Molecular Devices SpectraMax M2 User Manual Download Page 1

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0112-0102 E
October 2018

SpectraMax® M2
SpectraMax® M2e

Multi-Mode Microplate Readers

User Guide

Summary of Contents for SpectraMax M2

Page 1: ...0112 0102 E October 2018 SpectraMax M2 SpectraMax M2e Multi Mode Microplate Readers User Guide...

Page 2: ...and or license to use or permit others to use such manufacturers and or their product names as trademarks Each product is shipped with documentation stating specifications and other technical informa...

Page 3: ...tting Started 14 Control Panel 15 Cuvette Chamber 17 Plate Drawer 18 Plate Adapter 19 Chapter 4 Operating the Instrument 20 Temperature Settings 20 Wavelength Settings 21 Read a Plate 22 Read a Cuvett...

Page 4: ...User Guide 4 0112 0102 E Chapter 7 Troubleshooting 39 Opening the Drawer Manually 43 Obtaining Support 44 Appendix A Specifications 45 System Diagrams and Dimensions 51 Appendix B Accessories 53 Appe...

Page 5: ...ied by Molecular Devices the protection provided by the equipment might be impaired Warnings Cautions Notes and Tips All warning symbols are framed within a yellow triangle An exclamation mark is used...

Page 6: ...rective contact your dealer or local Molecular Devices office for the procedures to facilitate the proper collection treatment recovery recycling and safe disposal of the device California proposition...

Page 7: ...with the instructions outlined in this guide and take all the required precautions when using pathological toxic or radioactive materials Splashing of liquids can occur Take applicable safety precauti...

Page 8: ...strument Keep the instrument work area clear to prevent obstruction of the movement Provide clearance in front of the instrument of 18 cm 7 1 in for the plate drawer CAUTION To prevent damage to the i...

Page 9: ...Fluorescence instruments or instruments such as the SpectraMax M5 Multi Mode Microplate Reader or the SpectraMax iD5 Multi Mode Microplate Reader Read types include See Read Types on page 33 Endpoint...

Page 10: ...ata loss turn off all sleep and hibernation settings for the hard disk the CPU and the USB ports Disable automatic Windows updates Update Windows manually when you do not use the computer to control a...

Page 11: ...oint Kinetic and Spectrum read types are available for the Absorbance and Fluorescence read modes with cuvettes Note You can use a 12 x 75 mm test tube with a test tube cover You can order the test tu...

Page 12: ...ax Pro Data Acquisition and Analysis Software Installation Guide Unpacking the Instrument The packaging is designed to protect the instrument during shipment Tape is placed on the cuvette door to prot...

Page 13: ...at the power switch that is on the rear of the instrument is in the Off position To connect the instrument cables 1 Turn the instrument around so that the rear of the instrument faces you 2 Make sure...

Page 14: ...ment to complete its diagnostic check and the plate drawer opens The following are the main components of the instrument Control panel Enables you to open the plate drawer control the read chamber tem...

Page 15: ...ature inside the cuvette chamber The temperature inside the plate chamber displays in the software The center of the control panel displays the absorbance excitation and emission wavelengths the instr...

Page 16: ...eft buttons enable you to set the absorbance excitation fluorescence wavelength and the right buttons enable you to set the emission fluorescence wavelength Note The control panel does not display the...

Page 17: ...he instrument can accommodate standard height 45 mm 1 cm cuvettes and 12 x 75 mm test tubes when you use a test tube cover You can also use semi microcuvettes and ultra microcuvettes with an adapter S...

Page 18: ...The drawer closes for each read Subsequent plate drawer operation is dependent on the incubator setting When you open the drawer if the incubator is off the drawer remains open When you open the draw...

Page 19: ...res To insert the plate adapter 1 Open the plate drawer 2 Hold the plate adapter so that the label is on the front side facing up 3 Place the top back Row A of the plate adapter into the drawer first...

Page 20: ...ure from the Temperature Control dialog in the software When you turn off the incubator the chamber temperature gradually returns to ambient When you turn the incubator back on after a momentary shutd...

Page 21: ...on fluorescence wavelength and the right side wavelength buttons enable you to set the emission fluorescence wavelength To change the wavelength Press p or q once to increase or decrease the wavelengt...

Page 22: ...2 well 24 well or 48 well plates you must remove the plate adapter from the plate drawer See Plate Adapter on page 19 Before you place a plate in the drawer the underside of the plate must be dry If t...

Page 23: ...nstrument turn on the power switch The instrument carries out a diagnostic check During this check the plate drawer opens and then closes When the diagnostic check completes the plate drawer opens and...

Page 24: ...tity of light passing through a sample to a detector relative to the total quantity of light available Optical Density includes absorbance of the sample plus light scatter from turbidity and backgroun...

Page 25: ...re to normalize the data to a 1 cm pathlength PathCheck Pathlength Measurement Technology The temperature independent PathCheck Pathlength Measurement Technology normalizes your absorbance values to a...

Page 26: ...ter or buffer to use the Cuvette Reference correction method typically not necessary when you use aqueous solutions with minimal alcohol salt or organic solvent content Water Constant The PathCheck te...

Page 27: ...not apply PathCheck technology to the absorbance values If you do select to use PathCheck technology for the plate read you cannot apply the PathCheck Pathlength Measurement Technology feature after t...

Page 28: ...liably correct for turbidity Samples that are highly colored in the upper visible spectrum might have absorbance that extends into the near infrared NIR spectrum and can interfere with the PathCheck t...

