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00110230w  Rev 10/20 

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Introduction

The Scepter™ 3.0 Handheld Automated Cell Counter 

(“Scepter™ 3.0”) provides a fast and convenient method 

for counting cells or particles using a disposable sensor. 

Using the Coulter Principle, the Scepter™ 3.0 does all the 

work and rapidly delivers accurate and reliable cell counts.

The Scepter™ 3.0 quantifies cells based on size and 

discriminates larger cells from smaller debris, unlike 

vision-based techniques, which rely on object recognition 

software and cannot reliably detect small cells.  

A sample of interest is diluted, then acquired using 

a precision-engineered, microfluidic Scepter™ 3.0 

disposable sensor. In seconds, Scepter™ 3.0 displays cell 

concentration, cell diameter or volume measurements, 

along with a histogram of cell distribution. 

Up to 999 histograms can be stored in the instrument. 

The Scepter™ 3.0 has a wireless network interface which 

allows you to send histograms and data files to your  

Wi-Fi

®

 printer or computer easily. No more cables.

The new Charging Station stores Scepter™ 3.0 out-of-the-

way when not in use,  

freeing up valuable hood or lab bench space.  

This system is intended for research use only.

Contents

Introduction  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  . 2

Symbols and Safety Precautions   .  .  .  . 3

Parts and Functions   .  .  .  .  .  .  .  .  .  .  .  .  .  . 4

Features 4

Control Buttons and Screen Icons  

4

Battery 5

Installing the Charging Station 

6

Getting Started  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  . 7

Date and Time 

7

Bluetooth

®

 Enabled Printer 

7

Wi-Fi

®

 Network 

8

Gating Method 

10

Display Units 

10

Sensors    .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  . 11

Technology 11

Attach the Sensor 

11

Cell Counting   .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  . 12

Cell, Bead, and Particle Suspensions  12

Scepter™ 3.0 Test Beads 

12

Counting Process 

13

Histogram Explorer   .  .  .  .  .  .  .  .  .  .  .  .  . 13

Go to Home  

13

Edit Analysis  

14

Switch to Volume / Diameter 

14

Scale Y-Axis Count 

14

Exporting Data 

15

Reviewing Saved Data 

16

Deleting Saved Data 

16

Printing    .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  . 17

Help Screens .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  . 17

Tutorials 17

DNS, IP, Mac

®

 Address  

17

Firmware Updates   .  .  .  .  .  .  .  .  .  .  .  .  .  . 17

Storage and Maintenance .  .  .  .  .  .  .  .  . 18

Storage 18

Cleaning/Sanitizing 18

Maintenance 18

Troubleshooting  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  . 18

Error Messages 

19

Notice 21

Specifications    .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  . 22

Contact Information 

22

Technical Assistance 

22

Standard Warranty 

22

Product Ordering   .  .  .  .  .  .  .  .  .  .  .  .  .  .  . 23

Global Registration  

Marks of Conformity  .  .  .  .  .  .  .  .  .  .  .  .  . 24

Summary of Contents for PHCC30000

Page 1: ...perates as MilliporeSigma in the U S and Canada The life science business of Merck KGaA Darmstadt Germany operates as MilliporeSigma in the US and Canada SigmaAldrich com User Guide Scepter 3 0 Handhe...

Page 2: ...r computer easily No more cables The new Charging Station stores Scepter 3 0 out of the way when not in use freeing up valuable hood or lab bench space This system is intended for research use only Co...

Page 3: ...nstrument to vibrations Vibrations may cause instrument malfunction or damage Do not autoclave or expose to high temperature To avoid damage to internal electronic components do not spray instrument w...

Page 4: ...s the sensor Tech Service Port For Service use only USB Port Connects instrument to USB thumb drive Ergonomic Hook Allows comfortable handling of the instrument Secures the instrument to charger Contr...

Page 5: ...the Recharging screen when placed in the Charging Station the following steps must be followed to start charging the Scepter 3 0 battery 1 Place Scepter 3 0 into the Charging Station 2 While on the s...

Page 6: ...kable surface 1 Use double sided mounting tape 2 Remove tape liner from one side of tape and affix to back of mounting plate as shown below 3 Remove remaining tape liner and press firmly onto glass me...

