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FastDNA SPIN Kit for Soil
®
The FastDNA -24 SPIN Kit for Soil quickly and efficiently isolates PCR-ready
genomic DNA directly from soil samples in less than 30 minutes. Designed for
®
use with the Fastprep instrument, plant and animal tissues, bacteria, algae,
fungi spores and other members of a soil population are easily lysed within 40
seconds.
Samples are placed into 2.0 ml tubes containing Lysing Matrix E (included in
the kit), a mixture of ceramic and silica particles designed to efficiently lyse all
soil organisms including historically difficult sources such as eubacterial
spores and endospores, gram positive bacteria, yeast algae, nematodes and
fungi.
®
Homogenization in the Fastprep -24 instrument with Lysing Matrix E takes
place in the presence of MT Buffer and Sodium Phosphate Buffer, reagents
carefully developed to protect and solubilize nucleic acids and proteins upon
cell lysis. These reagents work together to allow extraction of genomic DNA
with minimal RNA contamination.
Following lysis, samples are centrifuged to pellet soil, cell debris and lysing
®
matrix. DNA is purified from the supernatant with a silica-based GENECLEAN
procedure using SPIN filters. Eluted DNA is ready for PCR, restriction digest,
electrophoresis and any other desired application.
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