Miltenyi Biotec UltraMicroscope II, User Manual

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Laser alignment
6.1 Light sheet alignment
To generate high-contrast images with the best z-resolutions, all light sheets must be perfectly aligned to the
same z-plane. A set of adjustable precision screws on the left-hand and the right-hand side of the instrument
can be used to align the individual beams creating the light sheet. The Z screws enable an adjustment of the
light sheets in terms of the z-plane. Screw Z1 is used for aligning the central light sheet on the left-hand side in
the z-plane. Later the screw can be used for the fine adjustment of the z-shift between the left and right. The
screws Z2 and Z4 are used for aligning the outer light sheets. The x-ed out screws may not be adjusted. Always
start with the central light sheet before aligning the lateral light sheets.
Figure 6.1: Side view of the instrument with the positions of the adjustable precision screws
Alignment tool
The alignment tool facilitates the adjustment of all light sheets. The mask carries an X with a pinhole in the
middle. All light sheets must pass the pinhole.
Figure 6.2: Side view of the alignment tool