4
5
20424816 Rev 06/21
20424816 Rev 06/21
English
English
Load a Recipe
Choose SETTING > RECIPE. Touch the Recipe
Information screen. Recipe select dialog box
will appear.
Select ”RECIPE NAME” > LOAD > OK > EXIT.
Note:
Confirm the desired Recipe name is
displayed on upper right of the screen.
Chip Assembly
PAGE Chip
E Chip
Cathode Side
Anode Side
1. Remove the white tape on the anode side
of the Chip.
2. Carefully remove the plastic cover on the
cathode side of the Chip.
3. Gently rinse anode and cathode buffer
wells with distilled water. Using a paper
cleaning wipe, carefully wipe any liquid
from the top of the Chip making sure not
to damage the thin strip of gel located
on the Cathode side of the Chip.
Insert the Chips Into the Tray
On the Auto2D
®
Device touch screen, choose
OPEN > OK. Once tray is open, place the
PAGE Chip
(light blue line)
with the anode side
in front. Place the Solution Chip Plus
(dark
blue lines)
on top of the PAGE Chip with the
cut-off corners in the front.
Anode Side
Cathode Side
IEF Chip
Remove the IEF Chip protective film.
Insert the IEF Chip in the slot indicated
by the white dot on the Electrode Chip Plus.
Before the Starting Eletrophoresis step, verify
the following:
• Electrode Chip Plus is not wet except for
anode and cathode chamber.
• The clear cover on the cathode side of the
PAGE Chip is removed.
• Solution has been added where required.
Start Electrophoresis
Choose CLOSE > OK > START > OK.
Electrophoresis will begin.
Apply the Solutions
6
a
7
3
4
3
b
c
6
2
a
5
b
1
3
1. Cathode Buffer, 4500 µL
2. Distilled Water, 4500 µL
3. Anode Buffer, 4000 µL
4. Working Equilibration Buffer:
a. 700 µL
b. 700 µL (optional)
5. Working Rehydration Solution, 100 µL
6. Sample, 13–15 µL
7. Filter Paper and Water*
5 µL, each
* Wetted filter paper can be used as a wick to
trap salt. Using filter paper is recommended
to enhance separation of protein samples
containing a high salt concentration when
using Auto2D
®
Plus programs and is required
when using Auto2D
®
Desalting program.
Filter paper should be 0.2–0.3 mm in
thickness and cut to a size of 8 mm x 4 mm.
Place the Electrode Chip Plus
Position the
towards the front.
