Molecular Devices versamax User Manual Download Page 1

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0112-0126 B
October 2018

SpectraMax® Plus 384
SpectraMax® 190
SpectraMax® 340PC384
VersaMax™

Microplate Spectrophotometers

User Guide

Summary of Contents for versamax

Page 1: ...0112 0126 B October 2018 SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC384 VersaMax Microplate Spectrophotometers User Guide...

Page 2: ...any right and or license to use or permit others to use such manufacturers and or their product names as trademarks Each product is shipped with documentation stating specifications and other technic...

Page 3: ...ter 4 Absorbance Read Mode 19 PathCheck Pathlength Measurement Technology 20 Read Types 24 Chapter 5 Maintenance 25 Cleaning the Instrument 25 Replacing Fuses 26 Before You Move the Instrument 28 Chap...

Page 4: ...sed in a manner not specified by Molecular Devices the protection provided by the equipment might be impaired Warnings Cautions Notes and Tips All warning symbols are framed within a yellow triangle A...

Page 5: ...ic Equipment WEEE Directive of the European Union It indicates that you must not discard this electrical or electronic product or its components in domestic household waste or in the municipal waste c...

Page 6: ...y harmful When you use such materials observe the following precautions Handle infectious samples based on good laboratory procedures and methods to prevent the spread of disease Observe all cautionar...

Page 7: ...red To prevent injury Never try to exchange labware reagents or tools while the instrument is operating Never try to physically restrict the moving components of the instrument Note Observe all warnin...

Page 8: ...ndpoint at a single point in time Kinetic over a specified period of time Spectrum over a specified wavelength range The shake feature enables you to mix the contents of the wells in a plate before ea...

Page 9: ...is not available for the VersaMax To run protocols that require advanced acquisition settings or to run Absorbance read mode Kinetic read type and Spectrum read type protocols you must connect the in...

Page 10: ...settings to shake the plate linearly along the long axis at preset intervals to mix of the contents of the wells Endpoint read type Settings enable you to shake the plate for a definable number of sec...

Page 11: ...s 384 where the instrument control panel displays the cuvette chamber temperature Use the SoftMax Pro Software to view the plate chamber temperature for the SpectraMax Plus 384 Typically the plate cha...

Page 12: ...te to prevent evaporation of the sample The seal or lid also helps to maintain a uniform temperature It can take an hour or more for a prepared sample to equilibrate inside the plate chamber You can s...

Page 13: ...omplete list of the package contents see the enclosed packing list The packaging is designed to protect the instrument during shipment Tape is placed on the cuvette door and the plate drawer to protec...

Page 14: ...witch that is on the rear of the instrument is in the Off position 2 To use a computer to operate the instrument connect the appropriate end of the supplied USB cable to the USB port that is on the re...

Page 15: ...the front of the instrument now faces you Note Ensure no cables run beneath the instrument 7 Remove the tape from the cuvette door on the SpectraMax Plus 384 8 Power on the instrument and wait for the...

Page 16: ...d Analysis Software User Guide When you connect the instrument to a printer and operate it in stand alone mode the instrument control panel enables you to run basic Absorbance read mode protocols The...

Page 17: ...nty SoftMax Pro Software Workflow For this workflow you connect the instrument to the computer on which you install the SoftMax Pro Software See the SoftMax Pro Data Acquisition and Analysis Software...

Page 18: ...r 2 Use the instrument control panel to set the wavelength and temperature for the read Wait for the plate chamber or cuvette chamber to reach the set temperature 3 Use the instrument control panel to...

Page 19: ...total quantity of light available Optical Density includes absorbance of the sample plus light scatter from turbidity and background You can compensate for background using blanks A blank well contain...

Page 20: ...distance of the light through the sample depends on the volume This variable pathlength makes it difficult to do extinction based assays and makes it confusing to compare results between microplate re...

Page 21: ...t concentration less than 0 5 M the Water Constant correction method is sufficient The Water Constant is determined for each instrument during manufacture and is stored in the instrument Cuvette Refer...

Page 22: ...thlength Measurement Technology feature after the read Eliminating Pathlength Independent Component Raw OD measurements of plate samples include both pathlength dependent components sample and solvent...

Page 23: ...highly colored in the upper visible spectrum might have absorbance that extends into the near infrared NIR spectrum and can interfere with the PathCheck technology Examples include Lowry assays molybd...

Page 24: ...Sec Time to VMax and Onset Time Kinetic reads can be single wavelength or multiple wavelength readings The kinetic read type can collect data points in time intervals of seconds minutes or hours up to...

Page 25: ...re applicable to do so If parts are returned they must be enclosed in a sealed plastic bag that states that the contents are safe to handle and are not contaminated WARNING BIOHAZARD Always wear glove...

Page 26: ...rument requires periodic cleaning The frequency of cleaning depends on the cleanliness of the lab and could range from once a month to once every six months 1 Remove the plate or adapter from the plat...

Page 27: ...at requires removal of a panel or cover or disassembly of an interior instrument component 1 Power off the instrument 2 Unplug the power cord from the power port 3 Use a small slot head screwdriver to...

Page 28: ...following applicable decontamination procedures and packing instructions Note If you must store the instrument then store it in a dry dust free environmentally controlled area Packing the Instrument...

Page 29: ...ory For assistance regarding warning messages contact your local Molecular Devices representative Error Code Classifications Not all error messages are listed here Error Code Ranges Possible Causes 10...

Page 30: ...d not perform the command because it was busy doing another task 106 command invalid measurement in progress Instrument could not perform command because a measurement was in progress 107 no data to t...

