Molecular Devices SpectraMax 190, Operator'S Manual

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SPECTRAmax
190
Microplate Spectrophotometer Operator’s Manual vii
Conventions
Used in this
Manual
The names of keys that appear on the SPECTRAmax 190 control panel are
shown in boxed Helvetica type. Example:
[Drawer]
.
Italic and boldface type are used for emphasis. Examples: “Press carefully to
engage,” “Do not press down.”
A note provides information that will help you properly execute an
action or procedure.
Indicates an action or condition that could potentially dam-
age the instrument or one of its components or could result in loss of
data.
Indicates a situation that could result in potential injury to a
person working with the system.
Indicates a condition involving potentially infectious bio-
logical agents requiring that proper handling precautions be taken.
Glossary of
Terms
Absorbance, A
The amount of light absorbed by a solution. To measure absorbance accu-
rately, it is necessary to eliminate light scatter. In the absence of turbidity,
absorbance = optical density.
A = log (I
0
/I)
I
0
= incident light
I = transmitted light
In this manual, we use the terms absorbance and optical density
interchangeably.
Optical Density, OD
The amount of light passing through a sample to a detector relative to the
total amount of light available. Optical Density includes absorbance of the
sample plus light scatter from turbidity.
Transmittance, T
The ratio of transmitted light to the incident light.
T = I/I
0
%T = 100 T
NOTE:
!
CAUTION:
WARNING:
BIOHAZARD: