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00110230w  Rev 10/20 

19 of 24

Symptom

General Cause

Corrective Action

Sensor does not fit  
into sensor port

Incorrect sensor orientation or 
Sensor 2.0 stock.

Make sure the sensor size number and laminated circuit are 
facing the front of the instrument. See 

Sensors, p .11 .

Only Scepter™ 3.0 Sensors can be used.

Aperture block

Wrong diluent, clogging, air 
bubble, debris

Refer to list of acceptable diluents in the 

Cell, Bead, and 

Particle Suspensions, p .12 

and more detail in Error 

Messages: 

Aperture block, p .20

.

Test timeout

Sample may be clumpy

Re-insert sensor. Try new sensor or pre-filter using Steriflip

®

 

filter unit with 20 µm nylon net. If problem persists, contact 
Tech Service.

Sensor issue

No response on  
display when pressing 
control buttons

Internal software issue

Power off by pressing menu botton 3-5 seconds, until it shuts 
down. Restart after 10 seconds, retry previous action.

Unable to reach website 
using the Scepter™ 3.0  
IP address

Not on the same network or 
network firewall setting 
blocking

Ensure the computer is on the same network as the Scepter 3.0. 
If problem persists, check with your network administrator to 
ensure LAN to LAN connections are not blocked

Error Messages

Message

General Cause

Corrective Action

Battery  
needs recharging! 

Please recharge 
 the instrument! 

Battery is critically low! 

System is powering down.

Low battery/no battery

Battery needs to be re-charged. Place Scepter™ 3.0 on  
charging stand to recharge the instrument. See 

Battery, p .5

.

Battery is not charging

Ensure that the unit is correctly positioned in charging stand.  
An animated ascending power bar should be looping  
if connected properly. 
Ensure the charging station is plugged into a working  
AC wall outlet.
•  If instrument is on or asleep, display will flash when on 

charging stand and beep.

•  If instrument is fully off, it will recharge but will not display  

on screen until turned on.

If battery is completely drained, place on charging stand  
and press the MENU button for several seconds until the  
vibrant M is seen on screen. If no vibrant M appears,  
contact Tech Service. 

Memory Almost Full

Delete Histograms or 
Export to USB thumb drive

Nearly 999 histograms  
have been saved

Move saved histograms to USB thumb drive or upload to 
website. Delete historgrams once the transfer has been 
confirmed. Deleted histograms cannot be restored.

Memory Full

Instrument has exceeded 
maximum storage capacity  
of 999 saved histograms 

Unhandled exception

Internal software issue due 
to the instrument malfunction 
or high level of external 
interference

Internal software issue due to the instrument malfunction or 
high level of external interference.

Lost Sensor

Sensor was not  
properly inserted

Follow steps in 

Sensors .

Sensor removed or  
ejected prematurely

Histogram data not 
displayed on screen 

Sensor tip still installed in unit

Eject sensor to continue.

Please eject the Sensor  
to continue

Used Sensor is in 
Scepter™ 3.0

Eject sensor to continue.

Summary of Contents for PHCC30000

Page 1: ...perates as MilliporeSigma in the U S and Canada The life science business of Merck KGaA Darmstadt Germany operates as MilliporeSigma in the US and Canada SigmaAldrich com User Guide Scepter 3 0 Handhe...

Page 2: ...r computer easily No more cables The new Charging Station stores Scepter 3 0 out of the way when not in use freeing up valuable hood or lab bench space This system is intended for research use only Co...

Page 3: ...nstrument to vibrations Vibrations may cause instrument malfunction or damage Do not autoclave or expose to high temperature To avoid damage to internal electronic components do not spray instrument w...

Page 4: ...s the sensor Tech Service Port For Service use only USB Port Connects instrument to USB thumb drive Ergonomic Hook Allows comfortable handling of the instrument Secures the instrument to charger Contr...

Page 5: ...the Recharging screen when placed in the Charging Station the following steps must be followed to start charging the Scepter 3 0 battery 1 Place Scepter 3 0 into the Charging Station 2 While on the s...

Page 6: ...kable surface 1 Use double sided mounting tape 2 Remove tape liner from one side of tape and affix to back of mounting plate as shown below 3 Remove remaining tape liner and press firmly onto glass me...

Page 7: ...14 16 Edit Time Hours Minutes Seconds 14 16 34 Press OK to accept 01 Feb 18 14 16 Edit Time Hours Minutes Seconds 14 16 34 Press OK to accept 1 Scroll to Edit Time and press the OK button 2 Scroll to...

Page 8: ...nd select Properties If the File system is identified as FAT32 no formatting is needed Continue to Creating a Wi Fi Configuration File NOTE This view will also show how much space is available on your...

Page 9: ...file from the computer onto the USB thumb drive Once the file has loaded remove the USB thumb drive from the computer port Connect to Wi Fi Network 1 From the Main Menu select Settings and press the...

Page 10: ...were last set by the user either during a count or during post count data management 01 Feb 18 14 16 Menu Home Histograms Settings Help 01 Feb 18 14 16 Settings Back Date Time Bluetooth Wifi Gating M...

Page 11: ...epter 3 0 instrument Do not use sensor if visibly damaged Attach the Sensor 1 When the Scepter 3 0 is powered on it will show Attach the Sensor to begin 2 Holding the sensor with the sensor size numbe...

Page 12: ...net is recommend use low vacuum 5 Hg Cell Bead and Particle Suspensions Materials Required Cell sample Scepter 3 0 Test Beads Cat No PHCC3BEADS or particle suspensions Note Some particle suspensions...

Page 13: ...ion and diameter or volume will be displayed on the screen Press the OK button to save up to 999 histograms in the Scepter 3 0 memory and open the Histogram Explorer Press the OK button to return to H...

Page 14: ...03 Dec 17 Count 0 900 36 24 30 18 12 6 Dia in m 3 342e5 15 685 mL m Conc Dia 01 Feb 18 14 16 Histogram Edit Gates High gate Done 26 575 Low gate 12 585 Count 0 900 36 24 30 18 12 6 Dia in m Switch to...

Page 15: ...wirelessly Materials Required Wireless Network broadcasting at 2 4 Ghz PC Mac or UNIX Computer connected to the same Wi Fi Network as Scepter 3 0 Scepter 3 0 Instrument Follow Wi Fi Network p 8 direct...

Page 16: ...MS Press the OK button from any histogram on screen to open the Histogram Explorer menu 01 Feb 18 14 16 Menu Home Histograms Settings Help 01 Feb 18 14 16 Histogram Test 3 03 Dec 17 Count 0 900 36 24...

Page 17: ...o printer Help Screens Tutorials 1 From the Menu screen scroll to Help press the OK button 2 Scroll to the topic of interest press the OK button 01 Feb 18 14 16 Scepter Software 4 6 01 Feb 18 14 16 Me...

Page 18: ...ration of cell sample is within recommended operating range See Cell Bead and Particle Suspensions Test sample using Scepter 3 0 Test Beads Cat No PHCC3BEADS to ensure unit operating properly for conc...

Page 19: ...y needs to be re charged Place Scepter 3 0 on charging stand to recharge the instrument See Battery p 5 Battery is not charging Ensure that the unit is correctly positioned in charging stand An animat...

Page 20: ...ressing of OK button Fully submerge sensor in sample before pressing OK button Air bubble in sample Mix sample gently to avoid air bubbles Sensor lifted from sample before screen displays Sample Loade...

Page 21: ...ed on FAT32 formatted USB thumb drive and try again See USB Thumb Drive Formatting p 8 Software update blocked on battery low Insufficient battery to perform update Recharge Scepter 3 0 and try again...

Page 22: ...ntal Conditions Temperature 15 C to 25 C Maximum Relative Humidity 20 80 non condensing Storage Conditions Temperature 15 C to 30 C Maximum Relative Humidity 20 80 non condensing Scepter 3 0 Sensor Ma...

Page 23: ...p E GP Sterile Vacuum Filtration System Millipore Express PLUS PES 0 22 500 38 12 SEGPU0538 0 22 500 45 12 SEGPU0545 0 22 1000 38 12 SEGPU1138 0 22 1000 45 12 SEGPU1145 Steritop E GP Sterile Funnel le...

Page 24: ...Merck KGaA Darmstadt Germany and or its affiliates All Rights Reserved Global Registration Marks of Conformity USA TFB 1003 EUROPE C TAIWAN JAPAN R209 J00212 SOUTH KOREA R C Emd PHCC30000 RUSSIA BELAR...

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