Chapter 1: Introduction
0112-0126 B
10
SpectraMax Plus 384 reads 96-well plates, 384-well plates and strip-well plates, and
cuvettes.
SpectraMax 190 reads 96-well and strip-well plates.
SpectraMax 340PC384 reads 96-well plates and 384-well plates and strip-well plates.
VersaMax reads 96-well and strip-well plates.
When you do reads at wavelengths below 340 nm, you must use special UV-transparent,
disposable or quartz plates to allow transmission of the deep UV spectra.
Not all manufacturers' plates are the same with regard to design, materials, or configuration.
Temperature uniformity within the plate may vary depending on the type of plate used.
CAUTION!
To prevent damage to the instrument, the height of the plate must not
exceed 25 mm, including the lid if the plate is lidded.
Shake
The software enables you to define settings to shake the plate linearly along the long axis at
preset intervals to mix of the contents of the wells.
Endpoint read type: Settings enable you to shake the plate for a definable number of
seconds and then read at all selected wavelengths.
Kinetic read type: Settings enable you to shake the plate for a definable number of
seconds before the initial read, and/or for a definable number of seconds before each
subsequent read.
Use of shake is recommended for ELISAs and other solid-phase, enzyme-mediated reactions
to enhance accuracy.
Cuvette Chamber
The cuvette chamber on the SpectraMax Plus 384 is located at the right front of the
instrument. You manually lift up the lid over the chamber to insert or remove a cuvette. The
chamber contains springs that position the cuvette in the proper alignment for a read. You
must manually close the cuvette door before you initiate a read.
Handle cuvettes on the frosted sides only. Place the cuvette into the chamber so that the
“reading” (clear) sides face left and right.