Page 29: ...rials have well characterized excitation and emission spectra The following figure shows an example of excitation and emission spectra for a fluorophore The excitation and emission bands are each fair...

Page 30: ...gure shows that the best results are often obtained when the excitation and emission wavelengths you use for the read are not the same as the peak wavelengths of the excitation and emission spectra of...

Page 31: ...not flash so no background interference occurs A dark estimate is done over a dark reference and multiple reads are averaged together into one read per well The default setting for the Luminescence r...

Page 32: ...ating electronics introduce a delay between the cutoff of each light pulse and the start of signal collection During the delay the unspecific fluorescence caused by test compounds assay reagents and t...

Page 33: ...F reagent kits If you do not use a kit then start with a delay of 50 s and try different delays up to 400 s with a fixed integration time of 400 s After you select the optimum delay based on the highe...

Page 34: ...dpoint analysis Spectrum The Spectrum read type measures optical density OD or Transmittance across a specified wavelength range with allowed values from 200 nm to 1000 nm For the Fluorescence or Lumi...

Page 35: ...o If parts are returned they must be enclosed in a sealed plastic bag that states that the contents are safe to handle and are not contaminated WARNING BIOHAZARD Always wear gloves when operating the...

Page 36: ...rument requires periodic cleaning The frequency of cleaning depends on the cleanliness of the lab and could range from once a month to once every six months To clean the fan filter 1 Remove the plate...

Page 37: ...he main power source before you do a maintenance procedure that requires removal of a panel or cover or disassembly of an interior instrument component 1 Power off the instrument 2 Unplug the power co...

Page 38: ...ment in the original packaging materials Correctly repacking the instrument includes following applicable decontamination procedures and packing instructions CAUTION When transporting the instrument w...

Page 39: ...istance of Technical Support 500 599 Errors due to failure or improper initialization of the instruments non volatile memory NVRAM All of these errors require the assistance of Technical Support Table...

Page 40: ...ument could not perform the give command because it was busy doing another task 106 command invalid measurement in progress Instrument could not perform command because a measurement was in progress 1...

Page 41: ...rial port parity error Parity bit error detected with incoming serial data 204 serial port overrun error Caused by host computer sending too much data and ignoring the flow control signal 205 serial p...

Page 42: ...door being open 305 reference level saturation During a cuvette read could be due to cuvette door being open 306 plate air cal fail low light Minimum signal reference ratio not met during air calibrat...

Page 43: ...air calibration data is unreasonable 504 NVRAM Carriage offset error Carriage offset data is unreasonable 505 NVRAM Stage offset error Stage offset data is unreasonable Table 7 6 Error Codes 500 599 N...

Page 44: ...ers with the highest level of technical service Our Support website www moleculardevices com service support has a link to the Knowledge Base which contains technical notes software upgrades safety da...

Page 45: ...ance measurements agreement with cuvette absorbance measurements for the same solution requires that the solution volume in the plate well is between 100 L and 300 L Note Technical specifications are...

Page 46: ...ometric precision repeatability 1 0 and 0 003 OD Stray light 0 05 at 230 nm Photometric stabilization Instantaneous Photometric drift None continuous referencing of monochromatic input Calibration Aut...

Page 47: ...15 pM fluorescein Excitation wavelength range 250 850 nm Emission wavelength range 360 850 nm M2 250 850 nm M2e Wavelength selection Monochromators tunable in 1 nm increments Bandwidth excitation emis...

Page 48: ...ngth range 250 850 nm Crosstalk 0 5 in 96 and 384 well microplates Table A 4 Luminescence Photometric Performance Item Description Front Panel Operation Single wavelength Absorbance Transmittance Fluo...

Page 49: ...ead with PathCheck Pathlength Measurement Technology 96 wells in 2 07 minutes single wavelength absorbance 384 wells in 7 19 minutes single wavelength absorbance Microplate read time endpoint Speed re...

Page 50: ...must be 20 C Resolution Resolution 0 1 C Accuracy 1 0 C for microplate and cuvette chamber Temperature uniformity at equilibrium 0 5 C at 37 C Chamber warmup time 15 30 minutes measured on air after...

Page 51: ...Appendix A Specifications 0112 0102 E 51 System Diagrams and Dimensions In the following drawings the dimensions are show in centimeters and inches Front View with Dimensions Side View with Dimensions...

Page 52: ...SpectraMax M2 and M2e Microplate Reader User Guide 52 0112 0102 E Top View with Dimensions...

Page 53: ...6 Falcon The software plate list also includes half area and low volume plates Note Not all manufacturers plates and cuvettes are the same with regard to design materials or configuration Depending o...

Page 54: ...rdware as well as absorbance fluorescence and luminescence detection test tools with its SpectraTest solutions The SpectraTest line of microplate reader validation packages provide automated and compr...

Page 55: ...me Delay 4601 0014 Test Tube Cover 2300 0277 Power cord USA Canada 4400 0002 Power cord Continental Europe C13 4400 0036 Power cord United Kingdom Ireland C13 4400 0037 Power cord Australia C13 4400 0...

Page 56: ...quipment Classification Group 1 Class A This equipment is designated as scientific equipment for laboratory use that intentionally generate and or use conductively coupled radio frequency energy for i...

Page 57: ...y of Molecular Devices LLC or their respective owners Specifications subject to change without notice Patents www moleculardevices com productpatents FOR RESEARCH USE ONLY NOT FOR USE IN DIAGNOSTIC PR...

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