide When PC software program starts the interface as below image will be shown Home Zoom bar Image information Preview window Graph Data list Confluence Movie Open Save as Export Create dual movie...

Page 43: ...Home 1 Press Insert scale bar button to insert scale bar on viewing image if it is necessary Click Save with scale bar button to save image with scale bar information JuLITM Br Cell History Recorder...

Page 44: ...ta 1 Click the Open button Folders and files can be opened by dragging and dropping on List region directly 2 Browse the folder or files and select it 3 If data opened successfully data list could be...

Page 45: ...image and movie In case of dual monitoring data control sample folder and experimental sample folder can be checked easily using Channel tab 5 The image can be magnified by tapping zoom bar 6 Select...

Page 46: ...ckground level 5 If the confluence value from step 4 is good enough to use then check Apply box for recalculating confluence value of all images in same folder by new sensitivity and background level...

Page 47: ...h PC software Set the start point and end point by moving the bar 2 Check Apply box to edit the movie 3 Click Save as button if edited movie should be saved 4 Select the folder to save edited movie an...

Page 48: ...view menu button Click Dual view menu button 2 Summary of dual monitoring data will be shown 3 Individual movie files from control sample and experimental sample can be made into one movie using Expo...

Page 49: ...pe file name for merged movie and click Save button 5 Merged movie file will be created after merging process is done Operation PC software Dual view JuLITM Br Cell History Recorder 2012 NanoEnTek Inc...

Page 50: ...elect 2 different single monitoring data folder for making movie Selected movie files should have been monitored under the same interval and total time 3 Click Make New Folder button and type folder n...

Page 51: ...n folder can be exported 2 Select the folder where exported file should be saved Type the file name to save exported file 3 Exported file can be found in folder Operation PC software Export data JuLIT...

Page 52: ...JuLI Br does not need regular maintenance To troubleshoot problems with JuLI Br contact Technical Support page 59 IMPORTANT Never disassemble or service JuLI Br by yourself Unauthorized repairs may d...

Page 53: ...efully Eliminate any dust on culture dish Check for and remove any condensation on the lid of the culture dish Warm up the instrument during 1 hour before monitoring power should be on when warming up...

Page 54: ...ith time lapse images using JuLITM Br PC software program Download JuLI Br Update folder on NanoEnTek website www nanoentek com Check the files as below image Then download JuLI Br Update folder on Na...

Page 55: ...Install the codec provided by JuLI Br USB drive or NanoEnTek website Check the resolution of the outer monitor resolution 1366 X 768 pixels Make sure to turn off instrument before connecting PC softwa...

Page 56: ...e of fittings or spare parts supplied by anyone other than NanoEnTek 5 Damage caused by accident or misuse 6 Damage caused by disaster 7 Corrosion caused by improper solvent or sample For your protect...

Page 57: ...nto the air vents of the instrument as this could result in electrical shock personal injury and equipment damage Plug the power cord firmly into the wall outlet and AC adapter To avoid potential shoc...

Page 58: ...its are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment This equipment generates uses and can radiate radio frequency...

Page 59: ...RTB XY Stage Objective 4X and digital zoom 400X 2560 x 1920 pixels 5M JPEG image AVI movie CSV raw data 10 1 LCD touch screen White LED Scope unit 300 x 190 x 188 mm 4kg Station unit 282 x 285 x 160 m...

Page 60: ...and special offers For more information or technical assistance please call or email NanoEnTek Inc HQ 12F 5 Digital ro 26 gil Guro gu Seoul 08389 Korea Tel 82 2 6220 7940 Fax 82 2 6220 7721 NanoEnTek...

Page 61: ...F 5 Digital ro 26 gil Guro gu Seoul 08389 Korea Tel 82 2 6220 7940 Fax 82 2 6220 7721 The information in this manual is described as accurately as possible The changes involved in firmware and softwar...

Page 62: ...389 Korea Tel 82 2 6220 7940 Fax 82 2 6220 7721 NanoEnTek USA Inc 5627 Stoneridge Drive Suite 304 Pleasanton CA 94588 USA Tel 1 925 225 0108 1 888 988 0108 Toll free Fax 1 925 225 0109 Email sales nan...

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