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SkanWasher 400 User Guide

E-5

SkanWasher 400 Wash Program Sheet

Instrument Parameters

Wash Sequence

Program Number

Program Name

Date

Signature

Description

Air Pressure

[bar] Volume Adjust

σ

V

[-]

Aspirate Probe

[mm] Dispense Probe

[mm]

1.

Wash Step

Aspirate Wash Dispense Soak Rinse _____ sec/____ µl

2.

Wash Step

Aspirate Wash Dispense Soak Rinse _____ sec/____ µl

3.

Wash Step

Aspirate Wash Dispense Soak Rinse _____ sec/____ µl

4.

Wash Step

Aspirate Wash Dispense Soak Rinse _____ sec/____ µl

5.

Wash Step

Aspirate Wash Dispense Soak Rinse _____ sec/____ µl

6.

Wash Step

Aspirate Wash Dispense Soak Rinse _____ sec/____ µl

7.

Wash Step

Aspirate Wash Dispense Soak Rinse _____ sec/____ µl

8.

Wash Step

Aspirate Wash Dispense Soak Rinse _____ sec/____ µl

9.

Wash Step

Aspirate Wash Dispense Soak Rinse _____ sec/____ µl

10. Wash Step

Aspirate Wash Dispense Soak Rinse _____ sec/____ µl

11. Wash Step

Aspirate Wash Dispense Soak Rinse _____ sec/____ µl

12. Wash Step

Aspirate Wash Dispense Soak Rinse _____ sec/____ µl

13. Wash Step

Aspirate Wash Dispense Soak Rinse _____ sec/____ µl

14. Wash Step

Aspirate Wash Dispense Soak Rinse _____ sec/____ µl

15. Wash Step

Aspirate Wash Dispense Soak Rinse _____ sec/____ µl

16. Wash Step

Aspirate Wash Dispense Soak Rinse _____ sec/____ µl

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Summary of Contents for SkanWasher 400

Page 1: ...SkanWasher 400 User Guide AN ALL INCLUSIVE AUTOMATIC MICROPLATE WASHER...

Page 2: ...nd is not to be used in any type of promotion or advertising without permission from MDC All other trademarks or registered trademarks are the property of their respective owners Disclaimer Molecular...

Page 3: ...Rear Connections 11 Internal Washer Parts 13 Internal Vacuum Source 13 Internal Pressure Source 13 Chapter 4 Software Configuration 15 Setup Function 16 Edit Program 16 Edit Options 20 Chapter 5 Oper...

Page 4: ...zontal Adjustment 34 Error Messages 35 Appendix A RS 232 Operation A 1 Introduction A 1 Parameters A 1 Communication Protocol A 1 Appendix B Shipment B 1 Appendix C Specifications C 1 Physical C 1 Per...

Page 5: ...croplate holder and lift 8 Figure 5 SkanWasher 400 9 Figure 6 Control panel LCD and touch keys 10 Figure 7 Rear of the SkanWasher 400 11 Figure 8 Aspirate probe height position 18 Figure 9 Dispense pr...

Page 6: ...vi SkanWasher 400 User Guide Table 1 Wash Step Selections 19 Table 2 Troubleshooting Chart 33 Table A 1 Character Commands Recognized A 2 Table A 2 Character Replies Sent A 3 Tables...

Page 7: ...ing is an essential component of the assay The SkanWasher 400 is designed to wash 96 well microplates The process of setting up wash parameters is flexible and allows you to modify or choose the follo...

Page 8: ...6 Detailed procedures will be listed for care and maintenance of instrument Chapter 7 Troubleshooting tips error messages and advanced troubleshoot ing will be outlined Appendix This section will incl...

Page 9: ...e reservoir Sensors can be disabled through the Setup menu Edit Options Consult page 20 for procedure Serial Number The serial number of the unit can be found on the back panel of the instrument Packa...

Page 10: ......

Page 11: ...of the instrument Plug the power cord into the main power outlet Step 4 Connect Waste Reservoir refer to Figure 1 Connect the outlet tubing line red from the waste reservoir to the Outlet port on the...

Page 12: ...reservoir to the Pressure port on the back of the instrument The large connector at the end of the tubing will be used for emptying of the head and will not be connected during normal operation CAUTIO...

Page 13: ...the instrument Figure 2 Wash and rinse liquid reservoirs IMPORTANT Only one reservoir bottle can be connected at one time for either wash buffer or rinse solution since the unit has only one inlet por...

Page 14: ...type round or flat bottomed wells in use by selecting the correct printed pattern on the side of the microplate holder See Figure 3 Push the plate holder onto the two rods on the microplate lift see...

Page 15: ...of the wash program The vertical velocity of the lift is fixed except during the aspirate function the longer the aspirate time the slower the lift moves upward toward the aspirate probes This creates...

Page 16: ...s Setup Program Enter and the up and down arrow keys are used to select and enter parameters when programming the SkanWasher 400 When the Setup or Program key is pressed its corresponding LED will lig...

Page 17: ...re three LEDs that light up indicating various functions Setup LED will light when user is in setup mode Program LED will light when user is in program mode Ready LED will light when unit is ready for...

Page 18: ...Located on the back panel of the unit This contains the main power receptacle which includes the power switch and fuses A voltage switch located below the power cord receptacle allows the user to set...

Page 19: ...iminating the need for gravity feed The pressure level can be adjusted between 0 0 and 0 6 bar approximately 9 psi The air pressure is supplied through the small quick couplings on the rear side of th...

Page 20: ......

Page 21: ...robe above the bottom of the well during aspiration can be varied The amount of fluid kept in the well can be adjusted Dispense probe height position in the well during washing can be set Up to 16 was...

Page 22: ...n pressing the Setup key the Setup Main menu will display two options Edit Program A wash program can be edited or created using this selection Edit Options Instrument parameters can be viewed and sen...

Page 23: ...o adjust the inlet wash liquid flow To disable the pressure pump select 0 0 bar For normal wash select 0 25 bar approximately 4 psi Volume Adjust Adjustment can be made to fine tune the dispensed vol...

Page 24: ...icroplate in lift and press Enter to raise microplate to probes Use up down arrow buttons to set at correct height See Figure 9 Total adjustable distance is 6 mm Wash Program 1 16 Up to 16 wash steps...

Page 25: ...t volume of wash from 100 900 l Wash time Set time from 0 1 to 10 seconds Aspirate Set aspiration time from 2 0 10 seconds Lift velocity is varied with selected aspirate time The lift velocity is auto...

Page 26: ...r On Off Read Sensor Sensor On Off The sensors can be enabled or disabled by selecting this option Step 1 Press Setup on the Control Panel to enter the Setup Main Menu Step 2 Press Down arrow key to s...

Page 27: ...T When sensors disabled all three safety features will be disengaged Air Pressure Value is actual air pressure on internal pressure source displayed in bar Changes can be made through software program...

Page 28: ......

Page 29: ...lder Check pattern on the side of microplate holder to verify that the holder is in the correct plate configuration Press Rinse key to enter prime program Press Start Repeat 3 times Step 5 Choose Wash...

Page 30: ...late Holder Check pattern on the side of microplate holder to verify that the holder is in the correct plate configuration Step 7 Press Start key Step 8 Repeat Step 5 8 to wash additional plates Step...

Page 31: ...r Step 3 Verify waste tubing is connected to outlet port Step 4 Insert a clean microplate into the microplate holder Step 5 Press the Rinse key to enter the Rinse Prime mode Step 6 Press the Rinse key...

Page 32: ...p 2 Disconnect liquid line from the Inlet port on the instrument s wash head Step 3 Connect large connector from pressure tubing to Inlet port Step 4 Verify waste tubing is connected to outlet port St...

Page 33: ...ogram will rinse for 5 seconds and then soak the probe tips for 5 seconds This cycle is repeated 3 times Step 6 Soak Probes Press Stop key to pause when instrument is in first soak cycle of Rinse prog...

Page 34: ...cles Press the Rinse key to enter the Rinse Prime mode Press the Start key a second time to start the prime program Repeat 3 times Step 7 Run a wash program to verify that particles are removed Step 8...

Page 35: ...se Prime mode Press the Start key a second time to start the prime program Water will flush out through the holes in the back of the wash head Repeat if indicated Step 7 Reseat inlet channel seal bloc...

Page 36: ...et access to the wash head outlet channel see Figure 11 Remove two hex wrench screws using the hex wrenches available in the tool kit Pull out the wash head block to expose the outlet channels Step 2...

Page 37: ...Loctite Threadlocker Green does not set up as quickly as QuickTite Microplate Lift Adjustment Tilt and Vertical The microplate lift is factory adjusted for optimal operation and should nor mally not n...

Page 38: ...Tilt Adjustment Screw to tilt the placeholder upward loosen this screw to tilt the plateholder down Figure 12 Microplate lift adjustment CAUTION Don t use force to tilt the plate holder If you need t...

Page 39: ...o power to instrument instrument will not turn on Check that power cord is plugged in Check power active to source outlet Check voltage selection switch Check power supply Air in lines Prime lines inc...

