Installing and Setting Up the SpectraMax MiniMax Imaging Cytometer
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Selecting the Light Source for Imaging
The SpectraMax MiniMax Imaging Cytometer captures images from the bottom of each
microplate well. You can choose to illuminate the sample with white transmitted light
from the top of the microplate using the Transmitted Light (TL) Detection Cartridge, or
use fluorescent excitation from the bottom of the microplate.
Item
Description
1
Transmitted Light (TL) Detection Cartridge
2
Path of white light from the Transmitted Light (TL) Detection Cartridge
3
Path of fluorescent excitation
4
Light source for fluorescent excitation
5
Camera lens
Figure 1-19: Path of Selected Light Sources
To illuminate the sample from the top, you need to have the Transmitted Light (TL)
Detection Cartridge installed in the detection cartridge drawer. See
(TL) Detection Cartridge on page 23
.
For best results with transmitted-light reads, use a microplate with no cover. A clear
cover can be used, if required. For fluorescent reads, you can use a solid cover on the
microplate.