Miltenyi Biotec UltraMicroscope II, User Manual

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If it is not possible to align the light sheet by lowering or raising the alignment tool, the actual position of
the horizontal focus does not match with the position displayed in the ImSpector software. Align the
central light sheet as well as possible before adjusting the horizontal focus.
Adjusting the horizontal focus
1 Select the Advanced tab on the bottom of the Settings 1 window.
2 Move the slider Sheetmotor Calibration until only one line passes the pinhole. Ensure that the light sheet
focus is always in the center and the Optics > sheet NA is at the maximum. If necessary, readjust the z-
direction.
3 If only one line passes the pinhole in the center of the mask, activate the intensity profile.
4 Increase the intensity of this light sheet by switching between moving the alignment tool in z-direction and
moving the slider Sheetmotor Calibration . If not any further increase of the intensity is measurable, the
central light sheet is aligned.
5 Click Save Settings in the toolbar to fix the adjusted horizontal focus.
6 Click Set as Zero under Current Position to set a reference point of the alignment tool.