Page 40: ...djust Programs not retained in instrument memory Battery on main board may be low battery life is from three to five years Test the battery strength to see if it needs to be replaced Strength should b...

Page 41: ...e prime The actual air pressure is not equal to the set air pressure value Low Probably a leak Check that all reservoir caps are tightened High Due to changed air pressure setting The previ ously used...

Page 42: ......

Page 43: ...arity None Stop bits 1 The SkanWasher 400 comes with an RS 232 cable having 9 pin male female connectors The communication protocol is designed for single character commands No data handshake control...

Page 44: ...ished Reply G 47h E 45h 21h S s 57h 73h Stop interrupt the current action of the Skan Washer This command performs the same function as pressing the Stop key on the control panel The Stop command must...

Page 45: ...ram that was not defined The SkanWasher 400 will only accept a select program com mand if the wash program number between 1 and 8 has been defined 1 31h 2 32h 8 38h The wash program now selected 3Fh C...

Page 46: ...a microplate is present will be verified prior to initiating the wash Start command accepted Reply G Not accepted due to sensor alarm Reply E When wash sequence finished Reply S s 57h 73h Stop interr...

Page 47: ...ls are used use appropriate decontami nate solution for each material Step 2 Perform Empty Head procedure to empty dispense head of all fluid Step 3 Clean exterior surface of instrument with alcohol f...

Page 48: ......

Page 49: ...brane pump Microplate lift Step motor driven Performance Aspirate 2 l average residual volume well Dispense Precision 2 CV Accuracy 3 Internal volume Wash head prime volume 35 ml Well flow rate water...

Page 50: ......

Page 51: ...umber ____________________ Method of Decontamination ______________________________________ _____________________________________________________________ ______________________________________________...

Page 52: ......

Page 53: ...Rinse 2 0 sec ____ l 4 Wash Step Aspirate Wash Dispense Soak Rinse 1 5 sec ____ l 5 Wash Step Aspirate Wash Dispense Soak Rinse 2 0 sec ____ l 6 Wash Step Aspirate Wash Dispense Soak Rinse 1 5 sec __...

Page 54: ...2 0 sec ____ l 4 Wash Step Aspirate Wash Dispense Soak Rinse _____ sec 250 l 5 Wash Step Aspirate Wash Dispense Soak Rinse 2 0 sec ____ l 6 Wash Step Aspirate Wash Dispense Soak Rinse _____ sec 250 l...

Page 55: ...p Aspirate Wash Dispense Soak Rinse 2 0 sec ____ l 4 Wash Step Aspirate Wash Dispense Soak Rinse _____ sec 200 l 5 Wash Step Aspirate Wash Dispense Soak Rinse 5 0 sec ____ l 6 Wash Step Aspirate Wash...

Page 56: ...ate Wash Dispense Soak Rinse _____ sec ____ l 4 Wash Step Aspirate Wash Dispense Soak Rinse _____ sec ____ l 5 Wash Step Aspirate Wash Dispense Soak Rinse _____ sec ____ l 6 Wash Step Aspirate Wash Di...

Page 57: ...se _____ sec ____ l 6 Wash Step Aspirate Wash Dispense Soak Rinse _____ sec ____ l 7 Wash Step Aspirate Wash Dispense Soak Rinse _____ sec ____ l 8 Wash Step Aspirate Wash Dispense Soak Rinse _____ se...

Page 58: ...se _____ sec ____ l 6 Wash Step Aspirate Wash Dispense Soak Rinse _____ sec ____ l 7 Wash Step Aspirate Wash Dispense Soak Rinse _____ sec ____ l 8 Wash Step Aspirate Wash Dispense Soak Rinse _____ se...

Page 59: ...vices Corporation at the telephone number or address below to locate the nearest service facility At the time of requesting warranty service you must present proof of purchase documentation which incl...

Page 60: ...this agreement is for the sole purpose of identifying them Any such description is not part of the basis of the bargain and does not constitute a warranty that the products shall conform to that desc...

Page 61: ...5 27 B 1 D 1 Dispense Probe Position 18 Dispense Volume 19 E Edit Options 16 20 Edit Program 16 Empty Wash Head Procedure 26 Enter key 10 Error messages 35 H Home position microplate lift 9 I Installa...

Page 62: ...al number 3 Set Aspirate Position 17 Set Dispense Position 18 Setup function 16 Setup key 10 Setup LED 11 Shipment B 1 Software 15 Specifications C 1 Start key 10 Stop key 10 T Tilt adjustment screw 3...

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