Page 7: ...14 16 Edit Time Hours Minutes Seconds 14 16 34 Press OK to accept 01 Feb 18 14 16 Edit Time Hours Minutes Seconds 14 16 34 Press OK to accept 1 Scroll to Edit Time and press the OK button 2 Scroll to...

Page 8: ...nd select Properties If the File system is identified as FAT32 no formatting is needed Continue to Creating a Wi Fi Configuration File NOTE This view will also show how much space is available on your...

Page 9: ...file from the computer onto the USB thumb drive Once the file has loaded remove the USB thumb drive from the computer port Connect to Wi Fi Network 1 From the Main Menu select Settings and press the...

Page 10: ...were last set by the user either during a count or during post count data management 01 Feb 18 14 16 Menu Home Histograms Settings Help 01 Feb 18 14 16 Settings Back Date Time Bluetooth Wifi Gating M...

Page 11: ...epter 3 0 instrument Do not use sensor if visibly damaged Attach the Sensor 1 When the Scepter 3 0 is powered on it will show Attach the Sensor to begin 2 Holding the sensor with the sensor size numbe...

Page 12: ...net is recommend use low vacuum 5 Hg Cell Bead and Particle Suspensions Materials Required Cell sample Scepter 3 0 Test Beads Cat No PHCC3BEADS or particle suspensions Note Some particle suspensions...

Page 13: ...ion and diameter or volume will be displayed on the screen Press the OK button to save up to 999 histograms in the Scepter 3 0 memory and open the Histogram Explorer Press the OK button to return to H...

Page 14: ...03 Dec 17 Count 0 900 36 24 30 18 12 6 Dia in m 3 342e5 15 685 mL m Conc Dia 01 Feb 18 14 16 Histogram Edit Gates High gate Done 26 575 Low gate 12 585 Count 0 900 36 24 30 18 12 6 Dia in m Switch to...

Page 15: ...wirelessly Materials Required Wireless Network broadcasting at 2 4 Ghz PC Mac or UNIX Computer connected to the same Wi Fi Network as Scepter 3 0 Scepter 3 0 Instrument Follow Wi Fi Network p 8 direct...

Page 16: ...MS Press the OK button from any histogram on screen to open the Histogram Explorer menu 01 Feb 18 14 16 Menu Home Histograms Settings Help 01 Feb 18 14 16 Histogram Test 3 03 Dec 17 Count 0 900 36 24...

Page 17: ...o printer Help Screens Tutorials 1 From the Menu screen scroll to Help press the OK button 2 Scroll to the topic of interest press the OK button 01 Feb 18 14 16 Scepter Software 4 6 01 Feb 18 14 16 Me...

Page 18: ...ration of cell sample is within recommended operating range See Cell Bead and Particle Suspensions Test sample using Scepter 3 0 Test Beads Cat No PHCC3BEADS to ensure unit operating properly for conc...

Page 19: ...y needs to be re charged Place Scepter 3 0 on charging stand to recharge the instrument See Battery p 5 Battery is not charging Ensure that the unit is correctly positioned in charging stand An animat...

Page 20: ...ressing of OK button Fully submerge sensor in sample before pressing OK button Air bubble in sample Mix sample gently to avoid air bubbles Sensor lifted from sample before screen displays Sample Loade...

Page 21: ...ed on FAT32 formatted USB thumb drive and try again See USB Thumb Drive Formatting p 8 Software update blocked on battery low Insufficient battery to perform update Recharge Scepter 3 0 and try again...

Page 22: ...ntal Conditions Temperature 15 C to 25 C Maximum Relative Humidity 20 80 non condensing Storage Conditions Temperature 15 C to 30 C Maximum Relative Humidity 20 80 non condensing Scepter 3 0 Sensor Ma...

Page 23: ...p E GP Sterile Vacuum Filtration System Millipore Express PLUS PES 0 22 500 38 12 SEGPU0538 0 22 500 45 12 SEGPU0545 0 22 1000 38 12 SEGPU1138 0 22 1000 45 12 SEGPU1145 Steritop E GP Sterile Funnel le...

Page 24: ...Merck KGaA Darmstadt Germany and or its affiliates All Rights Reserved Global Registration Marks of Conformity USA TFB 1003 EUROPE C TAIWAN JAPAN R209 J00212 SOUTH KOREA R C Emd PHCC30000 RUSSIA BELAR...

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