Miltenyi Biotec UltraMicroscope II, User Manual

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Introduction
2.1 The UltraMicroscope II Imaging System
Understanding spatially complex biological systems, like the vasculature of an entire organ, requires the
availability of high-resolution information on their tissue architecture. The light sheet microscopy can provide
this information.
To visualize larger samples with the same z-resolution, the UltraMicroscope II Imaging System uses dynamic
horizontal focusing. Samples remain stationary while the focus position moves, shifting the narrow range of
detection horizontally to generate a series of snapshots. These snapshots are blended into one high-quality
image by the software.
The instrument uses two opposing sets of three light sheets, each positioned at a slightly different angle. All
six light sheets converge on the focal plane to illuminate all areas of the sample and minimize shadow artifacts.
Improved optional sectioning gets the most out of the sample.
The UltraMicroscope II Imaging System can be equipped with one of the following modules:
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the Zoom Body Module for easy handling in a multi-user environment
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the Super Plan Module that can deliver unprecedented image resolution and image quality, and an automated
magnification changer
The Super Plan Module can be mounted directly to the focusing unit of existing systems.
The UltraMicroscope II Imaging System can be upgraded with an in vivo sample cuvette, which allows setting
and maintaining of a constant immersion medium temperature and CO₂/O₂ atmosphere within the cuvette.
2.2 Intended use
The UltraMicroscope II Imaging System is a semiautomated light sheet microscope for imaging large or
multiple samples. The sample may only be examined in the supplied cuvette. Unauthorized modifications of
the cuvette or the instrument as a whole are not permitted for safety reasons. The cuvette may only be filled
with MACS Imaging Solution or another imaging solution approved by Miltenyi Biotec. The instructions for
operation, maintenance, and service described in this user manual must be observed.
The UltraMicroscope II Imaging System is for research use only in a laboratory environment. Only laboratory
professional users may use the instrument.