SpectraMax M2 and M2e Microplate Reader User Guide
23
0112-0102 E
5. Place the plate onto the plate drawer, matching well A1 with position A1 in the drawer.
Note the following:
If you read 6, 12, 24, or 48-well plates, make sure that the plate is flat against the
drawer bottom.
If you do a top read for 96 or 386-well plates, make sure that the plate is flat against
the plate adapter.
6. Do one of the following in the software:
Open a data file or protocol file that contains the experiment settings for the read.
Create a new protocol file with the settings for the read.
7. In the software, in the Ribbon, on the Home tab, click
Read
to read the plate.
After the read completes, the plate drawer opens and you can remove the plate.
Note:
If the incubator is on, the drawer closes after approximately 10 seconds. If you
return to the instrument after a read and the plate drawer closed, press
Drawer
to
open the drawer and remove the plate.
Read a Cuvette
Handle a cuvette on the frosted sides only.
1. On the rear of the instrument, turn on the power switch.
The instrument carries out a diagnostic check. During this check, the plate drawer opens,
and then closes. When the diagnostic check completes, the plate drawer opens and
remains open.
2. Power on the computer.
3. Start the SoftMax Pro Software and connect the software to the instrument.
4. In the software, in the Ribbon, on the Home tab, click
Acquisition Settings
to display
the Settings dialog where you define the acquisition settings for the Cuvette Set section.
5. Place the cuvette in the chamber so that the read (clear) sides face left and right. Make
sure that the cuvette is completely seated in the chamber. (Springs hold the cuvette in
place.)
6. Close the cuvette chamber door.
7. Do one of the following:
If the cuvette is a reference cuvette, press
REF
to acquire the reference read of either
buffer, water, or air.
The instrument calibrates in less than two seconds, closes the plate drawer if it is
open, and then reads the cuvette.
If the cuvette is a sample cuvette, to acquire the sample read, press
Read Cuvette
.
The plate drawer closes if it is open, and the instrument reads the cuvette according
to the software settings.
8. After the read, remove the cuvette from the chamber.
