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Chapter 1: Introduction
The SpectraMax® M2 Multi-Mode Microplate Reader and the SpectraMax® M2e Multi-Mode
Microplate Reader are dual monochromator, multi-detection instruments with a triple-mode
cuvette port and a 6-well to 384-well plate read capability.
The flexibility and high sensitivity makes these instruments appropriate for applications in
the fields of biochemistry, cell biology, immunology, molecular biology, and microbiology.
Typical applications include ELISA, nucleic acid, protein, enzymatic type homogeneous and
heterogeneous assays, microbial growth, endotoxin testing, and pipettor calibration.
Read Mode
SpectraMax M2
SpectraMax M2e
Absorbance
Fluorescence Intensity
(Top read only)
(Top and bottom read)
*Luminescence
(Secondary Read Mode)
(Secondary Read Mode)
*Time-Resolved Fluorescence
(Secondary Read Mode)
(Secondary Read Mode)
Read Modes
*Use secondary read modes for limited development of glow Luminescence or Time-Resolved
Fluorescence assays. The performance of these modes is not comparable to dedicated Luminescence
or Time-Resolved Fluorescence instruments or instruments such as the SpectraMax® M5 Multi-Mode
Microplate Reader or the SpectraMax® iD5 Multi-Mode Microplate Reader.
Read types include: See
Endpoint
Kinetic
Spectrum
Well Scan
The instrument light source is a high-powered Xenon flash lamp. Two holographic diffraction
grating monochromators allow for individual optimization of wavelengths for both excitation
and emission in the Fluorescence read mode. Mirrored optics focus the light in to the sample
volume, and cutoff filters reduce stray light and minimize background interference. You can
vary the number of lamp flashes per read to optimize the sensitivity or read speed.
The instrument dynamic range of detection is from 10
-6
to 10
-12
molar fluorescein. Internal
compensation for detector sensitivity, photomultiplier tube voltage and sensitivity, as well
as excitation intensity, effectively eliminates variations in measured fluorescence values. The
photometric range is 0–4 ODs with a resolution of 0.001 OD.
Dual monochromators enable you to select absorbance wavelengths between 200 nm and
1000 nm, excitation wavelengths between 250 nm and 850 nm, and emission wavelength
between:
360 nm and 850 nm (SpectraMax M2).
250 nm and 850 nm (SpectraMax M2e).
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