Page 31: ...ignoring flow control 203 serial port parity error Parity bit error detected with incoming serial data 204 serial port overrun error Caused by host computer sending too much data and ignoring the flow...

Page 32: ...tte door being open 305 reference level saturation During a cuvette read could be due to cuvette door being open 306 plate air cal fail low light Minimum signal reference ratio not met during air cali...

Page 33: ...error Plate air calibration data is unreasonable 504 NVRAM Carriage offset error Carriage offset data is unreasonable 505 NVRAM Stage offset error Stage offset data is unreasonable 506 NVRAM Battery T...

Page 34: ...ting our customers with the highest level of technical service Our Support website www moleculardevices com service support has a link to the Knowledge Base which contains technical notes software upg...

Page 35: ...optical performance Part Number Item Name Compatible Instruments 0200 6117 SpectraTest ABS1 Absorbance Validation Plate SpectraMax iD3 iD5 i3x i3 M2 M2e M3 M4 M5 M5e Plus 384 340PC 384 190 ABS ABS Plu...

Page 36: ...maximum Wavelength accuracy 1 0 nm across wavelength range Wavelength repeatability 0 2 nm Photometric range 0 to 4 000 OD Photometric resolution 0 001 OD Photometric accuracy linearity plate 0 2 0 OD...

Page 37: ...urement conditions endpoint column priority for dual wavelength measurements calibrate off 9 seconds single wavelength 19 seconds dual wavelength 425 650 nm 29 seconds single wavelength 59 seconds dua...

Page 38: ...n Read chamber Isothermal when temperature regulation is not enabled Range 4 C above ambient to 45 C when temperature regulation enabled The ambient temperature must be 20 C to achieve temperature reg...

Page 39: ...4 mm typical for 3 mL volume cells Test tubes 12 x 75 mm test tubes with test tube cover read in the cuvette chamber Compatibility SpectraMax Plus 384 Item Description Display 2 x 20 character back l...

Page 40: ...croplate Readers User Guide 40 0112 0126 B Item Description Size h x w x d 220 mm 8 6 x 580 mm 22 8 x 380 mm 15 Weight 13 6 kg 30 lb Power consumption 250 W Line voltage and frequency 90 250 VAC autor...

Page 41: ...tric range 0 to 4 000 OD Photometric resolution 0 001 OD Photometric accuracy linearity plate 0 2 0 OD 340 850 nm 1 0 and 0 006 OD Photometric precision repeatability 0 2 0 OD 340 850 nm 1 0 and 0 003...

Page 42: ...4 Item 96 wells 384 wells Endpoint standard read 9 seconds single wavelength 19 seconds dual wavelength 425 650 nm 29 seconds single wavelength 59 seconds dual wavelength 425 650 nm Kinetic read inter...

Page 43: ...Temperature regulation diagnostics Temperature regulation system is continuously monitored and updated Evaporation Plate lid required to minimize evaporative cooling Recommended plate Flat bottom pla...

Page 44: ...lel 25 pin to Centronics double shielding required Supported plates All 96 well and strip well plates including lids General Instrument SpectraMax 340PC384 Item Description Operating temperature 5 C t...

Page 45: ...OD 190 850 nm 1 0 and 0 003 OD Stray light 0 05 at 340 nm Photometric stabilization Instantaneous Photometric drift None continuous referencing of monochromatic input Calibration Automatic before eve...

Page 46: ...escription Read chamber Isothermal when temperature regulation is not enabled Range 4 C above ambient to 45 C when temperature regulation enabled The ambient temperature must be 20 C to achieve temper...

Page 47: ...trol panel 8 key membrane keypad Self diagnosis Continuous on board diagnostics Spill control Drawer mechanism and read chamber assembly protected from accidental spillage by drainage ports Computer i...

Page 48: ...Microplate Readers User Guide 48 0112 0126 B Item Description Size h x w x d 220 mm 8 6 x 580 mm 22 8 x 380 mm 15 Weight 13 6 kg 30 lb Power consumption 250 W Line voltage and frequency 90 250 VAC aut...

Page 49: ...1 0 and 0 006 OD Photometric precision repeatability 0 2 0 OD 340 850 nm 1 0 and 0 003 OD Stray light 0 05 at 340 nm Photometric stabilization Instantaneous Photometric drift None continuous referenci...

Page 50: ...Resolution 0 1 C Accuracy 1 0 C for plate chamber Temperature uniformity at equilibrium 0 5 C at 37 C Chamber warm up time 15 30 minutes measured on air after initiation of temperature regulation Tem...

Page 51: ...el 25 pin to Centronics double shielding required Supported plates All 96 well and strip well plates including lids General Instrument VersaMax Item Description Operating temperature 5 C to 40 C Opera...

Page 52: ...la norme NMB 001 du Canada ISM Equipment Classification Group 1 Class A This equipment is designated as scientific equipment for laboratory use that intentionally generate and or use conductively coup...

Page 53: ...5 cm Their fill volumes differ only because of their different internal width and chamber height dimensions Standard and Semi micro Cuvettes Several brands available Internal Width Minimum Volume Maxi...

Page 54: ...Standard Semi Micro Micro Hellma Cat No 100 104 105 004 104 002 108 002 105 Internal Dimensions 10 x 10 4 x 10 4 x 10 2 x 10 2 x 10 2 x 10 Fill Volume 4 mL 1 4 mL 600 L 700 L 500 L 300 L Ultra Micro C...

Page 55: ...y of Molecular Devices LLC or their respective owners Specifications subject to change without notice Patents www moleculardevices com productpatents FOR RESEARCH USE ONLY NOT FOR USE IN DIAGNOSTIC PR...

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