Molecular Devices SpectraMax 190, Operator'S Manual

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Chapter 1
1-12 SPECTRAmax
190 Microplate Spectrophotometer Operator’s Manual
Speci Þ cations
Thermal speci Þ cations for microplates used in the SPECTRAmax 190 apply to
ß at-bottom microplates with isolated wells. All other microplate speci Þ ca-
tions apply to standard 96-well polystyrene ß at-bottom microplates.
Technical speci Þ cations are subject to change without notice.
Photometric Performance
Wavelength range 190–850 nm
Wavelength selection Monochromator tunable in 1-nm increments
Wavelength bandwidth
≤
2.0 nm FWHM (full width half maximum)
Wavelength accuracy ± 1.0 nm across wavelength range
Wavelength repeatability ± 0.2 nm across all optical channels
Photometric range -0.3 to 4.000 OD
Photometric resolution 0.001 OD
Photometric accuracy/linearity
(microplate) , 0 – 2.0 OD: 190–850 nm <± 1.0% and ± 0.006 OD
Photometric precision
(repeatability) , 0 – 2.0 OD: 190–850 nm <± 1.0% and ± 0.003 OD
Stray light
≤
0.05% at 340 nm
Photometric stabilization Instantaneous
Photometric drift None—continuous referencing of monochromatic output
Calibration Automatic before first kinetic read and before every end-
point reading
Optical alignment None required
Light source Xenon flash lamp (5 watts)
Average lamp lifetime 1 billion flashes
Illumination Top down
Photodetectors Silicon photodiode
Photometric Analysis Modes
Stand-Alone
• Single wavelength Absorbance reading of the microplate
Using SOFTmax PRO
• Express data as Absorbance or %Transmittance
• Single wavelength reading of microplate
• Multiple wavelength (up to six) reading of microplate
• Kinetic and kinetic graphics of microplate
• Spectral scan (190–850 nm) of microplate
Measurement Time (calibration off)
NOTE: