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Malvern MICROCAL PEAQ-ITC Operating Instructions Manual Download Page 46

Operati on

44

Note

: In most cases the MicroCal standard method can be used.

Instrument control bar

The experienced user can control the instrument from the instrument control bar located
at the bottom of the MicroCal PEAQ-ITC Control Software window.

The instrument control bar can be used to execute all loading and cleaning tasks per-
formed by the instrument. This allows the user to perform loading and cleaning tasks
without referring to the software guided workflows.

For more information, see

MicroCal PEAQ-ITC User Manual

.

Experiment and method files

MicroCal PEAQ-ITC Control Software can open calorimetry data files (experiment files) or

method files. Method files only contain instrument settings. Experiment files also contain
calorimetric data from an experiment run. Parameters from both file types can be used to
create new experiment runs.

File type

File extension

Description

Experiment file

itc

Calorimetric data from MicroCal PEAQ-ITC.

Method file

itcm

Method file created with MicroCal PEAQ-
ITC Control Software or MicroCal PEAQ-ITC
Analysis Software.

Contains instrument settings only.

Summary of Contents for MICROCAL PEAQ-ITC

Page 1: ...alvern Worcestershire WR14 1XZ UK Tel 44 1684 892456 Fax 44 1684 892789 MICROCAL PEAQ ITC OPERATING INSTRUCTIONS LABEL FREE BINDING ANALYSIS MICROCALORIMETRY LABEL FREE BINDING ANALYSIS MICROCALORIMET...

Page 2: ......

Page 3: ...MICROCAL PEAQ ITC OPERATING INSTRUCTIONS MAN0572 01 EN 00...

Page 4: ...cation No reproduction or transmission of any part of this publication is allowed without the express written permission of Malvern Instruments Ltd Head office Malvern Instruments Ltd Enigma Business...

Page 5: ...s 19 Recycling information 20 Declaration of Hazardous Substances DoHS 20 System description 23 General introduction 24 System overview 25 Instrument cell unit 26 Washing module 30 Installation 33 Ins...

Page 6: ...syringe plunger tip 55 Replace the titration syringe 62 Clean the titration syringe 66 Refill the reference cell 71 Troubleshooting 73 Troubleshooting overview 74 Troubleshooting chart 75 Reference 77...

Page 7: ...ces regulatory information and a general description of the intended use of MicroCal PEAQ ITC and a list of associated documentation 5 1 The following topics are covered in this section About this man...

Page 8: ...vern InstrumentsFile menu Hardware items are identified in the text by bold text for example Malvern Instru mentsPower Important user information Note All users must read the entire Operating Instruct...

Page 9: ...er run ning Microsoft Windows in the version provided with your product l You should be acquainted with the use of general laboratory equipment and with handling of chemical and biological materials l...

Page 10: ...p contains useful information that can improve or optimize your pro cedures Regulatory information Introduction This section lists the directives and standards that the MicroCal PEAQ ITC system fulfil...

Page 11: ...erations described in the user doc umentation International standards The standard requirements fulfilled by this product are summarized in the table below Standard Description Notes EN 61010 1 IEC 61...

Page 12: ...andards requirements EN IEC Regulatory compliance of connected equipment Any equipment connected to MicroCal PEAQ ITC should meet the safety requirements of EN 61010 1 IEC 61010 1 or relevant harmoniz...

Page 13: ...els on the system and information regarding recycling are also described 11 2 WARNING Before installing operating or maintaining the product all users must read and understand the entire contents of t...

Page 14: ...precautions l Using flammable liquids l Personal protection l Installing and moving l System operation l Maintenance General precautions Warning Do not operate the product in any other way than descr...

Page 15: ...ctromagnetic and radio frequency interference EMI RFI Lab oratory tests have shown that significant electromagnetic waves may affect the performance of MicroCal PEAQ ITC Strong electrical or magnetic...

Page 16: ...uments Warning Electrical power Provide proper electrical power to the instrument This should be 100 240 VAC 50 60 Hz observing any local electrical safety requirements Only power cords supplied with...

Page 17: ...ous chem icals flush the entire system tubing with distilled water before service and maintenance Warning Methanol is highly volatile and can be hazardous to humans l Storage containers should be kept...

Page 18: ...ns from a technician Removal or modification of any cover or component could result in an unsafe or easily damaged instrument Warning Only spare parts and accessories that are approved or supplied by...

Page 19: ...ety Data Sheet Labels Introduction This section describes the various labels on the MicroCal PEAQ ITC instrument and their meaning Instrument label The following illustration shows an example of the i...

Page 20: ...disposed as unsorted municipal waste and must be collected separately Please con tact an authorized representative of the manufacturer for information concerning the decommissioning of equipment The...

Page 21: ...Power failure The following list describes what happens in the event of power failure l The MicroCal PEAQ ITC run is interrupted immediately l The data collected up to the time of the power failure i...

Page 22: ...regulations Recycling of hazardous substances MicroCal PEAQ ITC contains hazardous substances Detailed information is available from your Malvern Instruments representative Disposal of electrical com...

Page 23: ...not result in any severe environmental pollution any bodily injury or damage to any assets The unit of the period is Year In order to maintain the declared EFUP the product shall be operated normally...

Page 24: ...ed for this part is above the limit requirement in SJ T11363 2006 l Data listed in the table represents best information available at the time of pub lication SJ T11363 2006 l List of hazardous substa...

Page 25: ...N This section provides a description of MicroCal PEAQ ITC and an overview of its com ponents 23 3 The following topics are covered General introduction 24 System overview 25 Instrument cell unit 26 W...

Page 26: ...olved as a result of mixing precise amounts of reactants The sample and reference cells are made from Hastelloy a highly inert material Data analysis is performed using MicroCal PEAQ ITC Analysis Soft...

Page 27: ...erview The following illustration shows the MicroCal PEAQ ITC instrument with the washing module and the Controller PC 1 2 Part Description 1 MicroCal PEAQ ITC washing module 2 MicroCal PEAQ ITC cell...

Page 28: ...ng illustration shows the MicroCal PEAQ ITC cell unit Part Description 1 Sample cell 2 Injector tower 3 Pipette 4 Loading syringe 5 Fill Port Adaptor FPA 6 FPA Storage Location 7 Wash load station 8 T...

Page 29: ...tower top view The following illustration shows a top view of the injection tower of the MicroCal PEAQ ITC cell unit Part Description 1 Sample cell 2 Reference cell 3 Cell Location 4 Rest Location 5...

Page 30: ...on 28 Pipette The following illustration shows the MicroCal PEAQ ITC pipette unit Part Function 1 Rotating assembly 2 Plunger tip 3 Pipette housing 4 Syringe glass 5 Retaining nut 6 Syringe paddle ste...

Page 31: ...at the rear of the cell unit The following illustration shows the connections at the rear of the MicroCal PEAQ ITC cell unit Part Function 1 Power switch 2 24 VDC input from power supply AC plug 3 USB...

Page 32: ...ption 30 Washing module The following illustration shows the MicroCal PEAQ ITC washing module Part Function 1 Cell Cleaning Tool 2 Detergent bottle 3 Methanol bottle 4 Water bottle 5 Waste bottle 6 Ov...

Page 33: ...ashing module The following illustration shows the connections at the rear of the MicroCal PEAQ ITC washing module Part Function 1 Power switch 2 24 VDC input from power supply AC plug 3 USB connectio...

Page 34: ......

Page 35: ...on of MicroCal PEAQ ITC The following topics are covered in this section 33 4 Installation precautions 34 Site requirements 35 Unpacking and transport 37 Set up 37 Note Any equipment connected to the...

Page 36: ...instrument should be used Warning Protective ground The product must always be connected to a grounded power outlet Warning Do not block the ventilation inlets or outlets on the system Warning Access...

Page 37: ...wer switch must always be easy to access The power cord with plug must always be easy to dis connect Ambient environment Environmental fluctuations may cause subtle performance problems The following...

Page 38: ...in the MicroCal PEAQ ITC instrument is adequate for most laboratory environments some disturbances may affect the performance of the instru ment and it may be necessary to have the AC power source ev...

Page 39: ...ocal Malvern Instruments representative Moving the system Notice Lift the product Lift the MicroCal PEAQ ITC instrument in the upright position 1 Disconnect the power cord 2 Disconnect all cables and...

Page 40: ......

Page 41: ...required to safely operate the MicroCal PEAQ ITC 39 5 The following topics are covered in this section Precautions 40 Prepare the instrument 41 Open a method or an experiment 43 Load the instrument 45...

Page 42: ...ure may cause the syringe to break and the exposure to hazardous sub stances may increase WARNING Placement of vessels containing liquid Do not place vessels con taining liquid on top of the instrumen...

Page 43: ...l oper ating orientation Other orientations will subject delicate sensors inside the instrument to stress Prepare the instrument Introduction This section describes the procedures needed to prepare th...

Page 44: ...oftware follow the steps described in the fol lowing table Step Action 1 Start the computer and log in to Windows 2 Turn on theMicroCal PEAQ ITC instrument using the Power switch at the rear of the un...

Page 45: ...ow to open a predefined method or experiment in the MicroCal PEAQ ITC Control Software Open a method or experiment In the Start Experiment workspace open a method file or an experiment file l Click Me...

Page 46: ...tware guided workflows For more information see MicroCal PEAQ ITC User Manual Experiment and method files MicroCal PEAQ ITC Control Software can open calorimetry data files experiment files or method...

Page 47: ...n syringe Addi tionally the user has the option of watching videos detailing the procedure Step Action 1 Click Load Result The Load workspace opens 2 To view a instruction video showing both sample ce...

Page 48: ...re displayed in the left Experiment Information pane Real time experiment status is displayed in the right pane Experiment information l Enter titrant concentration in the Syr M box l Enter sample cel...

Page 49: ...k Start in the right live pane to start the experiment The user will be promp ted to enter a filename and save location for the itc file Follow the experiment run in real time The instrument status is...

Page 50: ...ge Addi tionally the user has the option of watching videos detailing the procedure Step Action 1 Click Clean Result The Clean workspace opens 2 To view an instruction video showing all the default cl...

Page 51: ...Operation 49 Step Action Note Software instrument connection is required to clean the instrument...

Page 52: ......

Page 53: ...For a more detailed description of maintenance procedures watch the videos provided with the software in English only 51 6 The following topics are covered Maintenance overview 52 Replace the syringe...

Page 54: ...tective measures such as wearing protective glasses and gloves resistant to the substances used Follow local and or national regulations for safe operation and maintenance of MicroCal PEAQ ITC WARNING...

Page 55: ...wear protective glasses gloves and other suitable personal protective equipment as specified in the Safety Data Sheet Maintenance tools The following tools are used for MicroCal PEAQ ITC maintenance...

Page 56: ...workspace click Maintenance and the workspace name The following instruction is an example only and displays how to open the Change Plun ger Tip workspace Step Action 1 Click Maintenance 2 Click Chan...

Page 57: ...PEAQ ITC Control Software Note Instrument connection is required to replace the syringe plunger tip The Change Plunger Tip workflow The software guided The Change Plunger Tip workflow is divided into...

Page 58: ...he following table Step Action 1 Make sure that the Load Location does not contain a microcentrifuge tube 2 Move the pipette to the Load Location Note It should not be necessary but have your hand rea...

Page 59: ...1 Carefully remove the syringe glass using the supplied tweezers If the plunger motion did not expose enough syringe glass use the Syringe Glass Removal Tool 2 Insert the Plunger Tip Removal Tool into...

Page 60: ...er tip To install the plunger tip follow the instruction video and or the software guided work flow integrated in the MicroCal PEAQ ITC Control Software The software guided workflow is also shown in t...

Page 61: ...nction 1 Plunger tip 2 Plunger Tip Install Tool The plunger tip opening should face out from the tool 2 Insert the tool into the pipette housing You will meet resistance as the new plastic tip slides...

Page 62: ...follow the instruction video and or the software guided work flow integrated in the MicroCal PEAQ ITC Control Software The software guided workflow is also shown in the following table Step Action 1...

Page 63: ...the retaining nut in the Clean Location and insert the pipette into the Clean Loca tion without fully engaging the clamp 4 Start to reinstall the retaining nut by turning the pipette s rotating assemb...

Page 64: ...e the titration syringe follow the instruction video and or the software guided workflow integrated in the MicroCal PEAQ ITC Control Software The software guided workflow is also shown in the followin...

Page 65: ...e MicroCal PEAQ ITC Control Software The software guided workflow is also shown in the following table Step Action 1 Make sure that the Load Location does not contain a microcentrifuge tube 2 Move the...

Page 66: ...ss removal tool if the plunger motion did not expose enough syringe glass Install the syringe glass To install the syringe glass follow the instruction video and or the software guided work flow integ...

Page 67: ...ly the installed syringe glass should leave approximately 2 mm of glass exposed 3 Leave the retaining nut in the Clean Location and insert the pipette into the Clean Loca tion without fully engaging t...

Page 68: ...ion and maintenance of MicroCal PEAQ ITC To clean the titration syringe follow the instruction video and or the software guided workflow integrated in the MicroCal PEAQ ITC Control Software The softwa...

Page 69: ...uction video and or the soft ware guided workflow integrated in the MicroCal PEAQ ITC Control Software The software guided workflow is also shown in the following table Step Action 1 Make sure that th...

Page 70: ...se the syringe glass removal tool if the plunger motion did not expose enough syringe glass Clean the syringe glass Clean the glass syringe occasionally by hand as the instrument does not clean the en...

Page 71: ...crub the inside of the syringe with detergent using the supplied Syringe Glass Cleaning Brush 3 Rinse thoroughly with water to remove as much detergent as possible 4 Dry the syringe to prevent sample...

Page 72: ...the retaining nut in the Clean Location and insert the pipette into the Clean Loca tion without fully engaging the clamp 4 Start to reinstall the retaining nut by turning the pipette s rotating assemb...

Page 73: ...amilton syringe into the reference cell until it touches the bottom 2 Empty the reference cell completely by pulling up the syringe plunger Note Make sure that no bubbles are trapped in the cell 3 Rem...

Page 74: ...ith several abrupt spurts of the water solution Note Make sure no bubbles are transported into the reference cell while loading the water solution 7 Lift the tip of the syringe to the cell port just b...

Page 75: ...TROUBLESHOOTING This section provides information on how to solve problems that may arise 73 7 The following topics are covered Troubleshooting overview 74 Troubleshooting chart 75...

Page 76: ...is best to attach the raw data file itc Diagnosing the problem Perform the following minimum diagnostic steps prior to requesting service Step Action 1 Run a thorough cleaning routine with detergent...

Page 77: ...leaking and that the sample containers are properly inserted l Watch a cleaning and loading cycle for any obvious problems l Check that the Fill Port Adapter that con nects to the titration syringe i...

Page 78: ......

Page 79: ...tion that may be useful when installing operating maintaining and troubleshooting the MicroCal PEAQ ITC system 77 8 The following topics are covered in this section MicroCal PEAQ ITC specifications 78...

Page 80: ...ecifications Property Function Electrical ratings Voltage Frequency Power 100 240 VAC power adapter 24 VDC power supply to the instrument 50 60 Hz 150 W Output Secondary Data connection only Mode of o...

Page 81: ...tible chemicals Note A user can be exposed to large volumes of chemical substances over a long time period Material Safety Data Sheets MSDS provide the user with information regarding characteristics...

Page 82: ...Introduction This section specifies the wetted materials of MicroCal PEAQ ITC Wetted surface materials The following wetted surface materials are used in MicroCal PEAQ ITC l Hastelloy C276 l 316 SST...

Page 83: ...rical power 36 Emergency procedures 19 situation 19 Environment operating requirements 35 Experiment start 47 Experiment files 44 open 43 F Flammable liquids precautions 13 G General precautions 12 H...

Page 84: ...unit 28 Power failure 19 restart 19 Precautions flammable liquids 13 general 12 installing and moving 14 maintenance 16 52 personal protection 13 system operation 15 40 Prerequisites 7 Purpose of this...

Page 85: ...83 Troubleshooting chart 75 Typographical conventions 6 U Unpacking 37 User documentation 10 User information important 6 W Washing module 30 connections at the rear 31 Wetted materials 80...

Page 86: ...alvern Worcestershire WR14 1XZ UK Tel 44 1684 892456 Fax 44 1684 892789 MICROCAL PEAQ ITC OPERATING INSTRUCTIONS LABEL FREE BINDING ANALYSIS MICROCALORIMETRY LABEL FREE BINDING ANALYSIS MICROCALORIMET...

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